Ovarian stimulation, in vitro fertilization, and effects of culture conditions on baboon preimplantation embryo development
Objective To evaluate the effects of ovarian stimulation and intracytoplasmic sperm injection (ICSI)-induced fertilization and efficacy of various culture systems on in vitro development of baboon embryos. Design In vitro study, animal model. Setting Research laboratory. Animal(s) Baboons in laborat...
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| Veröffentlicht in: | Fertility and sterility 2011-03, Vol.95 (4), p.1217-1223 |
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| creator | Chang, Tien-cheng, Ph.D Eddy, Carlton A., Ph.D Ying, Ying, Ph.D Liu, Ya-guang, M.D Holden, Alan E., Ph.D Brzyski, Robert G., M.D., Ph.D Schenken, Robert S., M.D |
| description | Objective To evaluate the effects of ovarian stimulation and intracytoplasmic sperm injection (ICSI)-induced fertilization and efficacy of various culture systems on in vitro development of baboon embryos. Design In vitro study, animal model. Setting Research laboratory. Animal(s) Baboons in laboratory animal research facility. Intervention(s) Baboons received FSH (75 IU daily) for 7 to 8 days and FSH/LH (75/75 IU daily) for 3 days, followed by hCG (2,000 IU). Oocytes were retrieved laparoscopically 36 hours after hCG. Intracytoplasmic sperm injection was performed on metaphase II (MII) oocytes. Fertilized embryos were placed into different culture conditions and feeder cell coculture. Embryo development was observed through the most advanced stages, including blastocyst formation. Main Outcome Measure(s) Oocytes retrieved, fertilization rates, multicell embryo rates, and blastocyst rates. Result(s) Baboon oocytes (n = 1,924, from 49 cycles) were retrieved. Significant heterogeneity was seen in ovarian response to exogenous gonadotropins and subsequent oocyte maturation. The percentage of MII oocytes showed no significant difference among individual female baboons and stimulation cycles. Nearly two thirds of MII oocytes were successfully fertilized with ICSI. Blastocyst rates varied significantly among embryos in different treatments. Coculture with feeder cells in P1/Blast, Quinn's Advantage, and Sydney IVF media generated better blastocyst rates. Conclusion(s) We tested multiple media and feeder cell combinations to optimize culture conditions in baboon embryo culture and obtained a high blastocyst rate similar to those reported for rhesus monkey embryos cultured in vitro, but still lower than with assisted reproductive technologies in women. |
| doi_str_mv | 10.1016/j.fertnstert.2010.06.095 |
| format | Article |
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Design In vitro study, animal model. Setting Research laboratory. Animal(s) Baboons in laboratory animal research facility. Intervention(s) Baboons received FSH (75 IU daily) for 7 to 8 days and FSH/LH (75/75 IU daily) for 3 days, followed by hCG (2,000 IU). Oocytes were retrieved laparoscopically 36 hours after hCG. Intracytoplasmic sperm injection was performed on metaphase II (MII) oocytes. Fertilized embryos were placed into different culture conditions and feeder cell coculture. Embryo development was observed through the most advanced stages, including blastocyst formation. Main Outcome Measure(s) Oocytes retrieved, fertilization rates, multicell embryo rates, and blastocyst rates. Result(s) Baboon oocytes (n = 1,924, from 49 cycles) were retrieved. Significant heterogeneity was seen in ovarian response to exogenous gonadotropins and subsequent oocyte maturation. The percentage of MII oocytes showed no significant difference among individual female baboons and stimulation cycles. Nearly two thirds of MII oocytes were successfully fertilized with ICSI. Blastocyst rates varied significantly among embryos in different treatments. Coculture with feeder cells in P1/Blast, Quinn's Advantage, and Sydney IVF media generated better blastocyst rates. Conclusion(s) We tested multiple media and feeder cell combinations to optimize culture conditions in baboon embryo culture and obtained a high blastocyst rate similar to those reported for rhesus monkey embryos cultured in vitro, but still lower than with assisted reproductive technologies in women.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2010.06.095</identifier><identifier>PMID: 20701907</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>animal models ; animal ovaries ; animal research ; Animals ; assisted reproductive technology ; baboon ; Biological and medical sciences ; Birth control ; blastocyst ; Cell Culture Techniques - methods ; coculture ; Coculture Techniques ; embryo culture ; Embryo, Mammalian - cytology ; Embryo, Mammalian - embryology ; embryogenesis ; Embryonic Development - physiology ; Female ; Fertilization in Vitro - methods ; follicle-stimulating hormone ; Gynecology. Andrology. Obstetrics ; human chorionic gonadotropin ; ICSI ; in vitro studies ; Internal Medicine ; intracytoplasmic sperm injection ; IVF ; laboratory animals ; luteinizing hormone ; Macaca mulatta ; Male ; Medical sciences ; metaphase ; Nonhuman primate ; Obstetrics and Gynecology ; oocytes ; ovarian stimulation ; Ovulation Induction - methods ; Papio ; Pregnancy ; Rats ; Sterility. Assisted procreation ; women</subject><ispartof>Fertility and sterility, 2011-03, Vol.95 (4), p.1217-1223</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2011 American Society for Reproductive Medicine</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c515t-aea397e39c0623926a80169175f8a98b0cb9660e1ed5269263e548f9189d9e783</citedby><cites>FETCH-LOGICAL-c515t-aea397e39c0623926a80169175f8a98b0cb9660e1ed5269263e548f9189d9e783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0015028210010502$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23960692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20701907$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chang, Tien-cheng, Ph.D</creatorcontrib><creatorcontrib>Eddy, Carlton A., Ph.D</creatorcontrib><creatorcontrib>Ying, Ying, Ph.D</creatorcontrib><creatorcontrib>Liu, Ya-guang, M.D</creatorcontrib><creatorcontrib>Holden, Alan E., Ph.D</creatorcontrib><creatorcontrib>Brzyski, Robert G., M.D., Ph.D</creatorcontrib><creatorcontrib>Schenken, Robert S., M.D</creatorcontrib><title>Ovarian stimulation, in vitro fertilization, and effects of culture conditions on baboon preimplantation embryo development</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To evaluate the effects of ovarian stimulation and intracytoplasmic sperm injection (ICSI)-induced fertilization and efficacy of various culture systems on in vitro development of baboon embryos. Design In vitro study, animal model. Setting Research laboratory. Animal(s) Baboons in laboratory animal research facility. Intervention(s) Baboons received FSH (75 IU daily) for 7 to 8 days and FSH/LH (75/75 IU daily) for 3 days, followed by hCG (2,000 IU). Oocytes were retrieved laparoscopically 36 hours after hCG. Intracytoplasmic sperm injection was performed on metaphase II (MII) oocytes. Fertilized embryos were placed into different culture conditions and feeder cell coculture. Embryo development was observed through the most advanced stages, including blastocyst formation. Main Outcome Measure(s) Oocytes retrieved, fertilization rates, multicell embryo rates, and blastocyst rates. Result(s) Baboon oocytes (n = 1,924, from 49 cycles) were retrieved. Significant heterogeneity was seen in ovarian response to exogenous gonadotropins and subsequent oocyte maturation. The percentage of MII oocytes showed no significant difference among individual female baboons and stimulation cycles. Nearly two thirds of MII oocytes were successfully fertilized with ICSI. Blastocyst rates varied significantly among embryos in different treatments. Coculture with feeder cells in P1/Blast, Quinn's Advantage, and Sydney IVF media generated better blastocyst rates. Conclusion(s) We tested multiple media and feeder cell combinations to optimize culture conditions in baboon embryo culture and obtained a high blastocyst rate similar to those reported for rhesus monkey embryos cultured in vitro, but still lower than with assisted reproductive technologies in women.</description><subject>animal models</subject><subject>animal ovaries</subject><subject>animal research</subject><subject>Animals</subject><subject>assisted reproductive technology</subject><subject>baboon</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>blastocyst</subject><subject>Cell Culture Techniques - methods</subject><subject>coculture</subject><subject>Coculture Techniques</subject><subject>embryo culture</subject><subject>Embryo, Mammalian - cytology</subject><subject>Embryo, Mammalian - embryology</subject><subject>embryogenesis</subject><subject>Embryonic Development - physiology</subject><subject>Female</subject><subject>Fertilization in Vitro - methods</subject><subject>follicle-stimulating hormone</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>human chorionic gonadotropin</subject><subject>ICSI</subject><subject>in vitro studies</subject><subject>Internal Medicine</subject><subject>intracytoplasmic sperm injection</subject><subject>IVF</subject><subject>laboratory animals</subject><subject>luteinizing hormone</subject><subject>Macaca mulatta</subject><subject>Male</subject><subject>Medical sciences</subject><subject>metaphase</subject><subject>Nonhuman primate</subject><subject>Obstetrics and Gynecology</subject><subject>oocytes</subject><subject>ovarian stimulation</subject><subject>Ovulation Induction - methods</subject><subject>Papio</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Sterility. Assisted procreation</subject><subject>women</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEokvhL4AvCA5kGSdrx75UgoovqVIPpWfLcSbIS2IvdrLSwp9nwi5U4oC4eKzxMx-ed4qCcVhz4PL1dt1jmkKe6FxXQG6Qa9DiXrHiQshSSFHfL1YAXJRQqeqseJTzFgAkb6qHxVkFDXANzar4cb23ydvA8uTHebCTj-EV84Ht_ZQiW8r4wX8_-W3oGPY9uimz2DM3D9OckLkYOr8Q5A2stW0ks0vox91gw_QrmOHYpkNkHe5xiLsRw_S4eNDbIeOTkz0vbt-_-3z5sby6_vDp8s1V6QQXU2nR1rrBWjuQVa0raRXNQPNG9Mpq1YJrtZSAHDtRSXqvUWxUr7nSncZG1efFi2PeXYrfZsyTGX12OFBvGOdslBAalNpsiHz5T5LLRom6ArEkVUfUpZhzwt7skh9tOhgOZhHJbM2dSGYRyYA0JBKFPj1VmdsRuz-Bv1Uh4PkJsNnZoU82OJ_vuFpLoI8S9-zI9TYa-yURc3tDlQQJXzWi1kS8PRJI8917TCY7j8Fh5xOpaLro_6ffi7-SuMEHT519xQPmbZxTIP0MN7kyYG6WtVu2jtMF6Fb_BC6n1Pg</recordid><startdate>20110315</startdate><enddate>20110315</enddate><creator>Chang, Tien-cheng, Ph.D</creator><creator>Eddy, Carlton A., Ph.D</creator><creator>Ying, Ying, Ph.D</creator><creator>Liu, Ya-guang, M.D</creator><creator>Holden, Alan E., Ph.D</creator><creator>Brzyski, Robert G., M.D., Ph.D</creator><creator>Schenken, Robert S., M.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope></search><sort><creationdate>20110315</creationdate><title>Ovarian stimulation, in vitro fertilization, and effects of culture conditions on baboon preimplantation embryo development</title><author>Chang, Tien-cheng, Ph.D ; Eddy, Carlton A., Ph.D ; Ying, Ying, Ph.D ; Liu, Ya-guang, M.D ; Holden, Alan E., Ph.D ; Brzyski, Robert G., M.D., Ph.D ; Schenken, Robert S., M.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c515t-aea397e39c0623926a80169175f8a98b0cb9660e1ed5269263e548f9189d9e783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>animal models</topic><topic>animal ovaries</topic><topic>animal research</topic><topic>Animals</topic><topic>assisted reproductive technology</topic><topic>baboon</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>blastocyst</topic><topic>Cell Culture Techniques - methods</topic><topic>coculture</topic><topic>Coculture Techniques</topic><topic>embryo culture</topic><topic>Embryo, Mammalian - cytology</topic><topic>Embryo, Mammalian - embryology</topic><topic>embryogenesis</topic><topic>Embryonic Development - physiology</topic><topic>Female</topic><topic>Fertilization in Vitro - methods</topic><topic>follicle-stimulating hormone</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>human chorionic gonadotropin</topic><topic>ICSI</topic><topic>in vitro studies</topic><topic>Internal Medicine</topic><topic>intracytoplasmic sperm injection</topic><topic>IVF</topic><topic>laboratory animals</topic><topic>luteinizing hormone</topic><topic>Macaca mulatta</topic><topic>Male</topic><topic>Medical sciences</topic><topic>metaphase</topic><topic>Nonhuman primate</topic><topic>Obstetrics and Gynecology</topic><topic>oocytes</topic><topic>ovarian stimulation</topic><topic>Ovulation Induction - methods</topic><topic>Papio</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Sterility. Assisted procreation</topic><topic>women</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chang, Tien-cheng, Ph.D</creatorcontrib><creatorcontrib>Eddy, Carlton A., Ph.D</creatorcontrib><creatorcontrib>Ying, Ying, Ph.D</creatorcontrib><creatorcontrib>Liu, Ya-guang, M.D</creatorcontrib><creatorcontrib>Holden, Alan E., Ph.D</creatorcontrib><creatorcontrib>Brzyski, Robert G., M.D., Ph.D</creatorcontrib><creatorcontrib>Schenken, Robert S., M.D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chang, Tien-cheng, Ph.D</au><au>Eddy, Carlton A., Ph.D</au><au>Ying, Ying, Ph.D</au><au>Liu, Ya-guang, M.D</au><au>Holden, Alan E., Ph.D</au><au>Brzyski, Robert G., M.D., Ph.D</au><au>Schenken, Robert S., M.D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ovarian stimulation, in vitro fertilization, and effects of culture conditions on baboon preimplantation embryo development</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2011-03-15</date><risdate>2011</risdate><volume>95</volume><issue>4</issue><spage>1217</spage><epage>1223</epage><pages>1217-1223</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective To evaluate the effects of ovarian stimulation and intracytoplasmic sperm injection (ICSI)-induced fertilization and efficacy of various culture systems on in vitro development of baboon embryos. Design In vitro study, animal model. Setting Research laboratory. Animal(s) Baboons in laboratory animal research facility. Intervention(s) Baboons received FSH (75 IU daily) for 7 to 8 days and FSH/LH (75/75 IU daily) for 3 days, followed by hCG (2,000 IU). Oocytes were retrieved laparoscopically 36 hours after hCG. Intracytoplasmic sperm injection was performed on metaphase II (MII) oocytes. Fertilized embryos were placed into different culture conditions and feeder cell coculture. Embryo development was observed through the most advanced stages, including blastocyst formation. Main Outcome Measure(s) Oocytes retrieved, fertilization rates, multicell embryo rates, and blastocyst rates. Result(s) Baboon oocytes (n = 1,924, from 49 cycles) were retrieved. Significant heterogeneity was seen in ovarian response to exogenous gonadotropins and subsequent oocyte maturation. The percentage of MII oocytes showed no significant difference among individual female baboons and stimulation cycles. Nearly two thirds of MII oocytes were successfully fertilized with ICSI. Blastocyst rates varied significantly among embryos in different treatments. Coculture with feeder cells in P1/Blast, Quinn's Advantage, and Sydney IVF media generated better blastocyst rates. Conclusion(s) We tested multiple media and feeder cell combinations to optimize culture conditions in baboon embryo culture and obtained a high blastocyst rate similar to those reported for rhesus monkey embryos cultured in vitro, but still lower than with assisted reproductive technologies in women.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>20701907</pmid><doi>10.1016/j.fertnstert.2010.06.095</doi><tpages>7</tpages></addata></record> |
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| ispartof | Fertility and sterility, 2011-03, Vol.95 (4), p.1217-1223 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | animal models animal ovaries animal research Animals assisted reproductive technology baboon Biological and medical sciences Birth control blastocyst Cell Culture Techniques - methods coculture Coculture Techniques embryo culture Embryo, Mammalian - cytology Embryo, Mammalian - embryology embryogenesis Embryonic Development - physiology Female Fertilization in Vitro - methods follicle-stimulating hormone Gynecology. Andrology. Obstetrics human chorionic gonadotropin ICSI in vitro studies Internal Medicine intracytoplasmic sperm injection IVF laboratory animals luteinizing hormone Macaca mulatta Male Medical sciences metaphase Nonhuman primate Obstetrics and Gynecology oocytes ovarian stimulation Ovulation Induction - methods Papio Pregnancy Rats Sterility. Assisted procreation women |
| title | Ovarian stimulation, in vitro fertilization, and effects of culture conditions on baboon preimplantation embryo development |
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