Transcriptional analysis of Penaeus stylirostris densovirus genes
Abstract Penaeus stylirostris densovirus ( Pst DNV) genome contains three open reading frames (ORFs), left, middle, and right, which encode a non-structural (NS) protein, an unknown protein, and a capsid protein (CP), respectively. Transcription mapping revealed that P2, P11 and P61 promoters transc...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2010-06, Vol.402 (1), p.112-120 |
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| creator | Dhar, Arun K Kaizer, Krista N Lakshman, Dilip K |
| description | Abstract Penaeus stylirostris densovirus ( Pst DNV) genome contains three open reading frames (ORFs), left, middle, and right, which encode a non-structural (NS) protein, an unknown protein, and a capsid protein (CP), respectively. Transcription mapping revealed that P2, P11 and P61 promoters transcribe the left, middle and right ORFs. NS transcript uses the D1/A1 donor/acceptor sites for splicing and has two alternate transcription termination sites (TTS) that were different from the previously predicted TTS. The transcription initiation site (TIS) and the TTS for the middle and the right ORFs conform to predicted sites. Pst DNV transcript quantification in infected shrimp revealed that the NS and CP transcripts were expressed at an equivalent level and significantly higher than the middle ORF transcript. In vitro assay showed that P2 had the highest promoter activity followed by P11 and P61. Transcription mapping data provided new insights into Pst DNV gene expression strategy. |
| doi_str_mv | 10.1016/j.virol.2010.03.006 |
| format | Article |
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Transcription mapping revealed that P2, P11 and P61 promoters transcribe the left, middle and right ORFs. NS transcript uses the D1/A1 donor/acceptor sites for splicing and has two alternate transcription termination sites (TTS) that were different from the previously predicted TTS. The transcription initiation site (TIS) and the TTS for the middle and the right ORFs conform to predicted sites. Pst DNV transcript quantification in infected shrimp revealed that the NS and CP transcripts were expressed at an equivalent level and significantly higher than the middle ORF transcript. In vitro assay showed that P2 had the highest promoter activity followed by P11 and P61. Transcription mapping data provided new insights into Pst DNV gene expression strategy.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2010.03.006</identifier><identifier>PMID: 20381108</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Base Sequence ; coat proteins ; Densovirus ; Densovirus - genetics ; Densovirus - growth & development ; gene expression ; Gene Expression Profiling ; genes ; IHHNV ; Infectious Disease ; Infectious hypodermal and hematopoietic Necrosis virus ; Litopenaeus stylirostris ; messenger RNA ; Molecular Sequence Data ; open reading frames ; Penaeidae - virology ; Penaeus stylirostris ; Penaeus stylirostris densovirus ; promoter regions ; Promoter Regions, Genetic ; PstDNV ; Sequence Alignment ; Shrimp ; transcription (genetics) ; Transcription Initiation Site ; transcription termination sites ; Transcription, Genetic ; viral diseases of animals and humans ; viral proteins</subject><ispartof>Virology (New York, N.Y.), 2010-06, Vol.402 (1), p.112-120</ispartof><rights>Elsevier Inc.</rights><rights>2010 Elsevier Inc.</rights><rights>Copyright (c) 2010 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c514t-caa1fd5caa98b7dbac329181e6bb9fcf7ad8227b387152c1d78bec6d4b6aa7563</citedby><cites>FETCH-LOGICAL-c514t-caa1fd5caa98b7dbac329181e6bb9fcf7ad8227b387152c1d78bec6d4b6aa7563</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virol.2010.03.006$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20381108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dhar, Arun K</creatorcontrib><creatorcontrib>Kaizer, Krista N</creatorcontrib><creatorcontrib>Lakshman, Dilip K</creatorcontrib><title>Transcriptional analysis of Penaeus stylirostris densovirus genes</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Abstract Penaeus stylirostris densovirus ( Pst DNV) genome contains three open reading frames (ORFs), left, middle, and right, which encode a non-structural (NS) protein, an unknown protein, and a capsid protein (CP), respectively. Transcription mapping revealed that P2, P11 and P61 promoters transcribe the left, middle and right ORFs. NS transcript uses the D1/A1 donor/acceptor sites for splicing and has two alternate transcription termination sites (TTS) that were different from the previously predicted TTS. The transcription initiation site (TIS) and the TTS for the middle and the right ORFs conform to predicted sites. Pst DNV transcript quantification in infected shrimp revealed that the NS and CP transcripts were expressed at an equivalent level and significantly higher than the middle ORF transcript. In vitro assay showed that P2 had the highest promoter activity followed by P11 and P61. Transcription mapping data provided new insights into Pst DNV gene expression strategy.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>coat proteins</subject><subject>Densovirus</subject><subject>Densovirus - genetics</subject><subject>Densovirus - growth & development</subject><subject>gene expression</subject><subject>Gene Expression Profiling</subject><subject>genes</subject><subject>IHHNV</subject><subject>Infectious Disease</subject><subject>Infectious hypodermal and hematopoietic Necrosis virus</subject><subject>Litopenaeus stylirostris</subject><subject>messenger RNA</subject><subject>Molecular Sequence Data</subject><subject>open reading frames</subject><subject>Penaeidae - virology</subject><subject>Penaeus stylirostris</subject><subject>Penaeus stylirostris densovirus</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>PstDNV</subject><subject>Sequence Alignment</subject><subject>Shrimp</subject><subject>transcription (genetics)</subject><subject>Transcription Initiation Site</subject><subject>transcription termination sites</subject><subject>Transcription, Genetic</subject><subject>viral diseases of animals and humans</subject><subject>viral proteins</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhi0EokvhFyDB3jhlmYkT2zmAVFV8SZVAanu2HGdSeckmiyeptP-eWbZw4NKLR371zocfj1KvETYIaN5vN_cpT8OmBFFAbwDME7VCaEwBusKnagVQlYVxZXmmXjBvQe7WwnN1VoJ2iOBW6uImh5FjTvs5TWMY1kGOAydeT_36B42BFl7zfBikFc9Z9I5GnqSz6Hc0Er9Uz_owML16iOfq9vOnm8uvxdX3L98uL66KWGM1FzEE7LtaQuNa27Uh6rJBh2Tatuljb0Mng9pWO4t1GbGzrqVouqo1Idja6HP17lR3n6dfC_Hsd4kjDUMYaVrYu1pX1lVN9ajTal2X1jQgTn1yRnkdZ-r9PqddyAeP4I-Q_db_geyPkD1oL5Al681D_aXdUfcv5y9VMbw9Gfow-XAn1PzttVTQgM4g2EYcH04OEmL3ibLnmGiM1KVMcfbdlB4Z4eN_-XFIY4ph-EkH4u20ZPlH9ui59OCvj5twXASUHcC6qfVvqrOuBA</recordid><startdate>20100620</startdate><enddate>20100620</enddate><creator>Dhar, Arun K</creator><creator>Kaizer, Krista N</creator><creator>Lakshman, Dilip K</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20100620</creationdate><title>Transcriptional analysis of Penaeus stylirostris densovirus genes</title><author>Dhar, Arun K ; Kaizer, Krista N ; Lakshman, Dilip K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c514t-caa1fd5caa98b7dbac329181e6bb9fcf7ad8227b387152c1d78bec6d4b6aa7563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>coat proteins</topic><topic>Densovirus</topic><topic>Densovirus - genetics</topic><topic>Densovirus - growth & development</topic><topic>gene expression</topic><topic>Gene Expression Profiling</topic><topic>genes</topic><topic>IHHNV</topic><topic>Infectious Disease</topic><topic>Infectious hypodermal and hematopoietic Necrosis virus</topic><topic>Litopenaeus stylirostris</topic><topic>messenger RNA</topic><topic>Molecular Sequence Data</topic><topic>open reading frames</topic><topic>Penaeidae - virology</topic><topic>Penaeus stylirostris</topic><topic>Penaeus stylirostris densovirus</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>PstDNV</topic><topic>Sequence Alignment</topic><topic>Shrimp</topic><topic>transcription (genetics)</topic><topic>Transcription Initiation Site</topic><topic>transcription termination sites</topic><topic>Transcription, Genetic</topic><topic>viral diseases of animals and humans</topic><topic>viral proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dhar, Arun K</creatorcontrib><creatorcontrib>Kaizer, Krista N</creatorcontrib><creatorcontrib>Lakshman, Dilip K</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dhar, Arun K</au><au>Kaizer, Krista N</au><au>Lakshman, Dilip K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional analysis of Penaeus stylirostris densovirus genes</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2010-06-20</date><risdate>2010</risdate><volume>402</volume><issue>1</issue><spage>112</spage><epage>120</epage><pages>112-120</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Abstract Penaeus stylirostris densovirus ( Pst DNV) genome contains three open reading frames (ORFs), left, middle, and right, which encode a non-structural (NS) protein, an unknown protein, and a capsid protein (CP), respectively. Transcription mapping revealed that P2, P11 and P61 promoters transcribe the left, middle and right ORFs. NS transcript uses the D1/A1 donor/acceptor sites for splicing and has two alternate transcription termination sites (TTS) that were different from the previously predicted TTS. The transcription initiation site (TIS) and the TTS for the middle and the right ORFs conform to predicted sites. Pst DNV transcript quantification in infected shrimp revealed that the NS and CP transcripts were expressed at an equivalent level and significantly higher than the middle ORF transcript. In vitro assay showed that P2 had the highest promoter activity followed by P11 and P61. Transcription mapping data provided new insights into Pst DNV gene expression strategy.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>20381108</pmid><doi>10.1016/j.virol.2010.03.006</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Base Sequence coat proteins Densovirus Densovirus - genetics Densovirus - growth & development gene expression Gene Expression Profiling genes IHHNV Infectious Disease Infectious hypodermal and hematopoietic Necrosis virus Litopenaeus stylirostris messenger RNA Molecular Sequence Data open reading frames Penaeidae - virology Penaeus stylirostris Penaeus stylirostris densovirus promoter regions Promoter Regions, Genetic PstDNV Sequence Alignment Shrimp transcription (genetics) Transcription Initiation Site transcription termination sites Transcription, Genetic viral diseases of animals and humans viral proteins |
| title | Transcriptional analysis of Penaeus stylirostris densovirus genes |
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