Targeting of non-lg sequences in place of the V segment by somatic hyper mutation
Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V K segment can be replaced by human β-globin or prokaryotic neo or gpt genes without aff...
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| Veröffentlicht in: | Nature (London) 1995-07, Vol.376 (6537), p.225-229 |
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| container_title | Nature (London) |
| container_volume | 376 |
| creator | Yélamos, J. Klix, N. Goyenechea, B. Lozano, F. Chui, Y. L. FernÃndez, A. GonzÃlez Pannell, R. Neuberger, M. S. Milstein, C. |
| description | Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V
K
segment can be replaced by human β-globin or prokaryotic
neo
or
gpt
genes without affecting the rate of hypermutation; the
V
gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes
in vivo
could find more general applications in biology. |
| doi_str_mv | 10.1038/376225a0 |
| format | Article |
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K
segment can be replaced by human β-globin or prokaryotic
neo
or
gpt
genes without affecting the rate of hypermutation; the
V
gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes
in vivo
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K
segment can be replaced by human β-globin or prokaryotic
neo
or
gpt
genes without affecting the rate of hypermutation; the
V
gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes
in vivo
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K
segment can be replaced by human β-globin or prokaryotic
neo
or
gpt
genes without affecting the rate of hypermutation; the
V
gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes
in vivo
could find more general applications in biology.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1038/376225a0</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1995-07, Vol.376 (6537), p.225-229 |
| issn | 0028-0836 1476-4687 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_856762393 |
| source | Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Humanities and Social Sciences multidisciplinary Science Science (multidisciplinary) |
| title | Targeting of non-lg sequences in place of the V segment by somatic hyper mutation |
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