Effects of culture medium and formulation on the larvicidal activity of the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) against Aedes aegypti
[Display omitted] ▶ Medium containing sunflower seed promoted the greatest production of zoospores. ▶ The LC50 value was 43.9μl of total culture/ml against Aedes aegyti larvae. ▶ The formulation of culture with 2% pectin increased the larval mortality. In this work, we examined the production of inf...
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| Veröffentlicht in: | Acta tropica 2011-02, Vol.117 (2), p.114-118 |
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| creator | Maldonado-Blanco, María Guadalupe Leal-López, Erika Yazmín Ochoa-Salazar, Ozmel Alejandro Elías-Santos, Myriam Galán-Wong, Luis Jesús Quiroz-Martínez, Humberto |
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▶ Medium containing sunflower seed promoted the greatest production of zoospores. ▶ The LC50 value was 43.9μl of total culture/ml against Aedes aegyti larvae. ▶ The formulation of culture with 2% pectin increased the larval mortality.
In this work, we examined the production of infective zoospores of Lagenidium giganteum in four culture media, and the larvicidal activity of the cultures was determined against Aedes aegypti larvae, as well as the effect of polymer encapsulation. Medium containing sunflower seed extract showed the greatest production of zoospores, 5.92×106 zoospores/ml after six days of fermentation at 25±2°C and 150rpm shaking. This culture tested against A. aegypti 1st stage larvae caused different mortality rates at 24, 48 and 72h posttreatment. The LC50 obtained was 43.9, 41.1 and 42.9μl of total culture/ml, at 24, 48 and 72h posttreatment respectively, while the culture grown in medium with soybean meal showed 3–5 times higher LC50 values. Finally, the total culture including mycelium, zoospores and presporangia formulated with 2.5% pectin showed significantly higher mortality rates, around 100% more than the unformulated culture, whose values were from 40 to 1% at 3, 6, 9, and 12d posttreatment in the bioassays carried out in the laboratory to determine residual activity. |
| doi_str_mv | 10.1016/j.actatropica.2010.10.010 |
| format | Article |
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▶ Medium containing sunflower seed promoted the greatest production of zoospores. ▶ The LC50 value was 43.9μl of total culture/ml against Aedes aegyti larvae. ▶ The formulation of culture with 2% pectin increased the larval mortality.
In this work, we examined the production of infective zoospores of Lagenidium giganteum in four culture media, and the larvicidal activity of the cultures was determined against Aedes aegypti larvae, as well as the effect of polymer encapsulation. Medium containing sunflower seed extract showed the greatest production of zoospores, 5.92×106 zoospores/ml after six days of fermentation at 25±2°C and 150rpm shaking. This culture tested against A. aegypti 1st stage larvae caused different mortality rates at 24, 48 and 72h posttreatment. The LC50 obtained was 43.9, 41.1 and 42.9μl of total culture/ml, at 24, 48 and 72h posttreatment respectively, while the culture grown in medium with soybean meal showed 3–5 times higher LC50 values. Finally, the total culture including mycelium, zoospores and presporangia formulated with 2.5% pectin showed significantly higher mortality rates, around 100% more than the unformulated culture, whose values were from 40 to 1% at 3, 6, 9, and 12d posttreatment in the bioassays carried out in the laboratory to determine residual activity.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2010.10.010</identifier><identifier>PMID: 21056028</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Aedes ; Aedes aegypti ; Analysis of Variance ; Animals ; bioassays ; Biological and medical sciences ; culture media ; Culture Media - pharmacology ; encapsulation ; Entomopathogenic fungi ; fermentation ; General aspects ; Glycine max ; Helianthus ; Lagenidiales ; Lagenidium - drug effects ; Lagenidium - growth & development ; Lagenidium giganteum ; Larva ; larvae ; lethal concentration 50 ; Lethal Dose 50 ; Medical sciences ; mortality ; Mosquito Control ; Mosquitoes ; mycelium ; Oomycetes ; Oomycetes - drug effects ; pathogens ; pectins ; Pest Control, Biological ; polymers ; Seeds ; soybean meal ; sunflower seed ; Zoospores</subject><ispartof>Acta tropica, 2011-02, Vol.117 (2), p.114-118</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-90dff99a0e2fa233149804e106acd4fa5fe7877b5e1079360818912c4d600de93</citedby><cites>FETCH-LOGICAL-c462t-90dff99a0e2fa233149804e106acd4fa5fe7877b5e1079360818912c4d600de93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actatropica.2010.10.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23779018$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21056028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maldonado-Blanco, María Guadalupe</creatorcontrib><creatorcontrib>Leal-López, Erika Yazmín</creatorcontrib><creatorcontrib>Ochoa-Salazar, Ozmel Alejandro</creatorcontrib><creatorcontrib>Elías-Santos, Myriam</creatorcontrib><creatorcontrib>Galán-Wong, Luis Jesús</creatorcontrib><creatorcontrib>Quiroz-Martínez, Humberto</creatorcontrib><title>Effects of culture medium and formulation on the larvicidal activity of the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) against Aedes aegypti</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>[Display omitted]
▶ Medium containing sunflower seed promoted the greatest production of zoospores. ▶ The LC50 value was 43.9μl of total culture/ml against Aedes aegyti larvae. ▶ The formulation of culture with 2% pectin increased the larval mortality.
In this work, we examined the production of infective zoospores of Lagenidium giganteum in four culture media, and the larvicidal activity of the cultures was determined against Aedes aegypti larvae, as well as the effect of polymer encapsulation. Medium containing sunflower seed extract showed the greatest production of zoospores, 5.92×106 zoospores/ml after six days of fermentation at 25±2°C and 150rpm shaking. This culture tested against A. aegypti 1st stage larvae caused different mortality rates at 24, 48 and 72h posttreatment. The LC50 obtained was 43.9, 41.1 and 42.9μl of total culture/ml, at 24, 48 and 72h posttreatment respectively, while the culture grown in medium with soybean meal showed 3–5 times higher LC50 values. Finally, the total culture including mycelium, zoospores and presporangia formulated with 2.5% pectin showed significantly higher mortality rates, around 100% more than the unformulated culture, whose values were from 40 to 1% at 3, 6, 9, and 12d posttreatment in the bioassays carried out in the laboratory to determine residual activity.</description><subject>Aedes</subject><subject>Aedes aegypti</subject><subject>Analysis of Variance</subject><subject>Animals</subject><subject>bioassays</subject><subject>Biological and medical sciences</subject><subject>culture media</subject><subject>Culture Media - pharmacology</subject><subject>encapsulation</subject><subject>Entomopathogenic fungi</subject><subject>fermentation</subject><subject>General aspects</subject><subject>Glycine max</subject><subject>Helianthus</subject><subject>Lagenidiales</subject><subject>Lagenidium - drug effects</subject><subject>Lagenidium - growth & development</subject><subject>Lagenidium giganteum</subject><subject>Larva</subject><subject>larvae</subject><subject>lethal concentration 50</subject><subject>Lethal Dose 50</subject><subject>Medical sciences</subject><subject>mortality</subject><subject>Mosquito Control</subject><subject>Mosquitoes</subject><subject>mycelium</subject><subject>Oomycetes</subject><subject>Oomycetes - drug effects</subject><subject>pathogens</subject><subject>pectins</subject><subject>Pest Control, Biological</subject><subject>polymers</subject><subject>Seeds</subject><subject>soybean meal</subject><subject>sunflower seed</subject><subject>Zoospores</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAQxy0EokvhK4A5IOCQxXYeTnqrVuUhrdQDVOJmTZ1x6lUSp7az0n4dPilOdykckSyPPfObh-ZPyFvO1pzx6tNuDTpC9G6yGtaCPfjXyTwhK17LPKtEWTwlK8YYzySrfp6RFyHs0k_IUjwnZ4KzsmKiXpFfV8agjoE6Q_Xcx9kjHbC180BhbKlxfph7iNaNNJ14h7QHv7fattDTNIXd23hYkpfQ4ML9bKOjE8Q71-FIt5Bu-1Cusx2MEdPrw7UbDhojhotHAHoMHyl0YMcQ6SW2GChgd5iifUmeGegDvjrZc3Lz-erH5mu2vf7ybXO5zXRRiZg1rDWmaYChMCDynBdNzQrkrALdFgZKg7KW8rZMLtnkFat53XChi7ZirMUmPyfvj3Un7-5nDFENNmjsexjRzUHVZV7Usih5Ipsjqb0LwaNRk7cD-IPiTC0KqZ36RyG1KLSEkkm5r09d5tu06MfMP5Ik4N0JgKChNx5GbcNfLpeyYXzh3hw5A05B5xNz8z11KpPqdZWXIhGbI4Fpa3uLXgVtcdRJXp80V62z_zHwb5owwTQ</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Maldonado-Blanco, María Guadalupe</creator><creator>Leal-López, Erika Yazmín</creator><creator>Ochoa-Salazar, Ozmel Alejandro</creator><creator>Elías-Santos, Myriam</creator><creator>Galán-Wong, Luis Jesús</creator><creator>Quiroz-Martínez, Humberto</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>H98</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20110201</creationdate><title>Effects of culture medium and formulation on the larvicidal activity of the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) against Aedes aegypti</title><author>Maldonado-Blanco, María Guadalupe ; Leal-López, Erika Yazmín ; Ochoa-Salazar, Ozmel Alejandro ; Elías-Santos, Myriam ; Galán-Wong, Luis Jesús ; Quiroz-Martínez, Humberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-90dff99a0e2fa233149804e106acd4fa5fe7877b5e1079360818912c4d600de93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aedes</topic><topic>Aedes aegypti</topic><topic>Analysis of Variance</topic><topic>Animals</topic><topic>bioassays</topic><topic>Biological and medical sciences</topic><topic>culture media</topic><topic>Culture Media - pharmacology</topic><topic>encapsulation</topic><topic>Entomopathogenic fungi</topic><topic>fermentation</topic><topic>General aspects</topic><topic>Glycine max</topic><topic>Helianthus</topic><topic>Lagenidiales</topic><topic>Lagenidium - drug effects</topic><topic>Lagenidium - growth & development</topic><topic>Lagenidium giganteum</topic><topic>Larva</topic><topic>larvae</topic><topic>lethal concentration 50</topic><topic>Lethal Dose 50</topic><topic>Medical sciences</topic><topic>mortality</topic><topic>Mosquito Control</topic><topic>Mosquitoes</topic><topic>mycelium</topic><topic>Oomycetes</topic><topic>Oomycetes - drug effects</topic><topic>pathogens</topic><topic>pectins</topic><topic>Pest Control, Biological</topic><topic>polymers</topic><topic>Seeds</topic><topic>soybean meal</topic><topic>sunflower seed</topic><topic>Zoospores</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maldonado-Blanco, María Guadalupe</creatorcontrib><creatorcontrib>Leal-López, Erika Yazmín</creatorcontrib><creatorcontrib>Ochoa-Salazar, Ozmel Alejandro</creatorcontrib><creatorcontrib>Elías-Santos, Myriam</creatorcontrib><creatorcontrib>Galán-Wong, Luis Jesús</creatorcontrib><creatorcontrib>Quiroz-Martínez, Humberto</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maldonado-Blanco, María Guadalupe</au><au>Leal-López, Erika Yazmín</au><au>Ochoa-Salazar, Ozmel Alejandro</au><au>Elías-Santos, Myriam</au><au>Galán-Wong, Luis Jesús</au><au>Quiroz-Martínez, Humberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of culture medium and formulation on the larvicidal activity of the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) against Aedes aegypti</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>117</volume><issue>2</issue><spage>114</spage><epage>118</epage><pages>114-118</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>[Display omitted]
▶ Medium containing sunflower seed promoted the greatest production of zoospores. ▶ The LC50 value was 43.9μl of total culture/ml against Aedes aegyti larvae. ▶ The formulation of culture with 2% pectin increased the larval mortality.
In this work, we examined the production of infective zoospores of Lagenidium giganteum in four culture media, and the larvicidal activity of the cultures was determined against Aedes aegypti larvae, as well as the effect of polymer encapsulation. Medium containing sunflower seed extract showed the greatest production of zoospores, 5.92×106 zoospores/ml after six days of fermentation at 25±2°C and 150rpm shaking. This culture tested against A. aegypti 1st stage larvae caused different mortality rates at 24, 48 and 72h posttreatment. The LC50 obtained was 43.9, 41.1 and 42.9μl of total culture/ml, at 24, 48 and 72h posttreatment respectively, while the culture grown in medium with soybean meal showed 3–5 times higher LC50 values. Finally, the total culture including mycelium, zoospores and presporangia formulated with 2.5% pectin showed significantly higher mortality rates, around 100% more than the unformulated culture, whose values were from 40 to 1% at 3, 6, 9, and 12d posttreatment in the bioassays carried out in the laboratory to determine residual activity.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>21056028</pmid><doi>10.1016/j.actatropica.2010.10.010</doi><tpages>5</tpages></addata></record> |
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| ispartof | Acta tropica, 2011-02, Vol.117 (2), p.114-118 |
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| subjects | Aedes Aedes aegypti Analysis of Variance Animals bioassays Biological and medical sciences culture media Culture Media - pharmacology encapsulation Entomopathogenic fungi fermentation General aspects Glycine max Helianthus Lagenidiales Lagenidium - drug effects Lagenidium - growth & development Lagenidium giganteum Larva larvae lethal concentration 50 Lethal Dose 50 Medical sciences mortality Mosquito Control Mosquitoes mycelium Oomycetes Oomycetes - drug effects pathogens pectins Pest Control, Biological polymers Seeds soybean meal sunflower seed Zoospores |
| title | Effects of culture medium and formulation on the larvicidal activity of the mosquito pathogen Lagenidium giganteum (Oomycetes: Lagenidiales) against Aedes aegypti |
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