Mitochondrial DNA Alterations in Colorectal Cancer Cell Lines
Somatic mutations of mitochondrial DNA (mtDNA) have been reported in different types of cancers and are suggested to play roles in metastasis, cancer development and response to anticancer agents. To predict potential roles of mtDNA alterations in colorectal cancer, we determined the entire mtDNA se...
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| Veröffentlicht in: | Journal of Nippon Medical School 2011, Vol.78(1), pp.13-21 |
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| container_title | Journal of Nippon Medical School |
| container_volume | 78 |
| creator | Chihara, Naoto Amo, Taku Tokunaga, Akira Yuzuriha, Ryo Wolf, Alexander M. Asoh, Sadamitsu Suzuki, Hideyuki Uchida, Eiji Ohta, Shigeo |
| description | Somatic mutations of mitochondrial DNA (mtDNA) have been reported in different types of cancers and are suggested to play roles in metastasis, cancer development and response to anticancer agents. To predict potential roles of mtDNA alterations in colorectal cancer, we determined the entire mtDNA sequence of eleven human-derived colorectal cancer cell lines and compared with the revised Cambridge Reference Sequence to identify nucleotide alterations. Four homoplasmic and six heteroplasmic alterations were found to be novel. Among them, homoplasmic G6709A (MT-CO1) and G14804A (MT-CYB) alterations cause amino acid changes in the highly conserved residues. Heteroplasmic G1576A (MT-RNR1) and G2975A (MT-RNR2) alterations are expected to make the stem structure of mitochondrial ribosomal RNAs unstable. These nucleotide alterations are candidates that could play important roles in cancer. |
| doi_str_mv | 10.1272/jnms.78.13 |
| format | Article |
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To predict potential roles of mtDNA alterations in colorectal cancer, we determined the entire mtDNA sequence of eleven human-derived colorectal cancer cell lines and compared with the revised Cambridge Reference Sequence to identify nucleotide alterations. Four homoplasmic and six heteroplasmic alterations were found to be novel. Among them, homoplasmic G6709A (MT-CO1) and G14804A (MT-CYB) alterations cause amino acid changes in the highly conserved residues. Heteroplasmic G1576A (MT-RNR1) and G2975A (MT-RNR2) alterations are expected to make the stem structure of mitochondrial ribosomal RNAs unstable. 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These nucleotide alterations are candidates that could play important roles in cancer.</description><subject>Amino Acid Sequence</subject><subject>Amino Acid Substitution</subject><subject>Cell Line, Tumor</subject><subject>colorectal cancer</subject><subject>Colorectal Neoplasms - genetics</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Cytochromes b - genetics</subject><subject>DNA, Mitochondrial - chemistry</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Electron Transport Complex IV - genetics</subject><subject>Humans</subject><subject>mitochondria</subject><subject>Mitochondrial Proteins - genetics</subject><subject>Molecular Sequence Data</subject><subject>mtDNA</subject><subject>Mutation</subject><subject>nucleotide sequence</subject><subject>Point Mutation</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><issn>1345-4676</issn><issn>1347-3409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1Lw0AQhhdRbK1e_AGSmyCk7mY_cxAp8ROqXvS8bDcTm5Ls1t304L83NbVeZgbeh5fhQeic4CnJZHa9cm2cSjUl9ACNCWUypQznh783T5mQYoROYlxhTCnn4hiNMkJVLhgdo5uXuvN26V0ZatMkd6-zZNZ0EExXexeT2iWFb3wA2_VpYZyFkBTQNMm8dhBP0VFlmghnuz1BHw_378VTOn97fC5m89RykXepwotKEiNsyTMMjNOSYADgJq9ynDGqjMALLFiuKiVshXPCKLWyNBmXTADQCbocetfBf20gdrqto-3fMA78JmrFhZSMZaInrwbSBh9jgEqvQ92a8K0J1ltbemtLS6UJ7eGLXe1m0UK5R__09MDtAKxiZz5hD5jQ1baB_65hELpP7NIEDY7-ALB0e4Q</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Chihara, Naoto</creator><creator>Amo, Taku</creator><creator>Tokunaga, Akira</creator><creator>Yuzuriha, Ryo</creator><creator>Wolf, Alexander M.</creator><creator>Asoh, Sadamitsu</creator><creator>Suzuki, Hideyuki</creator><creator>Uchida, Eiji</creator><creator>Ohta, Shigeo</creator><general>The Medical Association of Nippon Medical School</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Mitochondrial DNA Alterations in Colorectal Cancer Cell Lines</title><author>Chihara, Naoto ; 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| subjects | Amino Acid Sequence Amino Acid Substitution Cell Line, Tumor colorectal cancer Colorectal Neoplasms - genetics Colorectal Neoplasms - pathology Cytochromes b - genetics DNA, Mitochondrial - chemistry DNA, Mitochondrial - genetics Electron Transport Complex IV - genetics Humans mitochondria Mitochondrial Proteins - genetics Molecular Sequence Data mtDNA Mutation nucleotide sequence Point Mutation Sequence Analysis, DNA Sequence Homology, Amino Acid |
| title | Mitochondrial DNA Alterations in Colorectal Cancer Cell Lines |
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