Analysis of risperidone and 9-hydroxyrisperidone in human plasma, urine and saliva by MEPS-LC-UV
Risperidone is currently one of the most frequently prescribed atypical antipsychotic drugs; its main active metabolite 9-hydroxyrisperidone contributes significantly to the therapeutic effects observed. An original analytical method is presented for the simultaneous analysis of risperidone and the...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2011-01, Vol.879 (2), p.167-173 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Mandrioli, Roberto Mercolini, Laura Lateana, Domenico Boncompagni, Giancarlo Raggi, Maria Augusta |
| description | Risperidone is currently one of the most frequently prescribed atypical antipsychotic drugs; its main active metabolite 9-hydroxyrisperidone contributes significantly to the therapeutic effects observed. An original analytical method is presented for the simultaneous analysis of risperidone and the metabolite in plasma, urine and saliva by high-performance liquid chromatography coupled to an original sample pre-treatment procedure based on micro-extraction by packed sorbent (MEPS). The assays were carried out using a C8 reversed-phase column and a mobile phase composed of 73% (v/v) acidic phosphate buffer (30mM, pH 3.0) containing 0.23% triethylamine and 27% (v/v) acetonitrile. The UV detector was set at 238nm and diphenhydramine was used as the internal standard. The sample pre-treatment by MEPS was carried out on a C8 sorbent. The extraction yields values were higher than 92% for risperidone and 90% for 9-hydroxyrisperidone, with RSD for precision always lower than 7.9% for both analytes. Limit of quantification values in the different matrices were 4ng/mL or lower for risperidone and 6ng/mL or lower for the metabolite. The method was successfully applied to plasma, urine and saliva samples from psychotic patients undergoing therapy with risperidone, with satisfactory accuracy results (recovery>89%) and no interference from other drugs. Thus, the method seems to be suitable for the therapeutic drug monitoring of schizophrenic patients using the three different biological matrices plasma, urine and saliva. |
| doi_str_mv | 10.1016/j.jchromb.2010.11.033 |
| format | Article |
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An original analytical method is presented for the simultaneous analysis of risperidone and the metabolite in plasma, urine and saliva by high-performance liquid chromatography coupled to an original sample pre-treatment procedure based on micro-extraction by packed sorbent (MEPS). The assays were carried out using a C8 reversed-phase column and a mobile phase composed of 73% (v/v) acidic phosphate buffer (30mM, pH 3.0) containing 0.23% triethylamine and 27% (v/v) acetonitrile. The UV detector was set at 238nm and diphenhydramine was used as the internal standard. The sample pre-treatment by MEPS was carried out on a C8 sorbent. The extraction yields values were higher than 92% for risperidone and 90% for 9-hydroxyrisperidone, with RSD for precision always lower than 7.9% for both analytes. Limit of quantification values in the different matrices were 4ng/mL or lower for risperidone and 6ng/mL or lower for the metabolite. The method was successfully applied to plasma, urine and saliva samples from psychotic patients undergoing therapy with risperidone, with satisfactory accuracy results (recovery>89%) and no interference from other drugs. Thus, the method seems to be suitable for the therapeutic drug monitoring of schizophrenic patients using the three different biological matrices plasma, urine and saliva.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2010.11.033</identifier><identifier>PMID: 21183412</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>9-Hydroxyrisperidone ; acetonitrile ; Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; blood plasma ; Chromatography, Liquid - methods ; Drugs ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; high performance liquid chromatography ; Humans ; Isoxazoles - analysis ; Isoxazoles - blood ; Isoxazoles - chemistry ; Isoxazoles - urine ; Linear Models ; Mathematical analysis ; Matrices ; Matrix methods ; Medical sciences ; Metabolites ; Micro-extraction by packed sorbent (MEPS) ; microextraction ; monitoring ; Paliperidone Palmitate ; Patients ; Pharmacology. Drug treatments ; Plasma ; pretreatment ; Psychotic Disorders ; Pyrimidines - analysis ; Pyrimidines - blood ; Pyrimidines - chemistry ; Pyrimidines - urine ; Reproducibility of Results ; Risperidone ; Risperidone - analysis ; Risperidone - blood ; Risperidone - chemistry ; Risperidone - urine ; Saliva ; Saliva - chemistry ; Sensitivity and Specificity ; Solid Phase Microextraction - methods ; Sorbents ; Spectrophotometry, Ultraviolet ; therapeutics ; tranquilizers ; triethylamine ; Urine</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-01, Vol.879 (2), p.167-173</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-ef3224e88ac9e05e719ab450dbbfe74f0e3db616bb607bcd1edf5387a69579483</citedby><cites>FETCH-LOGICAL-c479t-ef3224e88ac9e05e719ab450dbbfe74f0e3db616bb607bcd1edf5387a69579483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1570023210007361$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23750828$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21183412$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mandrioli, Roberto</creatorcontrib><creatorcontrib>Mercolini, Laura</creatorcontrib><creatorcontrib>Lateana, Domenico</creatorcontrib><creatorcontrib>Boncompagni, Giancarlo</creatorcontrib><creatorcontrib>Raggi, Maria Augusta</creatorcontrib><title>Analysis of risperidone and 9-hydroxyrisperidone in human plasma, urine and saliva by MEPS-LC-UV</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>Risperidone is currently one of the most frequently prescribed atypical antipsychotic drugs; its main active metabolite 9-hydroxyrisperidone contributes significantly to the therapeutic effects observed. An original analytical method is presented for the simultaneous analysis of risperidone and the metabolite in plasma, urine and saliva by high-performance liquid chromatography coupled to an original sample pre-treatment procedure based on micro-extraction by packed sorbent (MEPS). The assays were carried out using a C8 reversed-phase column and a mobile phase composed of 73% (v/v) acidic phosphate buffer (30mM, pH 3.0) containing 0.23% triethylamine and 27% (v/v) acetonitrile. The UV detector was set at 238nm and diphenhydramine was used as the internal standard. The sample pre-treatment by MEPS was carried out on a C8 sorbent. The extraction yields values were higher than 92% for risperidone and 90% for 9-hydroxyrisperidone, with RSD for precision always lower than 7.9% for both analytes. Limit of quantification values in the different matrices were 4ng/mL or lower for risperidone and 6ng/mL or lower for the metabolite. The method was successfully applied to plasma, urine and saliva samples from psychotic patients undergoing therapy with risperidone, with satisfactory accuracy results (recovery>89%) and no interference from other drugs. Thus, the method seems to be suitable for the therapeutic drug monitoring of schizophrenic patients using the three different biological matrices plasma, urine and saliva.</description><subject>9-Hydroxyrisperidone</subject><subject>acetonitrile</subject><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>blood plasma</subject><subject>Chromatography, Liquid - methods</subject><subject>Drugs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>high performance liquid chromatography</subject><subject>Humans</subject><subject>Isoxazoles - analysis</subject><subject>Isoxazoles - blood</subject><subject>Isoxazoles - chemistry</subject><subject>Isoxazoles - urine</subject><subject>Linear Models</subject><subject>Mathematical analysis</subject><subject>Matrices</subject><subject>Matrix methods</subject><subject>Medical sciences</subject><subject>Metabolites</subject><subject>Micro-extraction by packed sorbent (MEPS)</subject><subject>microextraction</subject><subject>monitoring</subject><subject>Paliperidone Palmitate</subject><subject>Patients</subject><subject>Pharmacology. Drug treatments</subject><subject>Plasma</subject><subject>pretreatment</subject><subject>Psychotic Disorders</subject><subject>Pyrimidines - analysis</subject><subject>Pyrimidines - blood</subject><subject>Pyrimidines - chemistry</subject><subject>Pyrimidines - urine</subject><subject>Reproducibility of Results</subject><subject>Risperidone</subject><subject>Risperidone - analysis</subject><subject>Risperidone - blood</subject><subject>Risperidone - chemistry</subject><subject>Risperidone - urine</subject><subject>Saliva</subject><subject>Saliva - chemistry</subject><subject>Sensitivity and Specificity</subject><subject>Solid Phase Microextraction - methods</subject><subject>Sorbents</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>therapeutics</subject><subject>tranquilizers</subject><subject>triethylamine</subject><subject>Urine</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP0zAURiMEYh7wE4BsEBtSru04TlZoVM0AUhFIQxE748cNdZXExW5G5N_jqOGxm5Ut-1zf6-9k2TMCKwKkerNf7c0u-F6vKMxnZAWMPcjOSS1YwUT17WHacwEFUEbPsosY9wBEgGCPszNKSM1KQs-z71eD6qboYu7bPLh4wOCsHzBXg82bYjfZ4H9N_1-4Id-NvRryQ6dir17nY3ALH1Xn7lSup_zj9efbYrMutl-fZI9a1UV8uqyX2fbm-sv6fbH59O7D-mpTmFI0xwJbRmmJda1Mg8BRkEbpkoPVukVRtoDM6opUWlcgtLEEbctZLVTVcNGUNbvMXp3ePQT_c8R4lL2LBrtODejHKGuewhBQw_1kOUfDKU0kP5Em-BgDtvIQXK_CJAnI2YLcy8WCnC1IQmSykOqeLx1G3aP9W_Un9gS8XAAVjeraoAbj4j-OCQ41nT_14sS1ykv1I2mQ29vUiQOASGnwRLw9EZiyvXMYZDQOB4PWBTRHab27Z9jf-QOwxQ</recordid><startdate>20110115</startdate><enddate>20110115</enddate><creator>Mandrioli, Roberto</creator><creator>Mercolini, Laura</creator><creator>Lateana, Domenico</creator><creator>Boncompagni, Giancarlo</creator><creator>Raggi, Maria Augusta</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20110115</creationdate><title>Analysis of risperidone and 9-hydroxyrisperidone in human plasma, urine and saliva by MEPS-LC-UV</title><author>Mandrioli, Roberto ; Mercolini, Laura ; Lateana, Domenico ; Boncompagni, Giancarlo ; Raggi, Maria Augusta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-ef3224e88ac9e05e719ab450dbbfe74f0e3db616bb607bcd1edf5387a69579483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>9-Hydroxyrisperidone</topic><topic>acetonitrile</topic><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>blood plasma</topic><topic>Chromatography, Liquid - methods</topic><topic>Drugs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>high performance liquid chromatography</topic><topic>Humans</topic><topic>Isoxazoles - analysis</topic><topic>Isoxazoles - blood</topic><topic>Isoxazoles - chemistry</topic><topic>Isoxazoles - urine</topic><topic>Linear Models</topic><topic>Mathematical analysis</topic><topic>Matrices</topic><topic>Matrix methods</topic><topic>Medical sciences</topic><topic>Metabolites</topic><topic>Micro-extraction by packed sorbent (MEPS)</topic><topic>microextraction</topic><topic>monitoring</topic><topic>Paliperidone Palmitate</topic><topic>Patients</topic><topic>Pharmacology. Drug treatments</topic><topic>Plasma</topic><topic>pretreatment</topic><topic>Psychotic Disorders</topic><topic>Pyrimidines - analysis</topic><topic>Pyrimidines - blood</topic><topic>Pyrimidines - chemistry</topic><topic>Pyrimidines - urine</topic><topic>Reproducibility of Results</topic><topic>Risperidone</topic><topic>Risperidone - analysis</topic><topic>Risperidone - blood</topic><topic>Risperidone - chemistry</topic><topic>Risperidone - urine</topic><topic>Saliva</topic><topic>Saliva - chemistry</topic><topic>Sensitivity and Specificity</topic><topic>Solid Phase Microextraction - methods</topic><topic>Sorbents</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>therapeutics</topic><topic>tranquilizers</topic><topic>triethylamine</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mandrioli, Roberto</creatorcontrib><creatorcontrib>Mercolini, Laura</creatorcontrib><creatorcontrib>Lateana, Domenico</creatorcontrib><creatorcontrib>Boncompagni, Giancarlo</creatorcontrib><creatorcontrib>Raggi, Maria Augusta</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mandrioli, Roberto</au><au>Mercolini, Laura</au><au>Lateana, Domenico</au><au>Boncompagni, Giancarlo</au><au>Raggi, Maria Augusta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of risperidone and 9-hydroxyrisperidone in human plasma, urine and saliva by MEPS-LC-UV</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2011-01-15</date><risdate>2011</risdate><volume>879</volume><issue>2</issue><spage>167</spage><epage>173</epage><pages>167-173</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>Risperidone is currently one of the most frequently prescribed atypical antipsychotic drugs; its main active metabolite 9-hydroxyrisperidone contributes significantly to the therapeutic effects observed. An original analytical method is presented for the simultaneous analysis of risperidone and the metabolite in plasma, urine and saliva by high-performance liquid chromatography coupled to an original sample pre-treatment procedure based on micro-extraction by packed sorbent (MEPS). The assays were carried out using a C8 reversed-phase column and a mobile phase composed of 73% (v/v) acidic phosphate buffer (30mM, pH 3.0) containing 0.23% triethylamine and 27% (v/v) acetonitrile. The UV detector was set at 238nm and diphenhydramine was used as the internal standard. The sample pre-treatment by MEPS was carried out on a C8 sorbent. The extraction yields values were higher than 92% for risperidone and 90% for 9-hydroxyrisperidone, with RSD for precision always lower than 7.9% for both analytes. Limit of quantification values in the different matrices were 4ng/mL or lower for risperidone and 6ng/mL or lower for the metabolite. The method was successfully applied to plasma, urine and saliva samples from psychotic patients undergoing therapy with risperidone, with satisfactory accuracy results (recovery>89%) and no interference from other drugs. Thus, the method seems to be suitable for the therapeutic drug monitoring of schizophrenic patients using the three different biological matrices plasma, urine and saliva.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>21183412</pmid><doi>10.1016/j.jchromb.2010.11.033</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2011-01, Vol.879 (2), p.167-173 |
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| subjects | 9-Hydroxyrisperidone acetonitrile Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences blood plasma Chromatography, Liquid - methods Drugs Fundamental and applied biological sciences. Psychology General pharmacology high performance liquid chromatography Humans Isoxazoles - analysis Isoxazoles - blood Isoxazoles - chemistry Isoxazoles - urine Linear Models Mathematical analysis Matrices Matrix methods Medical sciences Metabolites Micro-extraction by packed sorbent (MEPS) microextraction monitoring Paliperidone Palmitate Patients Pharmacology. Drug treatments Plasma pretreatment Psychotic Disorders Pyrimidines - analysis Pyrimidines - blood Pyrimidines - chemistry Pyrimidines - urine Reproducibility of Results Risperidone Risperidone - analysis Risperidone - blood Risperidone - chemistry Risperidone - urine Saliva Saliva - chemistry Sensitivity and Specificity Solid Phase Microextraction - methods Sorbents Spectrophotometry, Ultraviolet therapeutics tranquilizers triethylamine Urine |
| title | Analysis of risperidone and 9-hydroxyrisperidone in human plasma, urine and saliva by MEPS-LC-UV |
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