Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing
Analyte quantification in highly scattering media such as tissue, blood, and other biological fluids is challenging using conventional spectroscopic methods. Ultrasound easily penetrates these opaque samples, yet currently provides little chemical information. We have developed a general approach fo...
Gespeichert in:
| Veröffentlicht in: | Talanta (Oxford) 2011-02, Vol.83 (5), p.1364-1370 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1370 |
|---|---|
| container_issue | 5 |
| container_start_page | 1364 |
| container_title | Talanta (Oxford) |
| container_volume | 83 |
| creator | Dion, Jonathan R. Burns, David H. |
| description | Analyte quantification in highly scattering media such as tissue, blood, and other biological fluids is challenging using conventional spectroscopic methods. Ultrasound easily penetrates these opaque samples, yet currently provides little chemical information. We have developed a general approach for creating hydrogel biosensors based on antibody-linked cellulose polymers. Target recognition induces changes to the sensor stiffness and size, which is accompanied by characteristic changes to a measured ultrasonic frequency profile. Using this technique, nM sensitivity for acetaminophen is demonstrated in a series of biofluids including whole blood, blood plasma, saliva, and urine. Likewise, this methodology is attractive for point of care diagnostics due to the short measurement time, simple methodology which excludes pretreatment of samples, and has minimal chemical or buffer requirements. |
| doi_str_mv | 10.1016/j.talanta.2010.11.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_856782126</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0039914010008702</els_id><sourcerecordid>856782126</sourcerecordid><originalsourceid>FETCH-LOGICAL-c426t-dfebaac122f9f2221855a74d577d9d42b25fca5bf288160f87dde882426b7ee73</originalsourceid><addsrcrecordid>eNqFkc2OFCEUhYnROO3oI2jYGFfVwqUoqJUxE_-SSdw4a0LBZaStLlqgJ6m3l063upzVIeQ7cO85hLzmbMsZH97vttXOdql2C-x0x7dNnpAN10p0QirxlGwYE2M38p5dkRel7BhjIJh4Tq6Ag9AKYEP83VyzLWmJjoaMv4-4uJXaxc5riYWm0M41Tsmv3RyXX-jpz9XndI8znWIquJSUCw0p02wP0dNDiks92ZzNSCuWGpf7l-RZsHPBVxe9JnefP_24-drdfv_y7ebjbed6GGrnA07WOg4QxgAAXEtpVe-lUn70PUwgg7NyCqA1H1jQynvUGpp5UohKXJN353cPObVNSjX7WBzOLSdMx2K0HJRuuw-Pk73ouZDAGinPpMuplIzBHHLc27wazsypCbMzlybMqQnDuWnSfG8uPxynPfp_rr_RN-DtBbDF2Tlku7hY_nNCw6iUbNyHM4ctuYeI2RQXW0voY0ZXjU_xkVH-AIErqwQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>843413520</pqid></control><display><type>article</type><title>Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Dion, Jonathan R. ; Burns, David H.</creator><creatorcontrib>Dion, Jonathan R. ; Burns, David H.</creatorcontrib><description>Analyte quantification in highly scattering media such as tissue, blood, and other biological fluids is challenging using conventional spectroscopic methods. Ultrasound easily penetrates these opaque samples, yet currently provides little chemical information. We have developed a general approach for creating hydrogel biosensors based on antibody-linked cellulose polymers. Target recognition induces changes to the sensor stiffness and size, which is accompanied by characteristic changes to a measured ultrasonic frequency profile. Using this technique, nM sensitivity for acetaminophen is demonstrated in a series of biofluids including whole blood, blood plasma, saliva, and urine. Likewise, this methodology is attractive for point of care diagnostics due to the short measurement time, simple methodology which excludes pretreatment of samples, and has minimal chemical or buffer requirements.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2010.11.010</identifier><identifier>PMID: 21238722</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analyte recognition ; Analytical chemistry ; Animals ; Antibodies - chemistry ; Antibody ; Biological and medical sciences ; Biosensing Techniques ; Biosensor ; Biosensors ; Biotechnology ; Blood Chemical Analysis ; Cattle ; Chemistry ; Clinical Chemistry Tests ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Humans ; Hydrogel ; Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry ; Methods. Procedures. Technologies ; Point-of-care ; Point-of-Care Systems ; Rats ; Sensitivity and Specificity ; Ultrasonics ; Ultrasound ; Urinalysis ; Various methods and equipments</subject><ispartof>Talanta (Oxford), 2011-02, Vol.83 (5), p.1364-1370</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-dfebaac122f9f2221855a74d577d9d42b25fca5bf288160f87dde882426b7ee73</citedby><cites>FETCH-LOGICAL-c426t-dfebaac122f9f2221855a74d577d9d42b25fca5bf288160f87dde882426b7ee73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2010.11.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23829775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21238722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dion, Jonathan R.</creatorcontrib><creatorcontrib>Burns, David H.</creatorcontrib><title>Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>Analyte quantification in highly scattering media such as tissue, blood, and other biological fluids is challenging using conventional spectroscopic methods. Ultrasound easily penetrates these opaque samples, yet currently provides little chemical information. We have developed a general approach for creating hydrogel biosensors based on antibody-linked cellulose polymers. Target recognition induces changes to the sensor stiffness and size, which is accompanied by characteristic changes to a measured ultrasonic frequency profile. Using this technique, nM sensitivity for acetaminophen is demonstrated in a series of biofluids including whole blood, blood plasma, saliva, and urine. Likewise, this methodology is attractive for point of care diagnostics due to the short measurement time, simple methodology which excludes pretreatment of samples, and has minimal chemical or buffer requirements.</description><subject>Analyte recognition</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Antibodies - chemistry</subject><subject>Antibody</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensor</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Blood Chemical Analysis</subject><subject>Cattle</subject><subject>Chemistry</subject><subject>Clinical Chemistry Tests</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Humans</subject><subject>Hydrogel</subject><subject>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</subject><subject>Methods. Procedures. Technologies</subject><subject>Point-of-care</subject><subject>Point-of-Care Systems</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><subject>Ultrasonics</subject><subject>Ultrasound</subject><subject>Urinalysis</subject><subject>Various methods and equipments</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2OFCEUhYnROO3oI2jYGFfVwqUoqJUxE_-SSdw4a0LBZaStLlqgJ6m3l063upzVIeQ7cO85hLzmbMsZH97vttXOdql2C-x0x7dNnpAN10p0QirxlGwYE2M38p5dkRel7BhjIJh4Tq6Ag9AKYEP83VyzLWmJjoaMv4-4uJXaxc5riYWm0M41Tsmv3RyXX-jpz9XndI8znWIquJSUCw0p02wP0dNDiks92ZzNSCuWGpf7l-RZsHPBVxe9JnefP_24-drdfv_y7ebjbed6GGrnA07WOg4QxgAAXEtpVe-lUn70PUwgg7NyCqA1H1jQynvUGpp5UohKXJN353cPObVNSjX7WBzOLSdMx2K0HJRuuw-Pk73ouZDAGinPpMuplIzBHHLc27wazsypCbMzlybMqQnDuWnSfG8uPxynPfp_rr_RN-DtBbDF2Tlku7hY_nNCw6iUbNyHM4ctuYeI2RQXW0voY0ZXjU_xkVH-AIErqwQ</recordid><startdate>20110215</startdate><enddate>20110215</enddate><creator>Dion, Jonathan R.</creator><creator>Burns, David H.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QH</scope><scope>7QO</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20110215</creationdate><title>Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing</title><author>Dion, Jonathan R. ; Burns, David H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-dfebaac122f9f2221855a74d577d9d42b25fca5bf288160f87dde882426b7ee73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analyte recognition</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Antibodies - chemistry</topic><topic>Antibody</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques</topic><topic>Biosensor</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Blood Chemical Analysis</topic><topic>Cattle</topic><topic>Chemistry</topic><topic>Clinical Chemistry Tests</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Humans</topic><topic>Hydrogel</topic><topic>Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry</topic><topic>Methods. Procedures. Technologies</topic><topic>Point-of-care</topic><topic>Point-of-Care Systems</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><topic>Ultrasonics</topic><topic>Ultrasound</topic><topic>Urinalysis</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dion, Jonathan R.</creatorcontrib><creatorcontrib>Burns, David H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Aqualine</collection><collection>Biotechnology Research Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dion, Jonathan R.</au><au>Burns, David H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2011-02-15</date><risdate>2011</risdate><volume>83</volume><issue>5</issue><spage>1364</spage><epage>1370</epage><pages>1364-1370</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>Analyte quantification in highly scattering media such as tissue, blood, and other biological fluids is challenging using conventional spectroscopic methods. Ultrasound easily penetrates these opaque samples, yet currently provides little chemical information. We have developed a general approach for creating hydrogel biosensors based on antibody-linked cellulose polymers. Target recognition induces changes to the sensor stiffness and size, which is accompanied by characteristic changes to a measured ultrasonic frequency profile. Using this technique, nM sensitivity for acetaminophen is demonstrated in a series of biofluids including whole blood, blood plasma, saliva, and urine. Likewise, this methodology is attractive for point of care diagnostics due to the short measurement time, simple methodology which excludes pretreatment of samples, and has minimal chemical or buffer requirements.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21238722</pmid><doi>10.1016/j.talanta.2010.11.010</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-9140 |
| ispartof | Talanta (Oxford), 2011-02, Vol.83 (5), p.1364-1370 |
| issn | 0039-9140 1873-3573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_856782126 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analyte recognition Analytical chemistry Animals Antibodies - chemistry Antibody Biological and medical sciences Biosensing Techniques Biosensor Biosensors Biotechnology Blood Chemical Analysis Cattle Chemistry Clinical Chemistry Tests Exact sciences and technology Fundamental and applied biological sciences. Psychology General, instrumentation Humans Hydrogel Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry Methods. Procedures. Technologies Point-of-care Point-of-Care Systems Rats Sensitivity and Specificity Ultrasonics Ultrasound Urinalysis Various methods and equipments |
| title | Ultrasonic frequency analysis of antibody-linked hydrogel biosensors for rapid point of care testing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T09%3A41%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultrasonic%20frequency%20analysis%20of%20antibody-linked%20hydrogel%20biosensors%20for%20rapid%20point%20of%20care%20testing&rft.jtitle=Talanta%20(Oxford)&rft.au=Dion,%20Jonathan%20R.&rft.date=2011-02-15&rft.volume=83&rft.issue=5&rft.spage=1364&rft.epage=1370&rft.pages=1364-1370&rft.issn=0039-9140&rft.eissn=1873-3573&rft.coden=TLNTA2&rft_id=info:doi/10.1016/j.talanta.2010.11.010&rft_dat=%3Cproquest_cross%3E856782126%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=843413520&rft_id=info:pmid/21238722&rft_els_id=S0039914010008702&rfr_iscdi=true |