Facilitation at single synapses probed with optical quantal analysis
Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient inc...
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| Veröffentlicht in: | Nature neuroscience 2002-07, Vol.5 (7), p.657-664 |
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| creator | Oertner, Thomas G. Sabatini, Bernardo L. Nimchinsky, Esther A. Svoboda, Karel |
| description | Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient increases in [Ca
2+
] mediated by synaptic
N
-methyl-
D
-aspartate receptors (NMDARs) in individual dendritic spines of CA1 pyramidal neurons in rat brain slices, enabling quantal analysis at single synapses. We found that changes in release probability, produced by paired-pulse facilitation (PPF) or by manipulation of presynaptic adenosine receptors, were associated with changes in glutamate concentration in the synaptic cleft, indicating that single synapses can release a variable amount of glutamate per action potential. The relationship between release probability and response size is consistent with a binomial model of vesicle release with several (>5) independent release sites per active zone, suggesting that multivesicular release contributes to facilitation at these synapses. |
| doi_str_mv | 10.1038/nn867 |
| format | Article |
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2+
] mediated by synaptic
N
-methyl-
D
-aspartate receptors (NMDARs) in individual dendritic spines of CA1 pyramidal neurons in rat brain slices, enabling quantal analysis at single synapses. We found that changes in release probability, produced by paired-pulse facilitation (PPF) or by manipulation of presynaptic adenosine receptors, were associated with changes in glutamate concentration in the synaptic cleft, indicating that single synapses can release a variable amount of glutamate per action potential. The relationship between release probability and response size is consistent with a binomial model of vesicle release with several (>5) independent release sites per active zone, suggesting that multivesicular release contributes to facilitation at these synapses.</description><identifier>ISSN: 1097-6256</identifier><identifier>EISSN: 1546-1726</identifier><identifier>DOI: 10.1038/nn867</identifier><identifier>PMID: 12055631</identifier><identifier>CODEN: NANEFN</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>2-Chloroadenosine - pharmacology ; Action Potentials - physiology ; Animal Genetics and Genomics ; Animals ; Behavioral Sciences ; Biological Techniques ; Biomedical and Life Sciences ; Biomedicine ; Calcium - analysis ; Calcium - metabolism ; Calcium Signaling - drug effects ; Calcium Signaling - physiology ; Electric Stimulation ; Excitatory Postsynaptic Potentials - physiology ; Glutamic Acid - metabolism ; In Vitro Techniques ; Neural transmission ; Neurobiology ; Neuronal Plasticity - physiology ; Neuroplasticity ; Neurosciences ; Neurotransmitter Agents - metabolism ; Neurotransmitter Agents - secretion ; Physiological aspects ; Presynaptic Terminals - metabolism ; Pyramidal Cells - drug effects ; Pyramidal Cells - physiology ; Pyramidal Cells - ultrastructure ; Rats ; Receptors, N-Methyl-D-Aspartate - metabolism ; Receptors, Purinergic P1 - metabolism ; Sensory Thresholds - physiology ; Synapses ; Synapses - drug effects ; Synapses - physiology ; Synapses - secretion</subject><ispartof>Nature neuroscience, 2002-07, Vol.5 (7), p.657-664</ispartof><rights>Springer Nature America, Inc. 2002</rights><rights>COPYRIGHT 2002 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Jul 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c467t-4e4ec4321334e87cb7501f32dbf42902fd280637da76969977c6648bd17f59a3</citedby><cites>FETCH-LOGICAL-c467t-4e4ec4321334e87cb7501f32dbf42902fd280637da76969977c6648bd17f59a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nn867$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nn867$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12055631$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oertner, Thomas G.</creatorcontrib><creatorcontrib>Sabatini, Bernardo L.</creatorcontrib><creatorcontrib>Nimchinsky, Esther A.</creatorcontrib><creatorcontrib>Svoboda, Karel</creatorcontrib><title>Facilitation at single synapses probed with optical quantal analysis</title><title>Nature neuroscience</title><addtitle>Nat Neurosci</addtitle><addtitle>Nat Neurosci</addtitle><description>Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient increases in [Ca
2+
] mediated by synaptic
N
-methyl-
D
-aspartate receptors (NMDARs) in individual dendritic spines of CA1 pyramidal neurons in rat brain slices, enabling quantal analysis at single synapses. We found that changes in release probability, produced by paired-pulse facilitation (PPF) or by manipulation of presynaptic adenosine receptors, were associated with changes in glutamate concentration in the synaptic cleft, indicating that single synapses can release a variable amount of glutamate per action potential. The relationship between release probability and response size is consistent with a binomial model of vesicle release with several (>5) independent release sites per active zone, suggesting that multivesicular release contributes to facilitation at these synapses.</description><subject>2-Chloroadenosine - pharmacology</subject><subject>Action Potentials - physiology</subject><subject>Animal Genetics and Genomics</subject><subject>Animals</subject><subject>Behavioral Sciences</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Calcium - analysis</subject><subject>Calcium - metabolism</subject><subject>Calcium Signaling - drug effects</subject><subject>Calcium Signaling - physiology</subject><subject>Electric Stimulation</subject><subject>Excitatory Postsynaptic Potentials - physiology</subject><subject>Glutamic Acid - metabolism</subject><subject>In Vitro Techniques</subject><subject>Neural transmission</subject><subject>Neurobiology</subject><subject>Neuronal Plasticity - physiology</subject><subject>Neuroplasticity</subject><subject>Neurosciences</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Neurotransmitter Agents - secretion</subject><subject>Physiological aspects</subject><subject>Presynaptic Terminals - metabolism</subject><subject>Pyramidal Cells - drug effects</subject><subject>Pyramidal Cells - physiology</subject><subject>Pyramidal Cells - ultrastructure</subject><subject>Rats</subject><subject>Receptors, N-Methyl-D-Aspartate - metabolism</subject><subject>Receptors, Purinergic P1 - metabolism</subject><subject>Sensory Thresholds - physiology</subject><subject>Synapses</subject><subject>Synapses - drug effects</subject><subject>Synapses - physiology</subject><subject>Synapses - secretion</subject><issn>1097-6256</issn><issn>1546-1726</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkt9rFDEQx4NYbD37L8giWPFha35Oso-lWi0UCtr3kM1mz5S97HUni95_35x3cJwPyjxMSD7znfmSIeSc0UtGhfmUkgH9gpwxJaFmmsPLcqaNroErOCWvER8ppVqZ5hU5ZZwqBYKdkc83zschZpfjmCqXK4xpOYQKN8mtMWC1nsY2dNWvmH9W4zpH74bqaXYpl-ySGzYY8Q056d2A4XyfF-Th5svD9bf67v7r7fXVXe0l6FzLIIOXgjMhZDDat1pR1gvetb3kDeV9xw0FoTunoYGm0doDSNN2TPeqcWJBPuxky0xPc8BsVxF9GAaXwjijNQo0iKJRyIt_kpoZpbiA_4LMSBCqxIK8-wt8HOep-EfLtdSNkmqrdrmDlm4INqZ-zJPzJbqwin5MoY_l_mrbHJgBWQo-HhUUJoffeelmRHv74_sx-37H-mlEnEJv11NcuWljGbXbFbB_VqBwb_eTzu0qdAdq_-cHz1ie0jJMByvHSs8MkLUC</recordid><startdate>20020701</startdate><enddate>20020701</enddate><creator>Oertner, Thomas G.</creator><creator>Sabatini, Bernardo L.</creator><creator>Nimchinsky, Esther A.</creator><creator>Svoboda, Karel</creator><general>Nature Publishing Group US</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20020701</creationdate><title>Facilitation at single synapses probed with optical quantal analysis</title><author>Oertner, Thomas G. ; Sabatini, Bernardo L. ; Nimchinsky, Esther A. ; Svoboda, Karel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c467t-4e4ec4321334e87cb7501f32dbf42902fd280637da76969977c6648bd17f59a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>2-Chloroadenosine - pharmacology</topic><topic>Action Potentials - physiology</topic><topic>Animal Genetics and Genomics</topic><topic>Animals</topic><topic>Behavioral Sciences</topic><topic>Biological Techniques</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Calcium - analysis</topic><topic>Calcium - metabolism</topic><topic>Calcium Signaling - drug effects</topic><topic>Calcium Signaling - physiology</topic><topic>Electric Stimulation</topic><topic>Excitatory Postsynaptic Potentials - physiology</topic><topic>Glutamic Acid - metabolism</topic><topic>In Vitro Techniques</topic><topic>Neural transmission</topic><topic>Neurobiology</topic><topic>Neuronal Plasticity - physiology</topic><topic>Neuroplasticity</topic><topic>Neurosciences</topic><topic>Neurotransmitter Agents - metabolism</topic><topic>Neurotransmitter Agents - secretion</topic><topic>Physiological aspects</topic><topic>Presynaptic Terminals - metabolism</topic><topic>Pyramidal Cells - drug effects</topic><topic>Pyramidal Cells - 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Academic</collection><jtitle>Nature neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oertner, Thomas G.</au><au>Sabatini, Bernardo L.</au><au>Nimchinsky, Esther A.</au><au>Svoboda, Karel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Facilitation at single synapses probed with optical quantal analysis</atitle><jtitle>Nature neuroscience</jtitle><stitle>Nat Neurosci</stitle><addtitle>Nat Neurosci</addtitle><date>2002-07-01</date><risdate>2002</risdate><volume>5</volume><issue>7</issue><spage>657</spage><epage>664</epage><pages>657-664</pages><issn>1097-6256</issn><eissn>1546-1726</eissn><coden>NANEFN</coden><abstract>Many synapses can change their strength rapidly in a use-dependent manner, but the mechanisms of such short-term plasticity remain unknown. To understand these mechanisms, measurements of neurotransmitter release at single synapses are required. We probed transmitter release by imaging transient increases in [Ca
2+
] mediated by synaptic
N
-methyl-
D
-aspartate receptors (NMDARs) in individual dendritic spines of CA1 pyramidal neurons in rat brain slices, enabling quantal analysis at single synapses. We found that changes in release probability, produced by paired-pulse facilitation (PPF) or by manipulation of presynaptic adenosine receptors, were associated with changes in glutamate concentration in the synaptic cleft, indicating that single synapses can release a variable amount of glutamate per action potential. The relationship between release probability and response size is consistent with a binomial model of vesicle release with several (>5) independent release sites per active zone, suggesting that multivesicular release contributes to facilitation at these synapses.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>12055631</pmid><doi>10.1038/nn867</doi><tpages>8</tpages></addata></record> |
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| subjects | 2-Chloroadenosine - pharmacology Action Potentials - physiology Animal Genetics and Genomics Animals Behavioral Sciences Biological Techniques Biomedical and Life Sciences Biomedicine Calcium - analysis Calcium - metabolism Calcium Signaling - drug effects Calcium Signaling - physiology Electric Stimulation Excitatory Postsynaptic Potentials - physiology Glutamic Acid - metabolism In Vitro Techniques Neural transmission Neurobiology Neuronal Plasticity - physiology Neuroplasticity Neurosciences Neurotransmitter Agents - metabolism Neurotransmitter Agents - secretion Physiological aspects Presynaptic Terminals - metabolism Pyramidal Cells - drug effects Pyramidal Cells - physiology Pyramidal Cells - ultrastructure Rats Receptors, N-Methyl-D-Aspartate - metabolism Receptors, Purinergic P1 - metabolism Sensory Thresholds - physiology Synapses Synapses - drug effects Synapses - physiology Synapses - secretion |
| title | Facilitation at single synapses probed with optical quantal analysis |
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