Purification and characterization of glutaminase-free l-asparaginase from Pectobacterium carotovorum MTCC 1428

An intracellular glutaminase-free l-asparaginase from Pectobacterium carotovorum MTCC 1428 was isolated to apparent homogeneity. The homotetramer enzyme has a molecular mass of 144.4 kDa (MALDI-TOF MS) and an isoelectric point of approximately 8.4. The enzyme is very specific for its natural substra...

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Veröffentlicht in:	Bioresource technology 2011, Vol.102 (2), p.2077-2082
Hauptverfasser:	Kumar, Sanjay, Venkata Dasu, V., Pakshirajan, K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Anti-leukemic enzyme Asparaginase - chemistry Asparaginase - isolation & purification Asparaginase - metabolism Biological and medical sciences Effectors Enzyme Stability Enzymes Fundamental and applied biological sciences. Psychology Glutaminase Glutaminase - metabolism Homogeneity Hydrogen-Ion Concentration Hydrolysis Kinetics l-Asparaginase Molecular Sequence Data Molecular Weight N-terminal sequence Optimization Pectobacterium Pectobacterium carotovorum Pectobacterium carotovorum - enzymology Phytopathology. Animal pests. Plant and forest protection Purification Sequence Alignment Sequence Analysis, Protein Side effects Substrate Specificity Temperature Uranium
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