Microbeam PIXE analysis of Na, K, Mg, and Ca in severely damaged cardiac tissue
The aim of the study was to measure with microbeam PIXE elements such as Na, K, Mg, and Ca in cardiac tissue after various treatments in vivo, which affect the cardiomyocyte integrity. It was assumed that local deviations from normal electrolyte levels indicate the degree of cardiac damage. The firs...
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| Veröffentlicht in: | Biological trace element research 1999-12, Vol.71-72 (1), p.57-64 |
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| creator | Verhoef, B A de Goeij, J J van der Vusse, G J de Voigt, M J |
| description | The aim of the study was to measure with microbeam PIXE elements such as Na, K, Mg, and Ca in cardiac tissue after various treatments in vivo, which affect the cardiomyocyte integrity. It was assumed that local deviations from normal electrolyte levels indicate the degree of cardiac damage. The first step in this feasibility study was comparison of severely damaged cardiac tissues with controls. Severe cardiac damage was introduced by the so-called Ca paradox. Experiments were performed with isolated rat hearts, perfused retrogradely with an oxygenated crystalloid buffer. Results indicated that severe cardiac damage was accompanied with almost complete disappearance of the normal intracellular electrolyte composition as a result of the loss of membrane integrity. Identifications of smaller and more locally present ischemic damages on basis of altered electrolyte levels appeared to be feasible. However, the prerequisite was that the mobility of electrolytes be kept under control during tissue sampling and sample preparation, when physiological mechanisms stop to maintain gradients. |
| doi_str_mv | 10.1007/BF02784191 |
| format | Article |
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It was assumed that local deviations from normal electrolyte levels indicate the degree of cardiac damage. The first step in this feasibility study was comparison of severely damaged cardiac tissues with controls. Severe cardiac damage was introduced by the so-called Ca paradox. Experiments were performed with isolated rat hearts, perfused retrogradely with an oxygenated crystalloid buffer. Results indicated that severe cardiac damage was accompanied with almost complete disappearance of the normal intracellular electrolyte composition as a result of the loss of membrane integrity. Identifications of smaller and more locally present ischemic damages on basis of altered electrolyte levels appeared to be feasible. However, the prerequisite was that the mobility of electrolytes be kept under control during tissue sampling and sample preparation, when physiological mechanisms stop to maintain gradients.</description><identifier>ISSN: 0163-4984</identifier><identifier>EISSN: 1559-0720</identifier><identifier>DOI: 10.1007/BF02784191</identifier><identifier>PMID: 10676479</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Calcium ; Calcium - analysis ; Cardiology ; Cardiomyocytes ; Damage ; Electrolytes ; Feasibility studies ; Heart ; In Vitro Techniques ; Integrity ; Ischemia ; L-Lactate Dehydrogenase - metabolism ; Magnesium ; Magnesium - analysis ; Male ; Microbeams ; Myocardium - chemistry ; Myocardium - enzymology ; Myocardium - pathology ; Potassium - analysis ; Rats ; Rats, Inbred Lew ; Sample preparation ; Sodium - analysis ; Spectrometry, X-Ray Emission ; Tissue ; Tissues</subject><ispartof>Biological trace element research, 1999-12, Vol.71-72 (1), p.57-64</ispartof><rights>Humana Press Inc. 1999</rights><rights>Humana Press Inc. 1999.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-8a7e9bf6b12293716da1cad2dbc0b2debc5c05bfc1da3bd9506a3403e9304ac53</citedby><cites>FETCH-LOGICAL-c370t-8a7e9bf6b12293716da1cad2dbc0b2debc5c05bfc1da3bd9506a3403e9304ac53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10676479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verhoef, B A</creatorcontrib><creatorcontrib>de Goeij, J J</creatorcontrib><creatorcontrib>van der Vusse, G J</creatorcontrib><creatorcontrib>de Voigt, M J</creatorcontrib><title>Microbeam PIXE analysis of Na, K, Mg, and Ca in severely damaged cardiac tissue</title><title>Biological trace element research</title><addtitle>Biol Trace Elem Res</addtitle><description>The aim of the study was to measure with microbeam PIXE elements such as Na, K, Mg, and Ca in cardiac tissue after various treatments in vivo, which affect the cardiomyocyte integrity. It was assumed that local deviations from normal electrolyte levels indicate the degree of cardiac damage. The first step in this feasibility study was comparison of severely damaged cardiac tissues with controls. Severe cardiac damage was introduced by the so-called Ca paradox. Experiments were performed with isolated rat hearts, perfused retrogradely with an oxygenated crystalloid buffer. Results indicated that severe cardiac damage was accompanied with almost complete disappearance of the normal intracellular electrolyte composition as a result of the loss of membrane integrity. Identifications of smaller and more locally present ischemic damages on basis of altered electrolyte levels appeared to be feasible. However, the prerequisite was that the mobility of electrolytes be kept under control during tissue sampling and sample preparation, when physiological mechanisms stop to maintain gradients.</description><subject>Animals</subject><subject>Calcium</subject><subject>Calcium - analysis</subject><subject>Cardiology</subject><subject>Cardiomyocytes</subject><subject>Damage</subject><subject>Electrolytes</subject><subject>Feasibility studies</subject><subject>Heart</subject><subject>In Vitro Techniques</subject><subject>Integrity</subject><subject>Ischemia</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Magnesium</subject><subject>Magnesium - analysis</subject><subject>Male</subject><subject>Microbeams</subject><subject>Myocardium - chemistry</subject><subject>Myocardium - enzymology</subject><subject>Myocardium - pathology</subject><subject>Potassium - analysis</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Sample preparation</subject><subject>Sodium - 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analysis</topic><topic>Cardiology</topic><topic>Cardiomyocytes</topic><topic>Damage</topic><topic>Electrolytes</topic><topic>Feasibility studies</topic><topic>Heart</topic><topic>In Vitro Techniques</topic><topic>Integrity</topic><topic>Ischemia</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Magnesium</topic><topic>Magnesium - analysis</topic><topic>Male</topic><topic>Microbeams</topic><topic>Myocardium - chemistry</topic><topic>Myocardium - enzymology</topic><topic>Myocardium - pathology</topic><topic>Potassium - analysis</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Sample preparation</topic><topic>Sodium - analysis</topic><topic>Spectrometry, X-Ray Emission</topic><topic>Tissue</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verhoef, B A</creatorcontrib><creatorcontrib>de Goeij, J J</creatorcontrib><creatorcontrib>van der Vusse, G J</creatorcontrib><creatorcontrib>de Voigt, M J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Biological trace element research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verhoef, B A</au><au>de Goeij, J J</au><au>van der Vusse, G J</au><au>de Voigt, M J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microbeam PIXE analysis of Na, K, Mg, and Ca in severely damaged cardiac tissue</atitle><jtitle>Biological trace element research</jtitle><addtitle>Biol Trace Elem Res</addtitle><date>1999-12-01</date><risdate>1999</risdate><volume>71-72</volume><issue>1</issue><spage>57</spage><epage>64</epage><pages>57-64</pages><issn>0163-4984</issn><eissn>1559-0720</eissn><abstract>The aim of the study was to measure with microbeam PIXE elements such as Na, K, Mg, and Ca in cardiac tissue after various treatments in vivo, which affect the cardiomyocyte integrity. It was assumed that local deviations from normal electrolyte levels indicate the degree of cardiac damage. The first step in this feasibility study was comparison of severely damaged cardiac tissues with controls. Severe cardiac damage was introduced by the so-called Ca paradox. Experiments were performed with isolated rat hearts, perfused retrogradely with an oxygenated crystalloid buffer. Results indicated that severe cardiac damage was accompanied with almost complete disappearance of the normal intracellular electrolyte composition as a result of the loss of membrane integrity. Identifications of smaller and more locally present ischemic damages on basis of altered electrolyte levels appeared to be feasible. However, the prerequisite was that the mobility of electrolytes be kept under control during tissue sampling and sample preparation, when physiological mechanisms stop to maintain gradients.</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>10676479</pmid><doi>10.1007/BF02784191</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Calcium Calcium - analysis Cardiology Cardiomyocytes Damage Electrolytes Feasibility studies Heart In Vitro Techniques Integrity Ischemia L-Lactate Dehydrogenase - metabolism Magnesium Magnesium - analysis Male Microbeams Myocardium - chemistry Myocardium - enzymology Myocardium - pathology Potassium - analysis Rats Rats, Inbred Lew Sample preparation Sodium - analysis Spectrometry, X-Ray Emission Tissue Tissues |
| title | Microbeam PIXE analysis of Na, K, Mg, and Ca in severely damaged cardiac tissue |
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