Functional comparison of recombinant acidic mammalian chitinase with enzyme from murine bronchoalveolar lavage

Acidic mammalian chitinase (AMCase) is an enzyme that selectively degrades the biopolymer chitin. Several chitinase enzymes are utilized by mammals to hydrolyze chitin encountered by inhalation and ingestion. AMCase is distinct from other mammalian chitinases as its activity is retained in strongly...

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Veröffentlicht in:	Protein expression and purification 2011, Vol.75 (1), p.55-62
Hauptverfasser:	Goedken, Eric R., O’Brien, Robert F., Xiang, Tao, Banach, David L., Marchie, Susan C., Barlow, Eve H., Hubbard, Stacy, Mankovich, John A., Jiang, Jianjun, Richardson, Paul L., Cuff, Carolyn A., Cherniack, Andrew D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alveoli AMCase Amino Acid Sequence Animals Bronchoalveolar lavage Bronchoalveolar Lavage Fluid Cell Line Cercopithecus aethiops Chitin Chitinase Chitinases - genetics Chitinases - isolation & purification Chitinases - metabolism Cloning, Molecular - methods COS Cells Gene Expression Humans IL-13 Mice Molecular Sequence Data Peptides, Cyclic - metabolism Rats Rats, Sprague-Dawley Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism
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Beschreibung
Zusammenfassung:	Acidic mammalian chitinase (AMCase) is an enzyme that selectively degrades the biopolymer chitin. Several chitinase enzymes are utilized by mammals to hydrolyze chitin encountered by inhalation and ingestion. AMCase is distinct from other mammalian chitinases as its activity is retained in strongly acidic conditions (pH
ISSN:	1046-5928 1096-0279
DOI:	10.1016/j.pep.2010.08.013