Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae)
Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S...
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| Veröffentlicht in: | Current microbiology 2009-09, Vol.59 (3), p.295-301 |
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| creator | Coscrato, Virginia E Braz, Antônio S. K Perondini, André L. P Selivon, Denise Marino, Celso L |
| description | Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S rDNA, ftsZ, and wsp) in conjunction with the polymerase chain reaction (PCR), cloning and sequencing to study the infection of fruit flies (Anastrepha spp. and Ceratitis capitata) by Wolbachia. The flies were collected at several localities in Brazil and at Guayaquil, Ecuador. All of the fruit flies studied were infected with Wolbachia supergroup A, in agreement with the high prevalence of this group in South America. Phylogenetic analysis showed that the wsp gene was the most sensitive gene for studying the relationships among Wolbachia strains. The Wolbachia sequences detected in these fruit flies were similar to those such as wMel reported for other fruit flies. These results show that the infection of Anastrepha fruit flies by Wolbachia is much more widespread than previously thought. |
| doi_str_mv | 10.1007/s00284-009-9433-8 |
| format | Article |
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K ; Perondini, André L. P ; Selivon, Denise ; Marino, Celso L</creator><creatorcontrib>Coscrato, Virginia E ; Braz, Antônio S. K ; Perondini, André L. P ; Selivon, Denise ; Marino, Celso L</creatorcontrib><description>Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S rDNA, ftsZ, and wsp) in conjunction with the polymerase chain reaction (PCR), cloning and sequencing to study the infection of fruit flies (Anastrepha spp. and Ceratitis capitata) by Wolbachia. The flies were collected at several localities in Brazil and at Guayaquil, Ecuador. All of the fruit flies studied were infected with Wolbachia supergroup A, in agreement with the high prevalence of this group in South America. Phylogenetic analysis showed that the wsp gene was the most sensitive gene for studying the relationships among Wolbachia strains. The Wolbachia sequences detected in these fruit flies were similar to those such as wMel reported for other fruit flies. These results show that the infection of Anastrepha fruit flies by Wolbachia is much more widespread than previously thought.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-009-9433-8</identifier><identifier>PMID: 19536597</identifier><language>eng</language><publisher>New York: New York : Springer-Verlag</publisher><subject>Anastrepha ; Animals ; Arthropoda ; Bacteria ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Proteins - genetics ; Biomedical and Life Sciences ; Biotechnology ; Brazil ; Ceratitis capitata ; Cloning ; Cluster Analysis ; Cytoskeletal Proteins - genetics ; Diptera ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Ecuador ; Female ; Fruits ; Infections ; Insects ; Life Sciences ; Microbiology ; Molecular Sequence Data ; Phylogeny ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Studies ; Tephritidae ; Tephritidae - microbiology ; Wolbachia ; Wolbachia - classification ; Wolbachia - genetics ; Wolbachia - isolation & purification</subject><ispartof>Current microbiology, 2009-09, Vol.59 (3), p.295-301</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-b7ee3c67180d691e64e13d6e86bbded445139bce59316f36a13255aa3cb19e753</citedby><cites>FETCH-LOGICAL-c425t-b7ee3c67180d691e64e13d6e86bbded445139bce59316f36a13255aa3cb19e753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-009-9433-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-009-9433-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19536597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Coscrato, Virginia E</creatorcontrib><creatorcontrib>Braz, Antônio S. K</creatorcontrib><creatorcontrib>Perondini, André L. P</creatorcontrib><creatorcontrib>Selivon, Denise</creatorcontrib><creatorcontrib>Marino, Celso L</creatorcontrib><title>Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae)</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S rDNA, ftsZ, and wsp) in conjunction with the polymerase chain reaction (PCR), cloning and sequencing to study the infection of fruit flies (Anastrepha spp. and Ceratitis capitata) by Wolbachia. The flies were collected at several localities in Brazil and at Guayaquil, Ecuador. All of the fruit flies studied were infected with Wolbachia supergroup A, in agreement with the high prevalence of this group in South America. Phylogenetic analysis showed that the wsp gene was the most sensitive gene for studying the relationships among Wolbachia strains. The Wolbachia sequences detected in these fruit flies were similar to those such as wMel reported for other fruit flies. These results show that the infection of Anastrepha fruit flies by Wolbachia is much more widespread than previously thought.</description><subject>Anastrepha</subject><subject>Animals</subject><subject>Arthropoda</subject><subject>Bacteria</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacterial Proteins - genetics</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brazil</subject><subject>Ceratitis capitata</subject><subject>Cloning</subject><subject>Cluster Analysis</subject><subject>Cytoskeletal Proteins - genetics</subject><subject>Diptera</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Ecuador</subject><subject>Female</subject><subject>Fruits</subject><subject>Infections</subject><subject>Insects</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phylogeny</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Studies</subject><subject>Tephritidae</subject><subject>Tephritidae - microbiology</subject><subject>Wolbachia</subject><subject>Wolbachia - classification</subject><subject>Wolbachia - genetics</subject><subject>Wolbachia - isolation & purification</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kE1P3DAQhq2KCraUH8AFIg5tOaSd8WfMDdEuVELqoaAeLSeZBaNsstjJgX-PV1kJqQdOI3meeWf8MHaM8B0BzI8EwCtZAtjSSiHK6gNboBS8BGtxjy1AyPyoFR6wTyk9ASC3gPvsAK0SWlmzYBf_hq72zWPwReiLy96nMdLm0RfLOIWxWHaBUvHtZ9iMFP1FcZd7MYyh9XT-mX1c-S7R0a4esvvlr7urm_L2z_Xvq8vbspFcjWVtiESjDVbQaoukJaFoNVW6rltqpVQobN2QsgL1SmiPgivlvWhqtGSUOGRf59xNHJ4nSqNbh9RQ1_mehim5SmmjhcUqk1_eJbVRBgBEBs_-A5-GKfb5F46D4hw48gzhDDVxSCnSym1iWPv44hDc1r-b_bvs3239u-0FJ7vgqV5T-zaxE54BPgMpt_oHim-b30s9nYdWfnD-IYbk7v9yQAGos1hpxCt8ZpZ4</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Coscrato, Virginia E</creator><creator>Braz, Antônio S. 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P</creator><creator>Selivon, Denise</creator><creator>Marino, Celso L</creator><general>New York : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7SS</scope></search><sort><creationdate>20090901</creationdate><title>Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae)</title><author>Coscrato, Virginia E ; Braz, Antônio S. K ; Perondini, André L. P ; Selivon, Denise ; Marino, Celso L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-b7ee3c67180d691e64e13d6e86bbded445139bce59316f36a13255aa3cb19e753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anastrepha</topic><topic>Animals</topic><topic>Arthropoda</topic><topic>Bacteria</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacterial Proteins - genetics</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Brazil</topic><topic>Ceratitis capitata</topic><topic>Cloning</topic><topic>Cluster Analysis</topic><topic>Cytoskeletal Proteins - genetics</topic><topic>Diptera</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Ecuador</topic><topic>Female</topic><topic>Fruits</topic><topic>Infections</topic><topic>Insects</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phylogeny</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Studies</topic><topic>Tephritidae</topic><topic>Tephritidae - microbiology</topic><topic>Wolbachia</topic><topic>Wolbachia - classification</topic><topic>Wolbachia - genetics</topic><topic>Wolbachia - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coscrato, Virginia E</creatorcontrib><creatorcontrib>Braz, Antônio S. 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K</au><au>Perondini, André L. P</au><au>Selivon, Denise</au><au>Marino, Celso L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae)</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>59</volume><issue>3</issue><spage>295</spage><epage>301</epage><pages>295-301</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>Endosymbiotic bacteria of the genus Wolbachia are widespread among arthropods and cause a variety of reproductive abnormalities, such as cytoplasmic incompatibility, thelytokous parthenogenesis, male-killing, and host feminization. In this study, we used three sets of Wolbachia-specific primers (16S rDNA, ftsZ, and wsp) in conjunction with the polymerase chain reaction (PCR), cloning and sequencing to study the infection of fruit flies (Anastrepha spp. and Ceratitis capitata) by Wolbachia. The flies were collected at several localities in Brazil and at Guayaquil, Ecuador. All of the fruit flies studied were infected with Wolbachia supergroup A, in agreement with the high prevalence of this group in South America. Phylogenetic analysis showed that the wsp gene was the most sensitive gene for studying the relationships among Wolbachia strains. The Wolbachia sequences detected in these fruit flies were similar to those such as wMel reported for other fruit flies. These results show that the infection of Anastrepha fruit flies by Wolbachia is much more widespread than previously thought.</abstract><cop>New York</cop><pub>New York : Springer-Verlag</pub><pmid>19536597</pmid><doi>10.1007/s00284-009-9433-8</doi><tpages>7</tpages></addata></record> |
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| subjects | Anastrepha Animals Arthropoda Bacteria Bacterial Outer Membrane Proteins - genetics Bacterial Proteins - genetics Biomedical and Life Sciences Biotechnology Brazil Ceratitis capitata Cloning Cluster Analysis Cytoskeletal Proteins - genetics Diptera DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Ecuador Female Fruits Infections Insects Life Sciences Microbiology Molecular Sequence Data Phylogeny RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Studies Tephritidae Tephritidae - microbiology Wolbachia Wolbachia - classification Wolbachia - genetics Wolbachia - isolation & purification |
| title | Wolbachia in Anastrepha Fruit Flies (Diptera: Tephritidae) |
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