Construction of lentiviral vector containing SOX9 gene and its expression in bone marrow mesenchymal stem cells

To construct the lentiviral vector containing SOX9 gene and to detect its expression in MSCs derived from rabbit bone marrow. Human sox-9 gene coding region fragment was obtained by RT-PCR (reverse transcription-polymerase chain reaction) and then cloned into the plasmid of Pwpxl-MOD2 to form Pwpxl-...

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Veröffentlicht in:	Zhong hua yi xue za zhi 2010-12, Vol.90 (47), p.3376-3380
Hauptverfasser:	Xu, Yun, Chen, Liang, Zhu, Xue-Song, Shi, Qin, Liu, Yong, Gu, Yong, Tang, Tian-Si
Format:	Artikel
Sprache:	chi
Schlagworte:	Animals Bone Marrow Cells - metabolism Gene Expression Genetic Vectors Humans Lentivirus - genetics Mesenchymal Stromal Cells - metabolism Plasmids Rabbits SOX9 Transcription Factor - genetics Transfection
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