High-throughput DNA extraction for PCR-based genotyping of single Penaeus monodon embryos and nauplii

A simple technique is described for isolating DNA from individual embryos or nauplii of the Black Tiger shrimp, Penaeus monodon. The protocol used a commercial prepGEM™ insect DNA extraction kit in combination with water bath ultrasonic disruption. DNA quality and yields were sufficient for multiple...

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Veröffentlicht in:	Aquaculture 2010-12, Vol.310 (1), p.61-65
Hauptverfasser:	Rao, Min, Arnold, Stuart J., Cowley, Jeff A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal aquaculture Animal productions Biological and medical sciences Black Tiger shrimp Fertilization Fundamental and applied biological sciences. Psychology General aspects Giant Tiger shrimp Marine Penaeus monodon Reproduction
Online-Zugang:	Volltext
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