Structure and sequence of the mouse Bmp6 gene

Inactivation of the Bmp5 and Bmp7 genes has provided preliminary insights into the developmental role of these proteins, with distinctive phenotypes of the mutant mice. We have begun to characterize the function of BMP-6 as a prototype factor of this subgroup. This cDNA was originally isolated from...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Mammalian genome 1997-03, Vol.8 (3), p.212-214
Hauptverfasser: Gitelman, S E, Kobrin, M, Lee, A, Fet, V, Lyons, K, Hogan, B L, Derynck, R
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 214
container_issue 3
container_start_page 212
container_title Mammalian genome
container_volume 8
creator Gitelman, S E
Kobrin, M
Lee, A
Fet, V
Lyons, K
Hogan, B L
Derynck, R
description Inactivation of the Bmp5 and Bmp7 genes has provided preliminary insights into the developmental role of these proteins, with distinctive phenotypes of the mutant mice. We have begun to characterize the function of BMP-6 as a prototype factor of this subgroup. This cDNA was originally isolated from a murine embryonic cDNA library by screening under low stringency with a Xenopus vg-1 cDNA probe, and the corresponding protein was named Vgr-1. cDNAs for the human and bovine homologs of Vgr-1 were subsequently isolated and were named BMP-6, although no bone morphogenetic activity was originally reported for this protein. Extensive in situ hybridization and immunohistochemical analyses have localized BMP-6 mRNA and protein expression in the central nervous system, suprabasal layer of the epidermis, and hypertrophic cartilage. We have overexpressed this factor in CHO cells and have shown that, when these cells are introduced subcutaneously into nude, athymic mice, the secreted BMP-6 protein induces ectopic cartilage and bone in a pattern that recapitulates endochondral bone formation. We have also overexpressed BMP-6 within a pluripotent mesenchymal cell line and have shown that the protein acts as an autocrine factor that induces osteoblastic differentiation in vitro. (DBO)
doi_str_mv 10.1007/s003359900391
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_853476237</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>853476237</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-c3642de7f0ceb57e4f8be886ba6249f1f25927f6e048826792e9e75937e2103c3</originalsourceid><addsrcrecordid>eNqFkb1PwzAQxS0EKqUwMiJZDDAF_H32CBVfUiUGYI4S9wxFTVLsZOC_x1UrJBhguuF-eu_dPUKOObvgjMFlYkxK7Vweju-QMVdSFBwAdsmYOWkLm3f75CCld8Y4GA4jMnLMOC7kmBRPfRx8P0SkVTunCT8GbD3SLtD-DWnTDQnpdbMy9BVbPCR7oVomPNrOCXm5vXme3hezx7uH6dWs8IpDX3hplJgjBOax1oAq2BqtNXVlhHKBB6GdgGCQKWuFASfQIWgnAQVn0ssJOd_ormKXA6W-bBbJ43JZtZgTlVZLBUZIyOTZnyTY7Cu1_RfkRgHTQmbw9Bf43g2xzeeWOajO33Vr22ID-dilFDGUq7hoqvhZclauayl_1JL5k63oUDc4_6a3PcgvnvGDwA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>792543297</pqid></control><display><type>article</type><title>Structure and sequence of the mouse Bmp6 gene</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Gitelman, S E ; Kobrin, M ; Lee, A ; Fet, V ; Lyons, K ; Hogan, B L ; Derynck, R</creator><creatorcontrib>Gitelman, S E ; Kobrin, M ; Lee, A ; Fet, V ; Lyons, K ; Hogan, B L ; Derynck, R</creatorcontrib><description>Inactivation of the Bmp5 and Bmp7 genes has provided preliminary insights into the developmental role of these proteins, with distinctive phenotypes of the mutant mice. We have begun to characterize the function of BMP-6 as a prototype factor of this subgroup. This cDNA was originally isolated from a murine embryonic cDNA library by screening under low stringency with a Xenopus vg-1 cDNA probe, and the corresponding protein was named Vgr-1. cDNAs for the human and bovine homologs of Vgr-1 were subsequently isolated and were named BMP-6, although no bone morphogenetic activity was originally reported for this protein. Extensive in situ hybridization and immunohistochemical analyses have localized BMP-6 mRNA and protein expression in the central nervous system, suprabasal layer of the epidermis, and hypertrophic cartilage. We have overexpressed this factor in CHO cells and have shown that, when these cells are introduced subcutaneously into nude, athymic mice, the secreted BMP-6 protein induces ectopic cartilage and bone in a pattern that recapitulates endochondral bone formation. We have also overexpressed BMP-6 within a pluripotent mesenchymal cell line and have shown that the protein acts as an autocrine factor that induces osteoblastic differentiation in vitro. (DBO)</description><identifier>ISSN: 0938-8990</identifier><identifier>EISSN: 1432-1777</identifier><identifier>DOI: 10.1007/s003359900391</identifier><identifier>PMID: 9069123</identifier><language>eng</language><publisher>United States: Springer Nature B.V</publisher><subject>Animals ; Binding Sites ; Bone Morphogenetic Protein 6 ; Bone Morphogenetic Proteins - genetics ; Exons ; Introns ; Mice ; Molecular Sequence Data ; Transcription Factors - metabolism</subject><ispartof>Mammalian genome, 1997-03, Vol.8 (3), p.212-214</ispartof><rights>Springcr-Verlag New York Inc 1997.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-c3642de7f0ceb57e4f8be886ba6249f1f25927f6e048826792e9e75937e2103c3</citedby><cites>FETCH-LOGICAL-c417t-c3642de7f0ceb57e4f8be886ba6249f1f25927f6e048826792e9e75937e2103c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9069123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gitelman, S E</creatorcontrib><creatorcontrib>Kobrin, M</creatorcontrib><creatorcontrib>Lee, A</creatorcontrib><creatorcontrib>Fet, V</creatorcontrib><creatorcontrib>Lyons, K</creatorcontrib><creatorcontrib>Hogan, B L</creatorcontrib><creatorcontrib>Derynck, R</creatorcontrib><title>Structure and sequence of the mouse Bmp6 gene</title><title>Mammalian genome</title><addtitle>Mamm Genome</addtitle><description>Inactivation of the Bmp5 and Bmp7 genes has provided preliminary insights into the developmental role of these proteins, with distinctive phenotypes of the mutant mice. We have begun to characterize the function of BMP-6 as a prototype factor of this subgroup. This cDNA was originally isolated from a murine embryonic cDNA library by screening under low stringency with a Xenopus vg-1 cDNA probe, and the corresponding protein was named Vgr-1. cDNAs for the human and bovine homologs of Vgr-1 were subsequently isolated and were named BMP-6, although no bone morphogenetic activity was originally reported for this protein. Extensive in situ hybridization and immunohistochemical analyses have localized BMP-6 mRNA and protein expression in the central nervous system, suprabasal layer of the epidermis, and hypertrophic cartilage. We have overexpressed this factor in CHO cells and have shown that, when these cells are introduced subcutaneously into nude, athymic mice, the secreted BMP-6 protein induces ectopic cartilage and bone in a pattern that recapitulates endochondral bone formation. We have also overexpressed BMP-6 within a pluripotent mesenchymal cell line and have shown that the protein acts as an autocrine factor that induces osteoblastic differentiation in vitro. (DBO)</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Bone Morphogenetic Protein 6</subject><subject>Bone Morphogenetic Proteins - genetics</subject><subject>Exons</subject><subject>Introns</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Transcription Factors - metabolism</subject><issn>0938-8990</issn><issn>1432-1777</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkb1PwzAQxS0EKqUwMiJZDDAF_H32CBVfUiUGYI4S9wxFTVLsZOC_x1UrJBhguuF-eu_dPUKOObvgjMFlYkxK7Vweju-QMVdSFBwAdsmYOWkLm3f75CCld8Y4GA4jMnLMOC7kmBRPfRx8P0SkVTunCT8GbD3SLtD-DWnTDQnpdbMy9BVbPCR7oVomPNrOCXm5vXme3hezx7uH6dWs8IpDX3hplJgjBOax1oAq2BqtNXVlhHKBB6GdgGCQKWuFASfQIWgnAQVn0ssJOd_ormKXA6W-bBbJ43JZtZgTlVZLBUZIyOTZnyTY7Cu1_RfkRgHTQmbw9Bf43g2xzeeWOajO33Vr22ID-dilFDGUq7hoqvhZclauayl_1JL5k63oUDc4_6a3PcgvnvGDwA</recordid><startdate>199703</startdate><enddate>199703</enddate><creator>Gitelman, S E</creator><creator>Kobrin, M</creator><creator>Lee, A</creator><creator>Fet, V</creator><creator>Lyons, K</creator><creator>Hogan, B L</creator><creator>Derynck, R</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199703</creationdate><title>Structure and sequence of the mouse Bmp6 gene</title><author>Gitelman, S E ; Kobrin, M ; Lee, A ; Fet, V ; Lyons, K ; Hogan, B L ; Derynck, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-c3642de7f0ceb57e4f8be886ba6249f1f25927f6e048826792e9e75937e2103c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Binding Sites</topic><topic>Bone Morphogenetic Protein 6</topic><topic>Bone Morphogenetic Proteins - genetics</topic><topic>Exons</topic><topic>Introns</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gitelman, S E</creatorcontrib><creatorcontrib>Kobrin, M</creatorcontrib><creatorcontrib>Lee, A</creatorcontrib><creatorcontrib>Fet, V</creatorcontrib><creatorcontrib>Lyons, K</creatorcontrib><creatorcontrib>Hogan, B L</creatorcontrib><creatorcontrib>Derynck, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Mammalian genome</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gitelman, S E</au><au>Kobrin, M</au><au>Lee, A</au><au>Fet, V</au><au>Lyons, K</au><au>Hogan, B L</au><au>Derynck, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and sequence of the mouse Bmp6 gene</atitle><jtitle>Mammalian genome</jtitle><addtitle>Mamm Genome</addtitle><date>1997-03</date><risdate>1997</risdate><volume>8</volume><issue>3</issue><spage>212</spage><epage>214</epage><pages>212-214</pages><issn>0938-8990</issn><eissn>1432-1777</eissn><abstract>Inactivation of the Bmp5 and Bmp7 genes has provided preliminary insights into the developmental role of these proteins, with distinctive phenotypes of the mutant mice. We have begun to characterize the function of BMP-6 as a prototype factor of this subgroup. This cDNA was originally isolated from a murine embryonic cDNA library by screening under low stringency with a Xenopus vg-1 cDNA probe, and the corresponding protein was named Vgr-1. cDNAs for the human and bovine homologs of Vgr-1 were subsequently isolated and were named BMP-6, although no bone morphogenetic activity was originally reported for this protein. Extensive in situ hybridization and immunohistochemical analyses have localized BMP-6 mRNA and protein expression in the central nervous system, suprabasal layer of the epidermis, and hypertrophic cartilage. We have overexpressed this factor in CHO cells and have shown that, when these cells are introduced subcutaneously into nude, athymic mice, the secreted BMP-6 protein induces ectopic cartilage and bone in a pattern that recapitulates endochondral bone formation. We have also overexpressed BMP-6 within a pluripotent mesenchymal cell line and have shown that the protein acts as an autocrine factor that induces osteoblastic differentiation in vitro. (DBO)</abstract><cop>United States</cop><pub>Springer Nature B.V</pub><pmid>9069123</pmid><doi>10.1007/s003359900391</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0938-8990
ispartof Mammalian genome, 1997-03, Vol.8 (3), p.212-214
issn 0938-8990
1432-1777
language eng
recordid cdi_proquest_miscellaneous_853476237
source MEDLINE; SpringerNature Journals
subjects Animals
Binding Sites
Bone Morphogenetic Protein 6
Bone Morphogenetic Proteins - genetics
Exons
Introns
Mice
Molecular Sequence Data
Transcription Factors - metabolism
title Structure and sequence of the mouse Bmp6 gene
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T21%3A00%3A39IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structure%20and%20sequence%20of%20the%20mouse%20Bmp6%20gene&rft.jtitle=Mammalian%20genome&rft.au=Gitelman,%20S%20E&rft.date=1997-03&rft.volume=8&rft.issue=3&rft.spage=212&rft.epage=214&rft.pages=212-214&rft.issn=0938-8990&rft.eissn=1432-1777&rft_id=info:doi/10.1007/s003359900391&rft_dat=%3Cproquest_cross%3E853476237%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=792543297&rft_id=info:pmid/9069123&rfr_iscdi=true