Disposable flow cytometer with high efficiency in particle counting and sizing using an optofluidic lens
Flow cytometers are widely applied to environmental monitoring, industrial testing, and biochemical studies. Integrating a flow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further impr...
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| Veröffentlicht in: | Optics letters 2011-03, Vol.36 (5), p.657-659 |
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| creator | CHAOLONG SONG LUONG, Trung-Dung TIAN FOOK KONG NGUYEN, Nam-Trung ANAND KRISHNA ASUNDI |
| description | Flow cytometers are widely applied to environmental monitoring, industrial testing, and biochemical studies. Integrating a flow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this Letter, we propose a flow cytometer using an optofluidic lens to focus the light beam. Benefiting from the smooth liquid-liquid lens interface and the refractive-index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced, owing to the small beam width at the beam waist. The device presents an efficient and accurate performance on both the counting and sizing of particles. |
| doi_str_mv | 10.1364/OL.36.000657 |
| format | Article |
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Integrating a flow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this Letter, we propose a flow cytometer using an optofluidic lens to focus the light beam. Benefiting from the smooth liquid-liquid lens interface and the refractive-index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced, owing to the small beam width at the beam waist. The device presents an efficient and accurate performance on both the counting and sizing of particles.</description><subject>Disposable Equipment</subject><subject>Equipment Design</subject><subject>Exact sciences and technology</subject><subject>Flow Cytometry - instrumentation</subject><subject>Fundamental areas of phenomenology (including applications)</subject><subject>Lenses</subject><subject>Light</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Optics</subject><subject>Physics</subject><subject>Scattering, Radiation</subject><issn>0146-9592</issn><issn>1539-4794</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0D1PwzAQBmALgaAUNmbkBbGQYucSxx5R-ZQidYE5chy7MUrjEDuqyq8nVQpMdzo9dzq9CF1RsqDAkvtVvgC2IISwNDtCM5qCiJJMJMdoRmjCIpGK-Ayde_-5NxnAKTqLx00uQMxQ_Wh957wsG41N47ZY7YLb6KB7vLWhxrVd11gbY5XVrdph2-JO9sGq0Ss3tMG2ayzbCnv7vW8HPw2w64IzzWArq3CjW3-BToxsvL481Dn6eH56X75G-erlbfmQRwpSHiKqTMkNkTwVQhIYf4-B8thwXQkwHEwFYFQpmCqzSmlhBMuo4VWmBafAM5ij2-lu17uvQftQbKxXumlkq93gC56mMaEk5qO8m6Tqnfe9NkXX243sdwUlxT7aYpUXwIop2pFfHw4P5UZXf_g3yxHcHID0Sjaml62y_t8lhDEKAD_XIoJO</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>CHAOLONG SONG</creator><creator>LUONG, Trung-Dung</creator><creator>TIAN FOOK KONG</creator><creator>NGUYEN, Nam-Trung</creator><creator>ANAND KRISHNA ASUNDI</creator><general>Optical Society of America</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110301</creationdate><title>Disposable flow cytometer with high efficiency in particle counting and sizing using an optofluidic lens</title><author>CHAOLONG SONG ; LUONG, Trung-Dung ; TIAN FOOK KONG ; NGUYEN, Nam-Trung ; ANAND KRISHNA ASUNDI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-1cfb8f0a8599a0359223182f8ed93f83fd33fcb96cb7dce9f9671f8d7e9813873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Disposable Equipment</topic><topic>Equipment Design</topic><topic>Exact sciences and technology</topic><topic>Flow Cytometry - instrumentation</topic><topic>Fundamental areas of phenomenology (including applications)</topic><topic>Lenses</topic><topic>Light</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Optics</topic><topic>Physics</topic><topic>Scattering, Radiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CHAOLONG SONG</creatorcontrib><creatorcontrib>LUONG, Trung-Dung</creatorcontrib><creatorcontrib>TIAN FOOK KONG</creatorcontrib><creatorcontrib>NGUYEN, Nam-Trung</creatorcontrib><creatorcontrib>ANAND KRISHNA ASUNDI</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Optics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CHAOLONG SONG</au><au>LUONG, Trung-Dung</au><au>TIAN FOOK KONG</au><au>NGUYEN, Nam-Trung</au><au>ANAND KRISHNA ASUNDI</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Disposable flow cytometer with high efficiency in particle counting and sizing using an optofluidic lens</atitle><jtitle>Optics letters</jtitle><addtitle>Opt Lett</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>36</volume><issue>5</issue><spage>657</spage><epage>659</epage><pages>657-659</pages><issn>0146-9592</issn><eissn>1539-4794</eissn><coden>OPLEDP</coden><abstract>Flow cytometers are widely applied to environmental monitoring, industrial testing, and biochemical studies. Integrating a flow cytometer into microfluidic networks helps to miniaturize the system and make it portable for field use. The integration of optical components, such as lenses, further improves the compactness and thus has been intensively studied recently. However, the current designs suffer from severe light scattering due to the roughness of the solid-based lens interface. In this Letter, we propose a flow cytometer using an optofluidic lens to focus the light beam. Benefiting from the smooth liquid-liquid lens interface and the refractive-index matching liquid as cladding streams, a light beam can be well focused without scattering. The variations of the signal peak values are reduced, owing to the small beam width at the beam waist. 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| fulltext | fulltext |
| identifier | ISSN: 0146-9592 |
| ispartof | Optics letters, 2011-03, Vol.36 (5), p.657-659 |
| issn | 0146-9592 1539-4794 |
| language | eng |
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| source | MEDLINE; Optica Publishing Group Journals (Optical Society) |
| subjects | Disposable Equipment Equipment Design Exact sciences and technology Flow Cytometry - instrumentation Fundamental areas of phenomenology (including applications) Lenses Light Microfluidic Analytical Techniques - instrumentation Optics Physics Scattering, Radiation |
| title | Disposable flow cytometer with high efficiency in particle counting and sizing using an optofluidic lens |
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