Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma
A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a...
Gespeichert in:
| Veröffentlicht in: | Journal of AOAC International 2011, Vol.94 (1), p.136-142 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 142 |
|---|---|
| container_issue | 1 |
| container_start_page | 136 |
| container_title | Journal of AOAC International |
| container_volume | 94 |
| creator | LARGER MANFIO, Josélia SANTOS, Verônica Jorge LANCHOTE, Vera Lúcia SANTOS, Luciana M CARMONA, Maria José C AULER, José Otavio BRUM JUNIOR, Liberato DONADUZZI, Carmen Maria SANTOS, Silvia R. C. J |
| description | A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery. |
| doi_str_mv | 10.1093/jaoac/94.1.136 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_856770627</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A258599343</galeid><sourcerecordid>A258599343</sourcerecordid><originalsourceid>FETCH-LOGICAL-c522t-93dd854dd37d324bdd25d3ae3d0582a5b663ada5647150711c02c6e1569e74113</originalsourceid><addsrcrecordid>eNptkd9rFDEQxxex2Nr66qMsSPFp7_J7N4_lqla4o4Wqr2EumfVSdpMz2RX8703bs30pGUgYPvMhzLeq3lOyoETz5R1EsEstFnRBuXpVnVAtRNNqxl6XN1Gk4aylx9XbnO8IEVQR9qY6ZpRrKjQ5qfIl_sEh7kcMUw3B1T9h8A4mH0Md-9Kpr27Wq-XmtlS9wWkXXd3HVE87rC9xwjT68ETfzn3RQPDDg2oT037nA9a-WOaxuG4GyCOcVUc9DBnfHe7T6seXz99XV836-uu31cW6sZKxqdHcuU4K53jrOBNb55h0HJA7IjsGcqsUBwdSiZZK0lJqCbMKqVQaW0EpP60-PXr3Kf6eMU9m9NniMEDAOGfTSdW2RLG2kB8fyV8woPGhj1MCe0-bCyY7qTUXvFCLF6hyHI7exoC9L_2XBmyKOSfszT75EdJfQ4m5j888xGe0MNSU-MrAh8OP5-2I7gn_n1cBzg8AZAtDnyBYn5853glOyl7-Ad-3oMY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>856770627</pqid></control><display><type>article</type><title>Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma</title><source>Oxford University Press Journals</source><source>MEDLINE</source><creator>LARGER MANFIO, Josélia ; SANTOS, Verônica Jorge ; LANCHOTE, Vera Lúcia ; SANTOS, Luciana M ; CARMONA, Maria José C ; AULER, José Otavio ; BRUM JUNIOR, Liberato ; DONADUZZI, Carmen Maria ; SANTOS, Silvia R. C. J</creator><creatorcontrib>LARGER MANFIO, Josélia ; SANTOS, Verônica Jorge ; LANCHOTE, Vera Lúcia ; SANTOS, Luciana M ; CARMONA, Maria José C ; AULER, José Otavio ; BRUM JUNIOR, Liberato ; DONADUZZI, Carmen Maria ; SANTOS, Silvia R. C. J</creatorcontrib><description>A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.1093/jaoac/94.1.136</identifier><identifier>PMID: 21391490</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>Analgesics, Opioid - blood ; Analgesics, Opioid - standards ; Biological and medical sciences ; Blood Chemical Analysis - methods ; Blood Chemical Analysis - standards ; Blood Chemical Analysis - statistics & numerical data ; Blood plasma ; Cardiac Surgical Procedures ; Chemical properties ; Chromatography, High Pressure Liquid - methods ; Chromatography, High Pressure Liquid - statistics & numerical data ; Fentanyl - blood ; Fentanyl - standards ; Food industries ; Fundamental and applied biological sciences. Psychology ; High performance liquid chromatography ; Humans ; Identification and classification ; Mass spectrometry ; Methods ; Morphine ; Morphine - blood ; Morphine - standards ; Reference Standards ; Spectrometry, Mass, Electrospray Ionization - methods ; Sufentanil ; Sufentanil - blood ; Sufentanil - standards ; Tandem Mass Spectrometry - methods ; Tandem Mass Spectrometry - statistics & numerical data</subject><ispartof>Journal of AOAC International, 2011, Vol.94 (1), p.136-142</ispartof><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2011 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c522t-93dd854dd37d324bdd25d3ae3d0582a5b663ada5647150711c02c6e1569e74113</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23843066$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21391490$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LARGER MANFIO, Josélia</creatorcontrib><creatorcontrib>SANTOS, Verônica Jorge</creatorcontrib><creatorcontrib>LANCHOTE, Vera Lúcia</creatorcontrib><creatorcontrib>SANTOS, Luciana M</creatorcontrib><creatorcontrib>CARMONA, Maria José C</creatorcontrib><creatorcontrib>AULER, José Otavio</creatorcontrib><creatorcontrib>BRUM JUNIOR, Liberato</creatorcontrib><creatorcontrib>DONADUZZI, Carmen Maria</creatorcontrib><creatorcontrib>SANTOS, Silvia R. C. J</creatorcontrib><title>Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery.</description><subject>Analgesics, Opioid - blood</subject><subject>Analgesics, Opioid - standards</subject><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis - methods</subject><subject>Blood Chemical Analysis - standards</subject><subject>Blood Chemical Analysis - statistics & numerical data</subject><subject>Blood plasma</subject><subject>Cardiac Surgical Procedures</subject><subject>Chemical properties</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, High Pressure Liquid - statistics & numerical data</subject><subject>Fentanyl - blood</subject><subject>Fentanyl - standards</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Morphine</subject><subject>Morphine - blood</subject><subject>Morphine - standards</subject><subject>Reference Standards</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Sufentanil</subject><subject>Sufentanil - blood</subject><subject>Sufentanil - standards</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tandem Mass Spectrometry - statistics & numerical data</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkd9rFDEQxxex2Nr66qMsSPFp7_J7N4_lqla4o4Wqr2EumfVSdpMz2RX8703bs30pGUgYPvMhzLeq3lOyoETz5R1EsEstFnRBuXpVnVAtRNNqxl6XN1Gk4aylx9XbnO8IEVQR9qY6ZpRrKjQ5qfIl_sEh7kcMUw3B1T9h8A4mH0Md-9Kpr27Wq-XmtlS9wWkXXd3HVE87rC9xwjT68ETfzn3RQPDDg2oT037nA9a-WOaxuG4GyCOcVUc9DBnfHe7T6seXz99XV836-uu31cW6sZKxqdHcuU4K53jrOBNb55h0HJA7IjsGcqsUBwdSiZZK0lJqCbMKqVQaW0EpP60-PXr3Kf6eMU9m9NniMEDAOGfTSdW2RLG2kB8fyV8woPGhj1MCe0-bCyY7qTUXvFCLF6hyHI7exoC9L_2XBmyKOSfszT75EdJfQ4m5j888xGe0MNSU-MrAh8OP5-2I7gn_n1cBzg8AZAtDnyBYn5853glOyl7-Ad-3oMY</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>LARGER MANFIO, Josélia</creator><creator>SANTOS, Verônica Jorge</creator><creator>LANCHOTE, Vera Lúcia</creator><creator>SANTOS, Luciana M</creator><creator>CARMONA, Maria José C</creator><creator>AULER, José Otavio</creator><creator>BRUM JUNIOR, Liberato</creator><creator>DONADUZZI, Carmen Maria</creator><creator>SANTOS, Silvia R. C. J</creator><general>AOAC International</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma</title><author>LARGER MANFIO, Josélia ; SANTOS, Verônica Jorge ; LANCHOTE, Vera Lúcia ; SANTOS, Luciana M ; CARMONA, Maria José C ; AULER, José Otavio ; BRUM JUNIOR, Liberato ; DONADUZZI, Carmen Maria ; SANTOS, Silvia R. C. J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c522t-93dd854dd37d324bdd25d3ae3d0582a5b663ada5647150711c02c6e1569e74113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Analgesics, Opioid - blood</topic><topic>Analgesics, Opioid - standards</topic><topic>Biological and medical sciences</topic><topic>Blood Chemical Analysis - methods</topic><topic>Blood Chemical Analysis - standards</topic><topic>Blood Chemical Analysis - statistics & numerical data</topic><topic>Blood plasma</topic><topic>Cardiac Surgical Procedures</topic><topic>Chemical properties</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, High Pressure Liquid - statistics & numerical data</topic><topic>Fentanyl - blood</topic><topic>Fentanyl - standards</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High performance liquid chromatography</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Morphine</topic><topic>Morphine - blood</topic><topic>Morphine - standards</topic><topic>Reference Standards</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Sufentanil</topic><topic>Sufentanil - blood</topic><topic>Sufentanil - standards</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tandem Mass Spectrometry - statistics & numerical data</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LARGER MANFIO, Josélia</creatorcontrib><creatorcontrib>SANTOS, Verônica Jorge</creatorcontrib><creatorcontrib>LANCHOTE, Vera Lúcia</creatorcontrib><creatorcontrib>SANTOS, Luciana M</creatorcontrib><creatorcontrib>CARMONA, Maria José C</creatorcontrib><creatorcontrib>AULER, José Otavio</creatorcontrib><creatorcontrib>BRUM JUNIOR, Liberato</creatorcontrib><creatorcontrib>DONADUZZI, Carmen Maria</creatorcontrib><creatorcontrib>SANTOS, Silvia R. C. J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of AOAC International</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LARGER MANFIO, Josélia</au><au>SANTOS, Verônica Jorge</au><au>LANCHOTE, Vera Lúcia</au><au>SANTOS, Luciana M</au><au>CARMONA, Maria José C</au><au>AULER, José Otavio</au><au>BRUM JUNIOR, Liberato</au><au>DONADUZZI, Carmen Maria</au><au>SANTOS, Silvia R. C. J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma</atitle><jtitle>Journal of AOAC International</jtitle><addtitle>J AOAC Int</addtitle><date>2011</date><risdate>2011</risdate><volume>94</volume><issue>1</issue><spage>136</spage><epage>142</epage><pages>136-142</pages><issn>1060-3271</issn><eissn>1944-7922</eissn><abstract>A sensitive and fast HPLC/MS/MS method for measurement of sufentanil and morphine in plasma was developed and validated. A single liquid-liquid extraction in alkaline medium was used for the cleanup of plasma, and fentanyl was added as an internal standard (IS). The analyses were carried out using a C18 column and the mobile phase acetonitrile-5 mM ammonium acetate + 0.25% formic acid (70 + 30, v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source in positive mode was set up in the selective reaction monitoring mode to detect precursor --> product ion transition 387.0 > 238.0, 285.7 > 165.1, and 337.0 > 188.0 for sufentanil, morphine, and IS, respectively. The method was linear in the 0.05 (LOQ) - 500 ng/mL range for sufentanil and 10 (LOQ) - 1000 ng/mL range for morphine. Good selectivity, linearity, precision, accuracy, and robustness were obtained for the HPLC/MS/MS method. The proposed method was successfully applied for the determination of sufentanil and morphine in patients undergoing cardiac surgery.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>21391490</pmid><doi>10.1093/jaoac/94.1.136</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1060-3271 |
| ispartof | Journal of AOAC International, 2011, Vol.94 (1), p.136-142 |
| issn | 1060-3271 1944-7922 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_856770627 |
| source | Oxford University Press Journals; MEDLINE |
| subjects | Analgesics, Opioid - blood Analgesics, Opioid - standards Biological and medical sciences Blood Chemical Analysis - methods Blood Chemical Analysis - standards Blood Chemical Analysis - statistics & numerical data Blood plasma Cardiac Surgical Procedures Chemical properties Chromatography, High Pressure Liquid - methods Chromatography, High Pressure Liquid - statistics & numerical data Fentanyl - blood Fentanyl - standards Food industries Fundamental and applied biological sciences. Psychology High performance liquid chromatography Humans Identification and classification Mass spectrometry Methods Morphine Morphine - blood Morphine - standards Reference Standards Spectrometry, Mass, Electrospray Ionization - methods Sufentanil Sufentanil - blood Sufentanil - standards Tandem Mass Spectrometry - methods Tandem Mass Spectrometry - statistics & numerical data |
| title | Development and Validation of an HPLC/MS/MS Method for the Determination of Sufentanil and Morphine in Human Plasma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T08%3A37%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20and%20Validation%20of%20an%20HPLC/MS/MS%20Method%20for%20the%20Determination%20of%20Sufentanil%20and%20Morphine%20in%20Human%20Plasma&rft.jtitle=Journal%20of%20AOAC%20International&rft.au=LARGER%20MANFIO,%20Jos%C3%A9lia&rft.date=2011&rft.volume=94&rft.issue=1&rft.spage=136&rft.epage=142&rft.pages=136-142&rft.issn=1060-3271&rft.eissn=1944-7922&rft_id=info:doi/10.1093/jaoac/94.1.136&rft_dat=%3Cgale_proqu%3EA258599343%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=856770627&rft_id=info:pmid/21391490&rft_galeid=A258599343&rfr_iscdi=true |