Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation
The nitrogen-fixing symbiont of alfalfa, Sinorhizobium meliloti, is able to use myo-inositol as the sole carbon source. Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this stud...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2010-12, Vol.76 (24), p.7972-7980 |
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| creator | Kohler, Petra R.A Zheng, Jasmine Y Schoffers, Elke Rossbach, Silvia |
| description | The nitrogen-fixing symbiont of alfalfa, Sinorhizobium meliloti, is able to use myo-inositol as the sole carbon source. Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this study, functional mutational analysis revealed that the iolA and iolCDEB genes are required for growth not only with the myo-isomer but also for growth with scyllo- and D-chiro-inositol as the sole carbon source. An additional, hypothetical dehydrogenase of the IdhA/MocA/GFO family encoded by the smc01163 gene was found to be essential for growth with scyllo-inositol, whereas the idhA-encoded myo-inositol dehydrogenase was responsible for the oxidation of D-chiro-inositol. The putative regulatory iolR gene, located upstream of iolCDEB, encodes a repressor of the iol genes, negatively regulating the activity of the myo- and the scyllo-inositol dehydrogenases. Mutants with insertions in the iolA, smc01163, and individual iolRCDE genes could not compete against the wild type in a nodule occupancy assay on alfalfa plants. Thus, a functional inositol catabolic pathway and its proper regulation are important nutritional or signaling factors in the S. meliloti-alfalfa symbiosis. |
| doi_str_mv | 10.1128/AEM.01972-10 |
| format | Article |
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Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this study, functional mutational analysis revealed that the iolA and iolCDEB genes are required for growth not only with the myo-isomer but also for growth with scyllo- and D-chiro-inositol as the sole carbon source. An additional, hypothetical dehydrogenase of the IdhA/MocA/GFO family encoded by the smc01163 gene was found to be essential for growth with scyllo-inositol, whereas the idhA-encoded myo-inositol dehydrogenase was responsible for the oxidation of D-chiro-inositol. The putative regulatory iolR gene, located upstream of iolCDEB, encodes a repressor of the iol genes, negatively regulating the activity of the myo- and the scyllo-inositol dehydrogenases. Mutants with insertions in the iolA, smc01163, and individual iolRCDE genes could not compete against the wild type in a nodule occupancy assay on alfalfa plants. Thus, a functional inositol catabolic pathway and its proper regulation are important nutritional or signaling factors in the S. meliloti-alfalfa symbiosis.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.01972-10</identifier><identifier>PMID: 20971862</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Bacillus subtilis ; Biological and medical sciences ; Carbon sources ; DNA Mutational Analysis ; DNA, Bacterial - genetics ; Flowers & plants ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Bacterial ; Genes, Bacterial ; Genomics ; Gram-positive bacteria ; Inositol - metabolism ; Medicago sativa - microbiology ; Metabolic Networks and Pathways - genetics ; Metabolism ; Microbiology ; Multigene Family ; Plant Microbiology ; Plant Root Nodulation ; Proteins ; Signal transduction ; Sinorhizobium meliloti ; Sinorhizobium meliloti - metabolism ; Sinorhizobium meliloti - physiology ; Symbiosis</subject><ispartof>Applied and Environmental Microbiology, 2010-12, Vol.76 (24), p.7972-7980</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Dec 2010</rights><rights>Copyright © 2010, American Society for Microbiology 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c598t-64c5b876911647784bc0bc7423ef7a4f98b92582867b00113a9922a5194cc4793</citedby><cites>FETCH-LOGICAL-c598t-64c5b876911647784bc0bc7423ef7a4f98b92582867b00113a9922a5194cc4793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008233/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3008233/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23635081$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20971862$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kohler, Petra R.A</creatorcontrib><creatorcontrib>Zheng, Jasmine Y</creatorcontrib><creatorcontrib>Schoffers, Elke</creatorcontrib><creatorcontrib>Rossbach, Silvia</creatorcontrib><title>Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The nitrogen-fixing symbiont of alfalfa, Sinorhizobium meliloti, is able to use myo-inositol as the sole carbon source. Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this study, functional mutational analysis revealed that the iolA and iolCDEB genes are required for growth not only with the myo-isomer but also for growth with scyllo- and D-chiro-inositol as the sole carbon source. An additional, hypothetical dehydrogenase of the IdhA/MocA/GFO family encoded by the smc01163 gene was found to be essential for growth with scyllo-inositol, whereas the idhA-encoded myo-inositol dehydrogenase was responsible for the oxidation of D-chiro-inositol. The putative regulatory iolR gene, located upstream of iolCDEB, encodes a repressor of the iol genes, negatively regulating the activity of the myo- and the scyllo-inositol dehydrogenases. Mutants with insertions in the iolA, smc01163, and individual iolRCDE genes could not compete against the wild type in a nodule occupancy assay on alfalfa plants. Thus, a functional inositol catabolic pathway and its proper regulation are important nutritional or signaling factors in the S. meliloti-alfalfa symbiosis.</description><subject>Bacillus subtilis</subject><subject>Biological and medical sciences</subject><subject>Carbon sources</subject><subject>DNA Mutational Analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Flowers & plants</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genes, Bacterial</subject><subject>Genomics</subject><subject>Gram-positive bacteria</subject><subject>Inositol - metabolism</subject><subject>Medicago sativa - microbiology</subject><subject>Metabolic Networks and Pathways - genetics</subject><subject>Metabolism</subject><subject>Microbiology</subject><subject>Multigene Family</subject><subject>Plant Microbiology</subject><subject>Plant Root Nodulation</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Sinorhizobium meliloti</subject><subject>Sinorhizobium meliloti - metabolism</subject><subject>Sinorhizobium meliloti - physiology</subject><subject>Symbiosis</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkstrVDEYxS-i2LG6c61BEDe9Ne_HRihDa4v1AbU7IeSmuTMpuTfTJLdl-tcbnbFVN7oKJD_O-XK-0zTPEdxHCMu3B4cf9yFSArcIPmhmCCrZMkL4w2YGoVItxhTuNE9yvoQQUsjl42YHQyWQ5HjWfDsZY_YlBjA3xXQx-DzsAQM-uDX4YsryxqyBH8GZH2Na-tvY-WkAgws-xOJBHxOYx2Hlii_-2oHjmAv4FC-mYIqP49PmUW9Cds-2525zfnT4dX7cnn5-fzI_OG0tU7K0nFrWScEVQpwKIWlnYWcFxcT1wtBeyU5hJrHkooMQIWKUwtgwpKi1VCiy27zb6K6mbnAX1o0lmaBXyQ8mrXU0Xv_5MvqlXsRrTSCUmJAq8GYrkOLV5HLRg8_WhWBGF6esFaO85krVP0nJCBWMyf8gEVOcbtxf_UVeximNNTEtcV0VkoRXaG8D2RRzTq6_-x6C-kcRdC2C_lmEelPxF79Hcgf_2nwFXm8Bk60JfTKj9fmeq5YMSnQ_3NIvljc-OW3yoI0btOAaUy2qYYVebqDeRG0WqQqdn2GISJ0HCYQp-Q4opM3c</recordid><startdate>20101201</startdate><enddate>20101201</enddate><creator>Kohler, Petra R.A</creator><creator>Zheng, Jasmine Y</creator><creator>Schoffers, Elke</creator><creator>Rossbach, Silvia</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20101201</creationdate><title>Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation</title><author>Kohler, Petra R.A ; Zheng, Jasmine Y ; Schoffers, Elke ; Rossbach, Silvia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c598t-64c5b876911647784bc0bc7423ef7a4f98b92582867b00113a9922a5194cc4793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Bacillus subtilis</topic><topic>Biological and medical sciences</topic><topic>Carbon sources</topic><topic>DNA Mutational Analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>Flowers & plants</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genes, Bacterial</topic><topic>Genomics</topic><topic>Gram-positive bacteria</topic><topic>Inositol - metabolism</topic><topic>Medicago sativa - microbiology</topic><topic>Metabolic Networks and Pathways - genetics</topic><topic>Metabolism</topic><topic>Microbiology</topic><topic>Multigene Family</topic><topic>Plant Microbiology</topic><topic>Plant Root Nodulation</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Sinorhizobium meliloti</topic><topic>Sinorhizobium meliloti - metabolism</topic><topic>Sinorhizobium meliloti - physiology</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kohler, Petra R.A</creatorcontrib><creatorcontrib>Zheng, Jasmine Y</creatorcontrib><creatorcontrib>Schoffers, Elke</creatorcontrib><creatorcontrib>Rossbach, Silvia</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kohler, Petra R.A</au><au>Zheng, Jasmine Y</au><au>Schoffers, Elke</au><au>Rossbach, Silvia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2010-12-01</date><risdate>2010</risdate><volume>76</volume><issue>24</issue><spage>7972</spage><epage>7980</epage><pages>7972-7980</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>The nitrogen-fixing symbiont of alfalfa, Sinorhizobium meliloti, is able to use myo-inositol as the sole carbon source. Putative inositol catabolism genes (iolA and iolRCDEB) have been identified in the S. meliloti genome based on their similarities with the Bacillus subtilis iol genes. In this study, functional mutational analysis revealed that the iolA and iolCDEB genes are required for growth not only with the myo-isomer but also for growth with scyllo- and D-chiro-inositol as the sole carbon source. An additional, hypothetical dehydrogenase of the IdhA/MocA/GFO family encoded by the smc01163 gene was found to be essential for growth with scyllo-inositol, whereas the idhA-encoded myo-inositol dehydrogenase was responsible for the oxidation of D-chiro-inositol. The putative regulatory iolR gene, located upstream of iolCDEB, encodes a repressor of the iol genes, negatively regulating the activity of the myo- and the scyllo-inositol dehydrogenases. Mutants with insertions in the iolA, smc01163, and individual iolRCDE genes could not compete against the wild type in a nodule occupancy assay on alfalfa plants. Thus, a functional inositol catabolic pathway and its proper regulation are important nutritional or signaling factors in the S. meliloti-alfalfa symbiosis.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>20971862</pmid><doi>10.1128/AEM.01972-10</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacillus subtilis Biological and medical sciences Carbon sources DNA Mutational Analysis DNA, Bacterial - genetics Flowers & plants Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genes, Bacterial Genomics Gram-positive bacteria Inositol - metabolism Medicago sativa - microbiology Metabolic Networks and Pathways - genetics Metabolism Microbiology Multigene Family Plant Microbiology Plant Root Nodulation Proteins Signal transduction Sinorhizobium meliloti Sinorhizobium meliloti - metabolism Sinorhizobium meliloti - physiology Symbiosis |
| title | Inositol Catabolism, a Key Pathway in Sinorhizobium meliloti for Competitive Host Nodulation |
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