Synthesis of double-layered rotavirus-like particles using internal ribosome entry site vector system in stably-transformed Drosophila melanogaster

We established a bicistronic expression system using an encephalomyocarditis virus (EMCV)-derived internal ribosomal entry site (IRES) element to generate stably transformed Drosophila melanogaster Schneider 2 (S2) cells expressing human rotavirus Wa capsid proteins, VP2 and VP6, for the synthesis o...

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Veröffentlicht in:	Biotechnology letters 2011, Vol.33 (1), p.41-46
Hauptverfasser:	Lee, Jong Min, Chung, Ha Young, Kim, Kyung Il, Yoo, Ki Hyun, Hwang-Bo, Jeon, Chung, In Sik, Park, Jong-Hwa
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Viral Antigens, Viral - genetics Antigens, Viral - metabolism Applied Microbiology Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Biotechnology - methods Capsid Proteins Capsid Proteins - genetics Capsid Proteins - metabolism Cardiovirus A Cell Line Chemical synthesis coat proteins Double-layered virus-like particle Drosophila Drosophila melanogaster Encephalomyocarditis virus Encephalomyocarditis virus - genetics Fundamental and applied biological sciences. Psychology Genetic Engineering Genetic Engineering - methods genetics Human rotavirus humans Insects Internal ribosome entry site Life Sciences Mathematical analysis metabolism methods Microbiology Original Research Paper Protein Biosynthesis Proteins Recombinant Recombinant Proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Ribonucleic acid ribosomes RNA Rotavirus Synthesis Translations Vectors (mathematics) Virology Virology - methods Virosomes Virosomes - genetics Virosomes - metabolism
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