Quantification of 20-hydroxyeicosatetraenoic acid by colorimetric competitive enzyme linked immunosorbent assay

Analysis of 20-hydroxyeicosatetraenoic acid (20-HETE), a potent vasoconstrictor produced by the cytochrome P450 pathway, presently requires high-performance liquid chromatography (HPLC) and gas chromatography/ mass spectrometry (GC/MS). To simplify 20-HETE analysis, competitive ELISAs were developed...

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Veröffentlicht in:	Journal of biosciences 2003-02, Vol.28 (1), p.109-113
Hauptverfasser:	Grates, Harry E, McGowen, Richard M, Gupta, Smiti V, Falck, John R, Brown, Thomas R, Callewaert, Denis M, Sasaki, Diane M
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Biological samples Chromatography Colorimetry Enzyme-Linked Immunosorbent Assay - economics Enzymes Gas chromatography Hydroxyeicosatetraenoic Acids - analysis Hydroxyeicosatetraenoic Acids - blood Hydroxyeicosatetraenoic Acids - metabolism Hydroxyeicosatetraenoic Acids - urine Lipids Liquid chromatography Mass spectrometry Reproducibility of Results Sensitivity and Specificity Sorbents Vasoconstrictor Agents - analysis
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