Identification of Sef, a novel modulator of FGF signalling
Fibroblast growth factors (FGFs) are members of a family of some 30 secreted proteins important in the regulation of cellular proliferation, migration, differentiation and survival. Here we report the identification of a novel modulator of FGF signal transduction, sef, isolated from a zebrafish embr...
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| Veröffentlicht in: | Nature cell biology 2002-02, Vol.4 (2), p.165-169 |
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| creator | Dawid, Igor B Tsang, Michael Friesel, Robert Kudoh, Tetsuhiro |
| description | Fibroblast growth factors (FGFs) are members of a family of some 30 secreted proteins important in the regulation of cellular proliferation, migration, differentiation and survival. Here we report the identification of a novel modulator of FGF signal transduction, sef, isolated from a zebrafish embryo library through an in situ hybridization screen. The sef gene encodes a transmembrane protein, and belongs to the synexpression group that includes some of the fgf genes. Sef expression is positively regulated by FGF, and ectopic expression of sef in zebrafish or Xenopus laevis embryos specifically inhibits FGF signalling. In co-immunoprecipitation assays, the intracellular domain of Sef interacts with FGF receptors, FGFR1 and FGFR2. Injection of antisense sef morpholino oligos mimicked the phenotypes observed by ectopic fgf8 expression, suggesting that Sef is required to limit FGF signalling during development. |
| doi_str_mv | 10.1038/ncb749 |
| format | Article |
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Here we report the identification of a novel modulator of FGF signal transduction, sef, isolated from a zebrafish embryo library through an in situ hybridization screen. The sef gene encodes a transmembrane protein, and belongs to the synexpression group that includes some of the fgf genes. Sef expression is positively regulated by FGF, and ectopic expression of sef in zebrafish or Xenopus laevis embryos specifically inhibits FGF signalling. In co-immunoprecipitation assays, the intracellular domain of Sef interacts with FGF receptors, FGFR1 and FGFR2. Injection of antisense sef morpholino oligos mimicked the phenotypes observed by ectopic fgf8 expression, suggesting that Sef is required to limit FGF signalling during development.</description><identifier>ISSN: 1465-7392</identifier><identifier>ISSN: 1476-4679</identifier><identifier>EISSN: 1476-4679</identifier><identifier>DOI: 10.1038/ncb749</identifier><identifier>PMID: 11802164</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Animals ; Biomedical and Life Sciences ; brief-communication ; Cancer Research ; Cell Biology ; Danio rerio ; Developmental Biology ; Embryo, Nonmammalian - metabolism ; Embryos ; Fibroblast growth factors ; Fibroblast Growth Factors - genetics ; Fibroblast Growth Factors - metabolism ; Freshwater ; Gene expression ; Genes ; Growth factors ; Hybridization ; In Situ Hybridization ; Injection ; Life Sciences ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; Molecular Sequence Data ; Oligonucleotides, Antisense - genetics ; Oligonucleotides, Antisense - metabolism ; Phosphorylation ; Physiological aspects ; Receptors, Fibroblast Growth Factor - metabolism ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Signal transduction ; Signal Transduction - physiology ; Stem Cells ; Tissue Transplantation ; Xenopus laevis ; Xenopus laevis - physiology ; Zebrafish - physiology</subject><ispartof>Nature cell biology, 2002-02, Vol.4 (2), p.165-169</ispartof><rights>Springer Nature Limited 2002</rights><rights>COPYRIGHT 2002 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group Feb 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-cacb9bc7ccdabf7919adaf6a559319faaf875d6d99c45a66d89b0b146067d7473</citedby><cites>FETCH-LOGICAL-c491t-cacb9bc7ccdabf7919adaf6a559319faaf875d6d99c45a66d89b0b146067d7473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/ncb749$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/ncb749$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2727,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11802164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dawid, Igor B</creatorcontrib><creatorcontrib>Tsang, Michael</creatorcontrib><creatorcontrib>Friesel, Robert</creatorcontrib><creatorcontrib>Kudoh, Tetsuhiro</creatorcontrib><title>Identification of Sef, a novel modulator of FGF signalling</title><title>Nature cell biology</title><addtitle>Nat Cell Biol</addtitle><addtitle>Nat Cell Biol</addtitle><description>Fibroblast growth factors (FGFs) are members of a family of some 30 secreted proteins important in the regulation of cellular proliferation, migration, differentiation and survival. Here we report the identification of a novel modulator of FGF signal transduction, sef, isolated from a zebrafish embryo library through an in situ hybridization screen. The sef gene encodes a transmembrane protein, and belongs to the synexpression group that includes some of the fgf genes. Sef expression is positively regulated by FGF, and ectopic expression of sef in zebrafish or Xenopus laevis embryos specifically inhibits FGF signalling. In co-immunoprecipitation assays, the intracellular domain of Sef interacts with FGF receptors, FGFR1 and FGFR2. Injection of antisense sef morpholino oligos mimicked the phenotypes observed by ectopic fgf8 expression, suggesting that Sef is required to limit FGF signalling during development.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>brief-communication</subject><subject>Cancer Research</subject><subject>Cell Biology</subject><subject>Danio rerio</subject><subject>Developmental Biology</subject><subject>Embryo, Nonmammalian - metabolism</subject><subject>Embryos</subject><subject>Fibroblast growth factors</subject><subject>Fibroblast Growth Factors - genetics</subject><subject>Fibroblast Growth Factors - metabolism</subject><subject>Freshwater</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Growth factors</subject><subject>Hybridization</subject><subject>In Situ Hybridization</subject><subject>Injection</subject><subject>Life Sciences</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - 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| subjects | Animals Biomedical and Life Sciences brief-communication Cancer Research Cell Biology Danio rerio Developmental Biology Embryo, Nonmammalian - metabolism Embryos Fibroblast growth factors Fibroblast Growth Factors - genetics Fibroblast Growth Factors - metabolism Freshwater Gene expression Genes Growth factors Hybridization In Situ Hybridization Injection Life Sciences Membrane Proteins - genetics Membrane Proteins - metabolism Molecular Sequence Data Oligonucleotides, Antisense - genetics Oligonucleotides, Antisense - metabolism Phosphorylation Physiological aspects Receptors, Fibroblast Growth Factor - metabolism Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Signal transduction Signal Transduction - physiology Stem Cells Tissue Transplantation Xenopus laevis Xenopus laevis - physiology Zebrafish - physiology |
| title | Identification of Sef, a novel modulator of FGF signalling |
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