Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe
Phytophthora nicotianae and Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-sp...
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| Veröffentlicht in: | Journal of microbiological methods 2010-11, Vol.83 (2), p.260-265 |
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| container_title | Journal of microbiological methods |
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| creator | Ahonsi, Monday O. Ling, Yin Kageyama, Koji |
| description | Phytophthora nicotianae and
Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR. |
| doi_str_mv | 10.1016/j.mimet.2010.08.019 |
| format | Article |
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Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR.</description><identifier>ISSN: 0167-7012</identifier><identifier>EISSN: 1872-8359</identifier><identifier>DOI: 10.1016/j.mimet.2010.08.019</identifier><identifier>PMID: 20826191</identifier><identifier>CODEN: JMIMDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; DNA, Fungal - chemistry ; DNA, Fungal - genetics ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Japan ; Kalanchoe ; Kalanchoe - microbiology ; Microbiology ; Molecular Sequence Data ; Multiplex PCR ; Mycological methods and techniques used in mycology ; Mycology ; Mycology - methods ; Oomycetes ; Phytopathology. Animal pests. Plant and forest protection ; Phytophthora - genetics ; Phytophthora - isolation & purification ; Phytophthora nicotianae ; Plant Diseases - microbiology ; Polymerase Chain Reaction - methods ; Pythium ; Pythium - genetics ; Pythium - isolation & purification ; Recycled irrigation ; SCAR markers ; Sequence Analysis, DNA ; Sustainable disease management strategies</subject><ispartof>Journal of microbiological methods, 2010-11, Vol.83 (2), p.260-265</ispartof><rights>2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-b76119696328cf5d7f066f2d4102987fad77bf8b24833fa94cc3dc4b33e0d7513</citedby><cites>FETCH-LOGICAL-c420t-b76119696328cf5d7f066f2d4102987fad77bf8b24833fa94cc3dc4b33e0d7513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mimet.2010.08.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23371867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20826191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ahonsi, Monday O.</creatorcontrib><creatorcontrib>Ling, Yin</creatorcontrib><creatorcontrib>Kageyama, Koji</creatorcontrib><title>Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>Phytophthora nicotianae and
Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR.</description><subject>Biological and medical sciences</subject><subject>DNA, Fungal - chemistry</subject><subject>DNA, Fungal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Japan</subject><subject>Kalanchoe</subject><subject>Kalanchoe - microbiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multiplex PCR</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Mycology - methods</subject><subject>Oomycetes</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Phytophthora - genetics</subject><subject>Phytophthora - isolation & purification</subject><subject>Phytophthora nicotianae</subject><subject>Plant Diseases - microbiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Pythium</subject><subject>Pythium - genetics</subject><subject>Pythium - isolation & purification</subject><subject>Recycled irrigation</subject><subject>SCAR markers</subject><subject>Sequence Analysis, DNA</subject><subject>Sustainable disease management strategies</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxiMEokvhCZCQL4hTFv9JYufAoVqgRapEVeBsOfa48TaJF9sp2hfj-fBuFrjBaSzPbz7NfF9RvCR4TTBp3m7XoxshrSnOP1isMWkfFSsiOC0Fq9vHxSpTvOSY0LPiWYxbjEnNKvG0OKNY0Ia0ZFX8fA8PMPjdCFNC3qIvm4tbNKpwDyEiNRl0s7lFKka1j8j6gKKb7gZAx9c4D0lN4OeI4g60g1geq3UaGUigk_PTQfSm3ye_61Pvg0KT0z45NSlY9Pepd_OIehhywxmIyE0Iuq7M3dIO_gdyIbg7lcCgezWoSfcenhdPrBoivDjV8-Lbxw9fN1fl9efLT5uL61JXFKey4w0hbdM2jApta8MtbhpLTUUwbQW3ynDeWdHRSjBmVVtpzYyuOsYAG14Tdl68WXR3wX-fISY5uqhhGJa7pciG8qZq-H9JXreEES5wJtlC6uBjDGDlLrhs-V4SLA_Jyq08JisPyUosZE42T7066c_dCObPzO8oM_D6BKio1WBDdsrFvxxjnIjjou8WDrJvDw6CjDm6SYNxIUcmjXf_XOQX0vDF6g</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Ahonsi, Monday O.</creator><creator>Ling, Yin</creator><creator>Kageyama, Koji</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7T7</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20101101</creationdate><title>Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe</title><author>Ahonsi, Monday O. ; Ling, Yin ; Kageyama, Koji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-b76119696328cf5d7f066f2d4102987fad77bf8b24833fa94cc3dc4b33e0d7513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>DNA, Fungal - chemistry</topic><topic>DNA, Fungal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Japan</topic><topic>Kalanchoe</topic><topic>Kalanchoe - microbiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multiplex PCR</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Mycology - methods</topic><topic>Oomycetes</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Phytophthora - genetics</topic><topic>Phytophthora - isolation & purification</topic><topic>Phytophthora nicotianae</topic><topic>Plant Diseases - microbiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Pythium</topic><topic>Pythium - genetics</topic><topic>Pythium - isolation & purification</topic><topic>Recycled irrigation</topic><topic>SCAR markers</topic><topic>Sequence Analysis, DNA</topic><topic>Sustainable disease management strategies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ahonsi, Monday O.</creatorcontrib><creatorcontrib>Ling, Yin</creatorcontrib><creatorcontrib>Kageyama, Koji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of microbiological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ahonsi, Monday O.</au><au>Ling, Yin</au><au>Kageyama, Koji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe</atitle><jtitle>Journal of microbiological methods</jtitle><addtitle>J Microbiol Methods</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>83</volume><issue>2</issue><spage>260</spage><epage>265</epage><pages>260-265</pages><issn>0167-7012</issn><eissn>1872-8359</eissn><coden>JMIMDQ</coden><abstract>Phytophthora nicotianae and
Pythium helicoides are important water-borne oomycete pathogens of irrigated ornamentals particularly ebb-and-flow irrigated kalanchoe in Japan. We developed novel PCR-based sequence characterized amplified region markers and assays for rapid identification and species-specific detection of both pathogens in separate PCR reactions or simultaneously in a duplex PCR.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20826191</pmid><doi>10.1016/j.mimet.2010.08.019</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of microbiological methods, 2010-11, Vol.83 (2), p.260-265 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences DNA, Fungal - chemistry DNA, Fungal - genetics Fundamental and applied biological sciences. Psychology Fungal plant pathogens Japan Kalanchoe Kalanchoe - microbiology Microbiology Molecular Sequence Data Multiplex PCR Mycological methods and techniques used in mycology Mycology Mycology - methods Oomycetes Phytopathology. Animal pests. Plant and forest protection Phytophthora - genetics Phytophthora - isolation & purification Phytophthora nicotianae Plant Diseases - microbiology Polymerase Chain Reaction - methods Pythium Pythium - genetics Pythium - isolation & purification Recycled irrigation SCAR markers Sequence Analysis, DNA Sustainable disease management strategies |
| title | Development of SCAR markers and PCR assays for single or simultaneous species-specific detection of Phytophthora nicotianae and Pythium helicoides in ebb-and-flow irrigated kalanchoe |
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