A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments

Methodological impediments have long been the main problem in estimating the ecological role of marine benthic ciliates. Percoll density centrifugation is currently the most efficient technique for extracting ciliates from fine-grained sediments, while the high cost and low density of Percoll limit...

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Veröffentlicht in:	European journal of protistology 2010-11, Vol.46 (4), p.263-270
Hauptverfasser:	Xu, Kuidong, Du, Yongfen, Lei, Yanli, Dai, Renhai
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal, plant and microbial ecology Animals Benthic ciliates Biological and medical sciences Centrifugation, Density Gradient - methods Ciliophora - isolation & purification Extraction efficiency Fundamental and applied biological sciences. Psychology General aspects and techniques General aspects. Techniques Geologic Sediments - parasitology Identification Ludox-QPS method Methods and techniques (sampling, tagging, trapping, modelling...) Nematoda Nematoda - isolation & purification Parasitology - methods Protozoa Silicon Dioxide Silver Proteins - metabolism Staining and Labeling - methods
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