Schistosoma japonicum: Efficient and rapid purification of the tetraspanin extracellular loop 2, a potential protective antigen against schistosomiasis in mammalian
The extracellular loop 2 of a tetraspanin from Schistosoma japonicum (Sj-TSP-2) is homologous to Schistosoma mansoni TSP-2. In our initial study, Sj-TSP-2 is an identical antigen against schistosomiasis caused by S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sephar...
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| Veröffentlicht in: | Experimental parasitology 2010-12, Vol.126 (4), p.456-461 |
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| creator | Yuan, Chuang Fu, Yi-jie Li, Jie Yue, Yu-fei Cai, Lu-lu Xiao, Wen-jing Chen, Jian-ping Yang, Li |
| description | The extracellular loop 2 of a tetraspanin from
Schistosoma japonicum (Sj-TSP-2) is homologous to
Schistosoma mansoni TSP-2. In our initial study, Sj-TSP-2 is an identical antigen against schistosomiasis caused by
S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sepharose, Sj-TSP-2 was expressed and purified as a soluble fusion constructed with an N-terminal thioredoxin-His
6-EK protease site tag (Trx-TSP-2). In phosphate buffer (PB) with a low concentration of imidazole, the Trx-TSP-2 fusion protein was efficiently cleaved by enterokinase (EK). Sj-TSP-2 was isolated and enriched using cobalt (Co)-absorbed chelating Sepharose and HiTrap SP column. Character of the protein was analyzed via animal experiments and then clinical trials. The purification approach yielded pure Sj-TSP-2, which will provide feasible advices for discovering vaccines against schistosomiasis. |
| doi_str_mv | 10.1016/j.exppara.2010.05.018 |
| format | Article |
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Schistosoma japonicum (Sj-TSP-2) is homologous to
Schistosoma mansoni TSP-2. In our initial study, Sj-TSP-2 is an identical antigen against schistosomiasis caused by
S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sepharose, Sj-TSP-2 was expressed and purified as a soluble fusion constructed with an N-terminal thioredoxin-His
6-EK protease site tag (Trx-TSP-2). In phosphate buffer (PB) with a low concentration of imidazole, the Trx-TSP-2 fusion protein was efficiently cleaved by enterokinase (EK). Sj-TSP-2 was isolated and enriched using cobalt (Co)-absorbed chelating Sepharose and HiTrap SP column. Character of the protein was analyzed via animal experiments and then clinical trials. The purification approach yielded pure Sj-TSP-2, which will provide feasible advices for discovering vaccines against schistosomiasis.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2010.05.018</identifier><identifier>PMID: 20685202</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Adolescent ; Adult ; Amino Acid Sequence ; Animals ; Antibodies, Helminth - blood ; Antigens, Helminth - chemistry ; Antigens, Helminth - immunology ; Antigens, Helminth - isolation & purification ; Biological and medical sciences ; Child ; Co-affinity ; Enterokinase (Enzyme Commission Number: 232-761-1) ; Enteropeptidase - metabolism ; Female ; Fluke ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Humans ; Life cycle. Host-agent relationship. Pathogenesis ; Male ; Mammalia ; Membrane Proteins - chemistry ; Membrane Proteins - immunology ; Membrane Proteins - isolation & purification ; Mice ; Nerve Tissue Proteins - chemistry ; Nerve Tissue Proteins - immunology ; Nerve Tissue Proteins - isolation & purification ; pET32 ; Plasmids ; Protozoa ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - isolation & purification ; Recombinant Fusion Proteins - metabolism ; Schistosoma japonicum ; Schistosoma japonicum - immunology ; Schistosoma mansoni ; Schistosomiasis japonica - immunology ; Schistosomiasis japonica - prevention & control ; Sequence Alignment ; Small peptide ; Tetraspanin ; Tetraspanins ; Trx-Tag ; Vaccination ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution ; Young Adult</subject><ispartof>Experimental parasitology, 2010-12, Vol.126 (4), p.456-461</ispartof><rights>2010 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-f94279f9ab915a0854e5c107b463fdf8ccf6cd36f9c2654dd2e4226a1a2b96993</citedby><cites>FETCH-LOGICAL-c426t-f94279f9ab915a0854e5c107b463fdf8ccf6cd36f9c2654dd2e4226a1a2b96993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2010.05.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23252429$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20685202$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, Chuang</creatorcontrib><creatorcontrib>Fu, Yi-jie</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Yue, Yu-fei</creatorcontrib><creatorcontrib>Cai, Lu-lu</creatorcontrib><creatorcontrib>Xiao, Wen-jing</creatorcontrib><creatorcontrib>Chen, Jian-ping</creatorcontrib><creatorcontrib>Yang, Li</creatorcontrib><title>Schistosoma japonicum: Efficient and rapid purification of the tetraspanin extracellular loop 2, a potential protective antigen against schistosomiasis in mammalian</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>The extracellular loop 2 of a tetraspanin from
Schistosoma japonicum (Sj-TSP-2) is homologous to
Schistosoma mansoni TSP-2. In our initial study, Sj-TSP-2 is an identical antigen against schistosomiasis caused by
S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sepharose, Sj-TSP-2 was expressed and purified as a soluble fusion constructed with an N-terminal thioredoxin-His
6-EK protease site tag (Trx-TSP-2). In phosphate buffer (PB) with a low concentration of imidazole, the Trx-TSP-2 fusion protein was efficiently cleaved by enterokinase (EK). Sj-TSP-2 was isolated and enriched using cobalt (Co)-absorbed chelating Sepharose and HiTrap SP column. Character of the protein was analyzed via animal experiments and then clinical trials. The purification approach yielded pure Sj-TSP-2, which will provide feasible advices for discovering vaccines against schistosomiasis.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens, Helminth - chemistry</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Co-affinity</subject><subject>Enterokinase (Enzyme Commission Number: 232-761-1)</subject><subject>Enteropeptidase - metabolism</subject><subject>Female</subject><subject>Fluke</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Life cycle. Host-agent relationship. 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Psychology</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Life cycle. Host-agent relationship. 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Schistosoma japonicum (Sj-TSP-2) is homologous to
Schistosoma mansoni TSP-2. In our initial study, Sj-TSP-2 is an identical antigen against schistosomiasis caused by
S. japonicum. Through the pET32 vector system and nickel (Ni)-absorbed chelating Sepharose, Sj-TSP-2 was expressed and purified as a soluble fusion constructed with an N-terminal thioredoxin-His
6-EK protease site tag (Trx-TSP-2). In phosphate buffer (PB) with a low concentration of imidazole, the Trx-TSP-2 fusion protein was efficiently cleaved by enterokinase (EK). Sj-TSP-2 was isolated and enriched using cobalt (Co)-absorbed chelating Sepharose and HiTrap SP column. Character of the protein was analyzed via animal experiments and then clinical trials. The purification approach yielded pure Sj-TSP-2, which will provide feasible advices for discovering vaccines against schistosomiasis.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>20685202</pmid><doi>10.1016/j.exppara.2010.05.018</doi><tpages>6</tpages></addata></record> |
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| ispartof | Experimental parasitology, 2010-12, Vol.126 (4), p.456-461 |
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| subjects | Adolescent Adult Amino Acid Sequence Animals Antibodies, Helminth - blood Antigens, Helminth - chemistry Antigens, Helminth - immunology Antigens, Helminth - isolation & purification Biological and medical sciences Child Co-affinity Enterokinase (Enzyme Commission Number: 232-761-1) Enteropeptidase - metabolism Female Fluke Fundamental and applied biological sciences. Psychology Gene Expression Humans Life cycle. Host-agent relationship. Pathogenesis Male Mammalia Membrane Proteins - chemistry Membrane Proteins - immunology Membrane Proteins - isolation & purification Mice Nerve Tissue Proteins - chemistry Nerve Tissue Proteins - immunology Nerve Tissue Proteins - isolation & purification pET32 Plasmids Protozoa Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism Schistosoma japonicum Schistosoma japonicum - immunology Schistosoma mansoni Schistosomiasis japonica - immunology Schistosomiasis japonica - prevention & control Sequence Alignment Small peptide Tetraspanin Tetraspanins Trx-Tag Vaccination Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Young Adult |
| title | Schistosoma japonicum: Efficient and rapid purification of the tetraspanin extracellular loop 2, a potential protective antigen against schistosomiasis in mammalian |
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