Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene
In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequenc...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular biochemistry 2002-10, Vol.239 (1-2), p.173-180 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 180 |
|---|---|
| container_issue | 1-2 |
| container_start_page | 173 |
| container_title | Molecular and cellular biochemistry |
| container_volume | 239 |
| creator | Wu, Qiwei Chang, Weihua Rickers-Haunerland, Jutta Higo, Tobi Haunerland, Norbert H |
| description | In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequences analyzed for potential regulatory elements. Comparison with other muscle FABP promoters revealed the presence of a 19-bp imperfect inverted repeat sequence that contains two hexanucleotide half sites (AGTGGT and ATGGGA), interspersed by 3 nucleotides. The promoter activity was studied with reporter gene constructs in L6 myoblasts, in which H-FABP expression is stimulated by long-chain fatty acids in a similar manner as in adult cardiomyocytes. The 19 bp element, located 180 bp upstream of the transcription start site, was found to be essential for the fatty acid induction of gene expression, and gel shift analysis confirmed that this fatty acid response element is capable of binding nuclear proteins both from rat myoblasts and locust muscle in the presence of fatty acids. A similar, but reverse sequence that is present upstream of all mammalian H-FABP promoters may modulate the expression of the rat H-FABP gene. |
| doi_str_mv | 10.1023/A:1020554824176 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_856761513</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>72759698</sourcerecordid><originalsourceid>FETCH-LOGICAL-c312t-4b10f8eda2ff52415bf3d2bc30d1bfbeb178bb29bcf4e4de1ee7fabfc4e5b3673</originalsourceid><addsrcrecordid>eNp9kM1LxDAQxYMouq6evUnwoKdqPpvW27q4KizoQc8lSSdaaZu1SdH1rzfievEgDDzmzY8HbxA6ouScEsYvZpdJiJSiYIKqfAtNqFQ8EyUtt9GEcEKygiq1h_ZDeCWEpqG7aI8yoUpZ8Al6m7_oQdsIQ_OpY-N77B3WuId37HSMa6xtU-MBwsr3ATC00EEfcXzREVvfx8G3IW3p8rFKVNgkfDutt2OIuBuDbQEvZlcP-Bl6OEA7TrcBDjc6RU-L68f5bba8v7mbz5aZ5ZTFTBhKXAG1Zs7JVE4ax2tmLCc1Nc6AoaowhpXGOgGiBgqgnDbOCpCG54pP0dlP7mrwbyOEWHVNsNC2ugc_hqqQucqppDyRp_-SiilZ5mWRwJM_4Ksfhz61qJTMmcjzFDdFxxtoNB3U1WpoOj2sq9-f8y_6rYUf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>756246651</pqid></control><display><type>article</type><title>Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Wu, Qiwei ; Chang, Weihua ; Rickers-Haunerland, Jutta ; Higo, Tobi ; Haunerland, Norbert H</creator><creatorcontrib>Wu, Qiwei ; Chang, Weihua ; Rickers-Haunerland, Jutta ; Higo, Tobi ; Haunerland, Norbert H</creatorcontrib><description>In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequences analyzed for potential regulatory elements. Comparison with other muscle FABP promoters revealed the presence of a 19-bp imperfect inverted repeat sequence that contains two hexanucleotide half sites (AGTGGT and ATGGGA), interspersed by 3 nucleotides. The promoter activity was studied with reporter gene constructs in L6 myoblasts, in which H-FABP expression is stimulated by long-chain fatty acids in a similar manner as in adult cardiomyocytes. The 19 bp element, located 180 bp upstream of the transcription start site, was found to be essential for the fatty acid induction of gene expression, and gel shift analysis confirmed that this fatty acid response element is capable of binding nuclear proteins both from rat myoblasts and locust muscle in the presence of fatty acids. A similar, but reverse sequence that is present upstream of all mammalian H-FABP promoters may modulate the expression of the rat H-FABP gene.</description><identifier>ISSN: 0300-8177</identifier><identifier>EISSN: 1573-4919</identifier><identifier>DOI: 10.1023/A:1020554824176</identifier><identifier>PMID: 12479583</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Base Sequence ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Cells, Cultured ; Fatty Acid-Binding Protein 7 ; Fatty Acid-Binding Proteins ; Fatty acids ; Fatty Acids - metabolism ; Gene Expression Regulation ; Grasshoppers - genetics ; Humans ; Male ; Muscle, Skeletal - cytology ; Muscle, Skeletal - physiology ; Muscles ; Myoblasts - cytology ; Myoblasts - physiology ; Neoplasm Proteins ; Nerve Tissue Proteins ; Protein Binding ; Proteins ; Rats ; Rats, Sprague-Dawley ; Recombinant Fusion Proteins - metabolism ; Response Elements ; Sequence Alignment ; Tumor Suppressor Proteins</subject><ispartof>Molecular and cellular biochemistry, 2002-10, Vol.239 (1-2), p.173-180</ispartof><rights>Kluwer Academic Publishers 2002</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-4b10f8eda2ff52415bf3d2bc30d1bfbeb178bb29bcf4e4de1ee7fabfc4e5b3673</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12479583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Qiwei</creatorcontrib><creatorcontrib>Chang, Weihua</creatorcontrib><creatorcontrib>Rickers-Haunerland, Jutta</creatorcontrib><creatorcontrib>Higo, Tobi</creatorcontrib><creatorcontrib>Haunerland, Norbert H</creatorcontrib><title>Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene</title><title>Molecular and cellular biochemistry</title><addtitle>Mol Cell Biochem</addtitle><description>In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequences analyzed for potential regulatory elements. Comparison with other muscle FABP promoters revealed the presence of a 19-bp imperfect inverted repeat sequence that contains two hexanucleotide half sites (AGTGGT and ATGGGA), interspersed by 3 nucleotides. The promoter activity was studied with reporter gene constructs in L6 myoblasts, in which H-FABP expression is stimulated by long-chain fatty acids in a similar manner as in adult cardiomyocytes. The 19 bp element, located 180 bp upstream of the transcription start site, was found to be essential for the fatty acid induction of gene expression, and gel shift analysis confirmed that this fatty acid response element is capable of binding nuclear proteins both from rat myoblasts and locust muscle in the presence of fatty acids. A similar, but reverse sequence that is present upstream of all mammalian H-FABP promoters may modulate the expression of the rat H-FABP gene.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Cells, Cultured</subject><subject>Fatty Acid-Binding Protein 7</subject><subject>Fatty Acid-Binding Proteins</subject><subject>Fatty acids</subject><subject>Fatty Acids - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Grasshoppers - genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Muscle, Skeletal - cytology</subject><subject>Muscle, Skeletal - physiology</subject><subject>Muscles</subject><subject>Myoblasts - cytology</subject><subject>Myoblasts - physiology</subject><subject>Neoplasm Proteins</subject><subject>Nerve Tissue Proteins</subject><subject>Protein Binding</subject><subject>Proteins</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Response Elements</subject><subject>Sequence Alignment</subject><subject>Tumor Suppressor Proteins</subject><issn>0300-8177</issn><issn>1573-4919</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kM1LxDAQxYMouq6evUnwoKdqPpvW27q4KizoQc8lSSdaaZu1SdH1rzfievEgDDzmzY8HbxA6ouScEsYvZpdJiJSiYIKqfAtNqFQ8EyUtt9GEcEKygiq1h_ZDeCWEpqG7aI8yoUpZ8Al6m7_oQdsIQ_OpY-N77B3WuId37HSMa6xtU-MBwsr3ATC00EEfcXzREVvfx8G3IW3p8rFKVNgkfDutt2OIuBuDbQEvZlcP-Bl6OEA7TrcBDjc6RU-L68f5bba8v7mbz5aZ5ZTFTBhKXAG1Zs7JVE4ax2tmLCc1Nc6AoaowhpXGOgGiBgqgnDbOCpCG54pP0dlP7mrwbyOEWHVNsNC2ugc_hqqQucqppDyRp_-SiilZ5mWRwJM_4Ksfhz61qJTMmcjzFDdFxxtoNB3U1WpoOj2sq9-f8y_6rYUf</recordid><startdate>20021001</startdate><enddate>20021001</enddate><creator>Wu, Qiwei</creator><creator>Chang, Weihua</creator><creator>Rickers-Haunerland, Jutta</creator><creator>Higo, Tobi</creator><creator>Haunerland, Norbert H</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20021001</creationdate><title>Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene</title><author>Wu, Qiwei ; Chang, Weihua ; Rickers-Haunerland, Jutta ; Higo, Tobi ; Haunerland, Norbert H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-4b10f8eda2ff52415bf3d2bc30d1bfbeb178bb29bcf4e4de1ee7fabfc4e5b3673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Cells, Cultured</topic><topic>Fatty Acid-Binding Protein 7</topic><topic>Fatty Acid-Binding Proteins</topic><topic>Fatty acids</topic><topic>Fatty Acids - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Grasshoppers - genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Muscle, Skeletal - cytology</topic><topic>Muscle, Skeletal - physiology</topic><topic>Muscles</topic><topic>Myoblasts - cytology</topic><topic>Myoblasts - physiology</topic><topic>Neoplasm Proteins</topic><topic>Nerve Tissue Proteins</topic><topic>Protein Binding</topic><topic>Proteins</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Response Elements</topic><topic>Sequence Alignment</topic><topic>Tumor Suppressor Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Qiwei</creatorcontrib><creatorcontrib>Chang, Weihua</creatorcontrib><creatorcontrib>Rickers-Haunerland, Jutta</creatorcontrib><creatorcontrib>Higo, Tobi</creatorcontrib><creatorcontrib>Haunerland, Norbert H</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Qiwei</au><au>Chang, Weihua</au><au>Rickers-Haunerland, Jutta</au><au>Higo, Tobi</au><au>Haunerland, Norbert H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene</atitle><jtitle>Molecular and cellular biochemistry</jtitle><addtitle>Mol Cell Biochem</addtitle><date>2002-10-01</date><risdate>2002</risdate><volume>239</volume><issue>1-2</issue><spage>173</spage><epage>180</epage><pages>173-180</pages><issn>0300-8177</issn><eissn>1573-4919</eissn><abstract>In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequences analyzed for potential regulatory elements. Comparison with other muscle FABP promoters revealed the presence of a 19-bp imperfect inverted repeat sequence that contains two hexanucleotide half sites (AGTGGT and ATGGGA), interspersed by 3 nucleotides. The promoter activity was studied with reporter gene constructs in L6 myoblasts, in which H-FABP expression is stimulated by long-chain fatty acids in a similar manner as in adult cardiomyocytes. The 19 bp element, located 180 bp upstream of the transcription start site, was found to be essential for the fatty acid induction of gene expression, and gel shift analysis confirmed that this fatty acid response element is capable of binding nuclear proteins both from rat myoblasts and locust muscle in the presence of fatty acids. A similar, but reverse sequence that is present upstream of all mammalian H-FABP promoters may modulate the expression of the rat H-FABP gene.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>12479583</pmid><doi>10.1023/A:1020554824176</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-8177 |
| ispartof | Molecular and cellular biochemistry, 2002-10, Vol.239 (1-2), p.173-180 |
| issn | 0300-8177 1573-4919 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_856761513 |
| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Base Sequence Carrier Proteins - genetics Carrier Proteins - metabolism Cells, Cultured Fatty Acid-Binding Protein 7 Fatty Acid-Binding Proteins Fatty acids Fatty Acids - metabolism Gene Expression Regulation Grasshoppers - genetics Humans Male Muscle, Skeletal - cytology Muscle, Skeletal - physiology Muscles Myoblasts - cytology Myoblasts - physiology Neoplasm Proteins Nerve Tissue Proteins Protein Binding Proteins Rats Rats, Sprague-Dawley Recombinant Fusion Proteins - metabolism Response Elements Sequence Alignment Tumor Suppressor Proteins |
| title | Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T07%3A32%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20a%20new%20fatty%20acid%20response%20element%20that%20controls%20the%20expression%20of%20the%20locust%20muscle%20FABP%20gene&rft.jtitle=Molecular%20and%20cellular%20biochemistry&rft.au=Wu,%20Qiwei&rft.date=2002-10-01&rft.volume=239&rft.issue=1-2&rft.spage=173&rft.epage=180&rft.pages=173-180&rft.issn=0300-8177&rft.eissn=1573-4919&rft_id=info:doi/10.1023/A:1020554824176&rft_dat=%3Cproquest_pubme%3E72759698%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=756246651&rft_id=info:pmid/12479583&rfr_iscdi=true |