A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change
TePixD, a cyanobacterial sensor of blue light using flavin adenine dinucleotide (FAD) (BLUF) which exists in a decamer form, was found to exhibit photoreaction sensitive to light intensity. While the number of excited molecules increased monotonically as the laser power increased, the number of deca...
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| Veröffentlicht in: | FEBS letters 2011-03, Vol.585 (5), p.786-790 |
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| container_title | FEBS letters |
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| creator | Tanaka, Keisuke Nakasone, Yusuke Okajima, Koji Ikeuchi, Masahiko Tokutomi, Satoru Terazima, Masahide |
| description | TePixD, a cyanobacterial sensor of blue light using flavin adenine dinucleotide (FAD) (BLUF) which exists in a decamer form, was found to exhibit photoreaction sensitive to light intensity. While the number of excited molecules increased monotonically as the laser power increased, the number of decamers exhibiting a global conformational change initially increased, and then decreased with the increase of excitation intensity. This unusual power dependence was analyzed based on a Poisson distribution equation, demonstrating that decamers containing more than one excited monomer subunit do not undergo conformational change. Our results suggest that TePixD functions not only as a photosensor, but also by sensing light intensity. |
| doi_str_mv | 10.1016/j.febslet.2011.02.003 |
| format | Article |
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Our results suggest that TePixD functions not only as a photosensor, but also by sensing light intensity.</description><subject>BLUF</subject><subject>Conformational change</subject><subject>Cyanobacteria - metabolism</subject><subject>Cyanobacteria - radiation effects</subject><subject>Diffusion</subject><subject>diffusion coefficient</subject><subject>diffusion-sensitive conformational change</subject><subject>DSCC</subject><subject>FAD</subject><subject>flavin adenine dinucleotide</subject><subject>Lasers</subject><subject>Light</subject><subject>Light Signal Transduction - radiation effects</subject><subject>Photoreaction</subject><subject>Photoreceptors, Microbial - chemistry</subject><subject>Photoreceptors, Microbial - metabolism</subject><subject>PixD</subject><subject>Protein Conformation</subject><subject>sensors of blue light using FAD</subject><subject>Sensors of blue light using flavin adenine dinucleotide</subject><subject>transient absorption</subject><subject>transient grating</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvhJ4B845QwtuO1wwW1pUuRFoFEe7a8yaTrlTcOsUO7_x6HXbjCaTTSe29G3yPkNYOSAVu-25UdbqLHVHJgrAReAognZMG0EoWolvopWQCwqpCqFmfkRYw7yLtm9XNyxpnIIZItyHRBH-yBpkAj9hGpd_fbRF2f8ubS4T39MvnkBo8FPjYu2eRCT0NH0xbp5fpuRYdtSGHEBoc86C1-c48faZyGYcQYMdIm9F0Y97-N1tNma_t7fEmeddZHfHWa5-RudX17dVOsv376fHWxLpqqlqyQWoDgQkKNXVUhAsqNXbINF9hyC1rqulJaqc4Cr1toFZN1p9EqXTEhNBPn5O0xdxjDjwljMnsXG_Te9himaLSUHLhSMivlUdmMIcYROzOMbm_Hg2FgZuBmZ07AzQzcADcZePa9OV2YNnts_7r-EM6Cm6PgwXk8_F-qWV1f8u9ze3N5jOXiapijPhyjMCP76XA0sXHYN9i6XEAybXD_-PYXYnip8g</recordid><startdate>20110309</startdate><enddate>20110309</enddate><creator>Tanaka, Keisuke</creator><creator>Nakasone, Yusuke</creator><creator>Okajima, Koji</creator><creator>Ikeuchi, Masahiko</creator><creator>Tokutomi, Satoru</creator><creator>Terazima, Masahide</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110309</creationdate><title>A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change</title><author>Tanaka, Keisuke ; 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| ispartof | FEBS letters, 2011-03, Vol.585 (5), p.786-790 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | BLUF Conformational change Cyanobacteria - metabolism Cyanobacteria - radiation effects Diffusion diffusion coefficient diffusion-sensitive conformational change DSCC FAD flavin adenine dinucleotide Lasers Light Light Signal Transduction - radiation effects Photoreaction Photoreceptors, Microbial - chemistry Photoreceptors, Microbial - metabolism PixD Protein Conformation sensors of blue light using FAD Sensors of blue light using flavin adenine dinucleotide transient absorption transient grating |
| title | A way to sense light intensity: Multiple-excitation of the BLUF photoreceptor TePixD suppresses conformational change |
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