Assessing the role of the RND efflux pump in metronidazole resistance of Helicobacter pylori by RT-PCR assay
Metronidazole is a significant antibiotic used for eradication of Helicobacter pylori infections and it is of notice that metronidazole-resistant clinical isolates have been found in high rates worldwide. While the RND family of efflux pumps plays a central role in drug resistance among Gram-negativ...
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| Veröffentlicht in: | Journal of infection in developing countries 2011-02, Vol.5 (2), p.88-93 |
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| container_title | Journal of infection in developing countries |
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| creator | Mehrabadi, Jalil Fallah Sirous, Mehrandokht Daryani, Naser Ebrahimi Eshraghi, Saeed Akbari, Bahram Shirazi, Mohammad Hasan |
| description | Metronidazole is a significant antibiotic used for eradication of Helicobacter pylori infections and it is of notice that metronidazole-resistant clinical isolates have been found in high rates worldwide. While the RND family of efflux pumps plays a central role in drug resistance among Gram-negative bacteria, this is questionable for H. pylori.
To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.
The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.
Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. Furthermore, the RND family of efflux pumps may contribute to metronidazole resistance in clinical isolates of H. pylori. |
| doi_str_mv | 10.3855/jidc.1187 |
| format | Article |
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To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.
The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.
Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. Furthermore, the RND family of efflux pumps may contribute to metronidazole resistance in clinical isolates of H. pylori.</description><identifier>ISSN: 1972-2680</identifier><identifier>ISSN: 2036-6590</identifier><identifier>EISSN: 1972-2680</identifier><identifier>DOI: 10.3855/jidc.1187</identifier><identifier>PMID: 21389587</identifier><language>eng</language><publisher>Italy: Journal of Infection in Developing Countries</publisher><subject>Anti-Bacterial Agents - pharmacology ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Outer Membrane Proteins - metabolism ; Drug Resistance, Bacterial ; Helicobacter Infections - microbiology ; Helicobacter pylori - drug effects ; Helicobacter pylori - genetics ; Helicobacter pylori - growth & development ; Helicobacter pylori - metabolism ; Humans ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Metronidazole - metabolism ; Metronidazole - pharmacology ; Microbial Sensitivity Tests ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription, Genetic</subject><ispartof>Journal of infection in developing countries, 2011-02, Vol.5 (2), p.88-93</ispartof><rights>2011. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-7b8685cf2f2f884fb296be05489824abaa977680236d31a4d1dec4425aa0a2a73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21389587$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mehrabadi, Jalil Fallah</creatorcontrib><creatorcontrib>Sirous, Mehrandokht</creatorcontrib><creatorcontrib>Daryani, Naser Ebrahimi</creatorcontrib><creatorcontrib>Eshraghi, Saeed</creatorcontrib><creatorcontrib>Akbari, Bahram</creatorcontrib><creatorcontrib>Shirazi, Mohammad Hasan</creatorcontrib><title>Assessing the role of the RND efflux pump in metronidazole resistance of Helicobacter pylori by RT-PCR assay</title><title>Journal of infection in developing countries</title><addtitle>J Infect Dev Ctries</addtitle><description>Metronidazole is a significant antibiotic used for eradication of Helicobacter pylori infections and it is of notice that metronidazole-resistant clinical isolates have been found in high rates worldwide. While the RND family of efflux pumps plays a central role in drug resistance among Gram-negative bacteria, this is questionable for H. pylori.
To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.
The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.
Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. 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While the RND family of efflux pumps plays a central role in drug resistance among Gram-negative bacteria, this is questionable for H. pylori.
To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.
The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.
Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. Furthermore, the RND family of efflux pumps may contribute to metronidazole resistance in clinical isolates of H. pylori.</abstract><cop>Italy</cop><pub>Journal of Infection in Developing Countries</pub><pmid>21389587</pmid><doi>10.3855/jidc.1187</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of infection in developing countries, 2011-02, Vol.5 (2), p.88-93 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Anti-Bacterial Agents - pharmacology Bacterial Outer Membrane Proteins - genetics Bacterial Outer Membrane Proteins - metabolism Drug Resistance, Bacterial Helicobacter Infections - microbiology Helicobacter pylori - drug effects Helicobacter pylori - genetics Helicobacter pylori - growth & development Helicobacter pylori - metabolism Humans Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Metronidazole - metabolism Metronidazole - pharmacology Microbial Sensitivity Tests Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic |
| title | Assessing the role of the RND efflux pump in metronidazole resistance of Helicobacter pylori by RT-PCR assay |
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