External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study
Background: Circulating tumor cells (CTCs) are cells that have detached from solid tumors and entered the blood. CTCs can be detected, among others, by semi‐automated immunomagnetic enrichment and image cytometry using CellSearch® (Veridex, Raritan, NJ). We studied the feasibility of external qualit...
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| Veröffentlicht in: | Cytometry. Part B, Clinical cytometry Clinical cytometry, 2011-03, Vol.80B (2), p.112-118 |
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| container_title | Cytometry. Part B, Clinical cytometry |
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| creator | Kraan, Jaco Sleijfer, Stefan Strijbos, Michiel H. Ignatiadis, Michail Peeters, Dieter Pierga, Jean‐Yves Farace, Francoise Riethdorf, Sabine Fehm, Tanja Zorzino, Laura Tibbe, Arjan G. J. Maestro, Marisa Gisbert‐Criado, Rafael Denton, Graeme de Bono, Johann S. Dive, Caroline Foekens, John A. Gratama, Jan W. |
| description | Background:
Circulating tumor cells (CTCs) are cells that have detached from solid tumors and entered the blood. CTCs can be detected, among others, by semi‐automated immunomagnetic enrichment and image cytometry using CellSearch® (Veridex, Raritan, NJ). We studied the feasibility of external quality assurance (EQA) of the entire CellSearch procedure from blood draw to interpretation of results in multiple laboratories.
Methods:
Blood samples from six cancer patients and controls were distributed to 14 independent laboratories to test between‐laboratory, between‐assay, and between‐instrument variation. Additionally, between‐operator variability was assessed through the interpretation of blinded images of all blood samples on a website.
Results:
Shipment and storage of samples had no influence on CTC values. Between‐instrument (coefficient of variation (CV) < 12%) and between‐assay variation was low (CV ≤ 20%), indicating high reproducibility. However, between‐laboratory CV ranged from 45 to 64%. Although inter‐operator agreement on image interpretation (Fleiss' κ statistics) ranged from “substantial” to “almost perfect,” image interpretation, particularly of samples containing high numbers of apoptotic cells, was the main contributor to between‐laboratory variation.
Conclusions:
This multicenter study shows the feasibility of an EQA program for CTC detection in patient samples, and the importance of continuation of such a program for the harmonization of CTC enumeration. © 2010 International International Clinical Cytometry Society |
| doi_str_mv | 10.1002/cyto.b.20573 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_853470009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>853470009</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3343-6bb98601f3563957d119a2aa63e702ee1468a9d8b6e69f1e54e9c523be456b5a3</originalsourceid><addsrcrecordid>eNp9kLlOAzEQhi0EghDoqJE7GhLs9bExXbQKh4REQSioLNuZJYv2AHst2JfiIXgyNgcpqWY08-nTzI_QGSVjSkhy5bq2GdtxQkTK9tCACpGMuBLp_q7n6ggdh_BGCBNcpofoKKGMpVzRAQqzrxZ8bUr8EU1ZtB02IURvage4ybErvIulaYv6Fbexajx2UJYY6liB78dNjWNYL5eAs371BMa75c83Dl1oobrGU5yDCYUt1vLQxkV3gg5yUwY43dYher6ZzbO70cPj7X02fRg5xjgbSWvVRBKaMyFZ_9CCUmUSYySDlCQAlMuJUYuJlSBVTkFwUE4kzAIX0grDhuhi4333zUeE0OqqCKv7TQ1NDHoiGE8JIaonLzek800IHnL97ovK-E5Tolcp61XK2up1yj1-vhVHW8FiB__F2gNsA3wWJXT_ynT2Mn_caH8BGqKLzw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>853470009</pqid></control><display><type>article</type><title>External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study</title><source>MEDLINE</source><source>Wiley Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Kraan, Jaco ; Sleijfer, Stefan ; Strijbos, Michiel H. ; Ignatiadis, Michail ; Peeters, Dieter ; Pierga, Jean‐Yves ; Farace, Francoise ; Riethdorf, Sabine ; Fehm, Tanja ; Zorzino, Laura ; Tibbe, Arjan G. J. ; Maestro, Marisa ; Gisbert‐Criado, Rafael ; Denton, Graeme ; de Bono, Johann S. ; Dive, Caroline ; Foekens, John A. ; Gratama, Jan W.</creator><creatorcontrib>Kraan, Jaco ; Sleijfer, Stefan ; Strijbos, Michiel H. ; Ignatiadis, Michail ; Peeters, Dieter ; Pierga, Jean‐Yves ; Farace, Francoise ; Riethdorf, Sabine ; Fehm, Tanja ; Zorzino, Laura ; Tibbe, Arjan G. J. ; Maestro, Marisa ; Gisbert‐Criado, Rafael ; Denton, Graeme ; de Bono, Johann S. ; Dive, Caroline ; Foekens, John A. ; Gratama, Jan W.</creatorcontrib><description>Background:
Circulating tumor cells (CTCs) are cells that have detached from solid tumors and entered the blood. CTCs can be detected, among others, by semi‐automated immunomagnetic enrichment and image cytometry using CellSearch® (Veridex, Raritan, NJ). We studied the feasibility of external quality assurance (EQA) of the entire CellSearch procedure from blood draw to interpretation of results in multiple laboratories.
Methods:
Blood samples from six cancer patients and controls were distributed to 14 independent laboratories to test between‐laboratory, between‐assay, and between‐instrument variation. Additionally, between‐operator variability was assessed through the interpretation of blinded images of all blood samples on a website.
Results:
Shipment and storage of samples had no influence on CTC values. Between‐instrument (coefficient of variation (CV) < 12%) and between‐assay variation was low (CV ≤ 20%), indicating high reproducibility. However, between‐laboratory CV ranged from 45 to 64%. Although inter‐operator agreement on image interpretation (Fleiss' κ statistics) ranged from “substantial” to “almost perfect,” image interpretation, particularly of samples containing high numbers of apoptotic cells, was the main contributor to between‐laboratory variation.
Conclusions:
This multicenter study shows the feasibility of an EQA program for CTC detection in patient samples, and the importance of continuation of such a program for the harmonization of CTC enumeration. © 2010 International International Clinical Cytometry Society</description><identifier>ISSN: 1552-4949</identifier><identifier>EISSN: 1552-4957</identifier><identifier>DOI: 10.1002/cyto.b.20573</identifier><identifier>PMID: 21337491</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Cell Count ; circulating tumor cells ; external quality assessment ; Feasibility Studies ; Flow Cytometry - standards ; Humans ; immunomagnetic enrichment ; multicenter study ; Neoplasms - blood ; Neoplasms - pathology ; Neoplastic Cells, Circulating - pathology ; Quality Control ; rare events ; Reproducibility of Results</subject><ispartof>Cytometry. Part B, Clinical cytometry, 2011-03, Vol.80B (2), p.112-118</ispartof><rights>Copyright © 2010 International International Clinical Cytometry Society</rights><rights>Copyright © 2010 International International Clinical Cytometry Society.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3343-6bb98601f3563957d119a2aa63e702ee1468a9d8b6e69f1e54e9c523be456b5a3</citedby><cites>FETCH-LOGICAL-c3343-6bb98601f3563957d119a2aa63e702ee1468a9d8b6e69f1e54e9c523be456b5a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcyto.b.20573$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcyto.b.20573$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21337491$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kraan, Jaco</creatorcontrib><creatorcontrib>Sleijfer, Stefan</creatorcontrib><creatorcontrib>Strijbos, Michiel H.</creatorcontrib><creatorcontrib>Ignatiadis, Michail</creatorcontrib><creatorcontrib>Peeters, Dieter</creatorcontrib><creatorcontrib>Pierga, Jean‐Yves</creatorcontrib><creatorcontrib>Farace, Francoise</creatorcontrib><creatorcontrib>Riethdorf, Sabine</creatorcontrib><creatorcontrib>Fehm, Tanja</creatorcontrib><creatorcontrib>Zorzino, Laura</creatorcontrib><creatorcontrib>Tibbe, Arjan G. J.</creatorcontrib><creatorcontrib>Maestro, Marisa</creatorcontrib><creatorcontrib>Gisbert‐Criado, Rafael</creatorcontrib><creatorcontrib>Denton, Graeme</creatorcontrib><creatorcontrib>de Bono, Johann S.</creatorcontrib><creatorcontrib>Dive, Caroline</creatorcontrib><creatorcontrib>Foekens, John A.</creatorcontrib><creatorcontrib>Gratama, Jan W.</creatorcontrib><title>External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study</title><title>Cytometry. Part B, Clinical cytometry</title><addtitle>Cytometry B Clin Cytom</addtitle><description>Background:
Circulating tumor cells (CTCs) are cells that have detached from solid tumors and entered the blood. CTCs can be detected, among others, by semi‐automated immunomagnetic enrichment and image cytometry using CellSearch® (Veridex, Raritan, NJ). We studied the feasibility of external quality assurance (EQA) of the entire CellSearch procedure from blood draw to interpretation of results in multiple laboratories.
Methods:
Blood samples from six cancer patients and controls were distributed to 14 independent laboratories to test between‐laboratory, between‐assay, and between‐instrument variation. Additionally, between‐operator variability was assessed through the interpretation of blinded images of all blood samples on a website.
Results:
Shipment and storage of samples had no influence on CTC values. Between‐instrument (coefficient of variation (CV) < 12%) and between‐assay variation was low (CV ≤ 20%), indicating high reproducibility. However, between‐laboratory CV ranged from 45 to 64%. Although inter‐operator agreement on image interpretation (Fleiss' κ statistics) ranged from “substantial” to “almost perfect,” image interpretation, particularly of samples containing high numbers of apoptotic cells, was the main contributor to between‐laboratory variation.
Conclusions:
This multicenter study shows the feasibility of an EQA program for CTC detection in patient samples, and the importance of continuation of such a program for the harmonization of CTC enumeration. © 2010 International International Clinical Cytometry Society</description><subject>Cell Count</subject><subject>circulating tumor cells</subject><subject>external quality assessment</subject><subject>Feasibility Studies</subject><subject>Flow Cytometry - standards</subject><subject>Humans</subject><subject>immunomagnetic enrichment</subject><subject>multicenter study</subject><subject>Neoplasms - blood</subject><subject>Neoplasms - pathology</subject><subject>Neoplastic Cells, Circulating - pathology</subject><subject>Quality Control</subject><subject>rare events</subject><subject>Reproducibility of Results</subject><issn>1552-4949</issn><issn>1552-4957</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLlOAzEQhi0EghDoqJE7GhLs9bExXbQKh4REQSioLNuZJYv2AHst2JfiIXgyNgcpqWY08-nTzI_QGSVjSkhy5bq2GdtxQkTK9tCACpGMuBLp_q7n6ggdh_BGCBNcpofoKKGMpVzRAQqzrxZ8bUr8EU1ZtB02IURvage4ybErvIulaYv6Fbexajx2UJYY6liB78dNjWNYL5eAs371BMa75c83Dl1oobrGU5yDCYUt1vLQxkV3gg5yUwY43dYher6ZzbO70cPj7X02fRg5xjgbSWvVRBKaMyFZ_9CCUmUSYySDlCQAlMuJUYuJlSBVTkFwUE4kzAIX0grDhuhi4333zUeE0OqqCKv7TQ1NDHoiGE8JIaonLzek800IHnL97ovK-E5Tolcp61XK2up1yj1-vhVHW8FiB__F2gNsA3wWJXT_ynT2Mn_caH8BGqKLzw</recordid><startdate>201103</startdate><enddate>201103</enddate><creator>Kraan, Jaco</creator><creator>Sleijfer, Stefan</creator><creator>Strijbos, Michiel H.</creator><creator>Ignatiadis, Michail</creator><creator>Peeters, Dieter</creator><creator>Pierga, Jean‐Yves</creator><creator>Farace, Francoise</creator><creator>Riethdorf, Sabine</creator><creator>Fehm, Tanja</creator><creator>Zorzino, Laura</creator><creator>Tibbe, Arjan G. J.</creator><creator>Maestro, Marisa</creator><creator>Gisbert‐Criado, Rafael</creator><creator>Denton, Graeme</creator><creator>de Bono, Johann S.</creator><creator>Dive, Caroline</creator><creator>Foekens, John A.</creator><creator>Gratama, Jan W.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201103</creationdate><title>External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study</title><author>Kraan, Jaco ; Sleijfer, Stefan ; Strijbos, Michiel H. ; Ignatiadis, Michail ; Peeters, Dieter ; Pierga, Jean‐Yves ; Farace, Francoise ; Riethdorf, Sabine ; Fehm, Tanja ; Zorzino, Laura ; Tibbe, Arjan G. J. ; Maestro, Marisa ; Gisbert‐Criado, Rafael ; Denton, Graeme ; de Bono, Johann S. ; Dive, Caroline ; Foekens, John A. ; Gratama, Jan W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3343-6bb98601f3563957d119a2aa63e702ee1468a9d8b6e69f1e54e9c523be456b5a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Cell Count</topic><topic>circulating tumor cells</topic><topic>external quality assessment</topic><topic>Feasibility Studies</topic><topic>Flow Cytometry - standards</topic><topic>Humans</topic><topic>immunomagnetic enrichment</topic><topic>multicenter study</topic><topic>Neoplasms - blood</topic><topic>Neoplasms - pathology</topic><topic>Neoplastic Cells, Circulating - pathology</topic><topic>Quality Control</topic><topic>rare events</topic><topic>Reproducibility of Results</topic><toplevel>online_resources</toplevel><creatorcontrib>Kraan, Jaco</creatorcontrib><creatorcontrib>Sleijfer, Stefan</creatorcontrib><creatorcontrib>Strijbos, Michiel H.</creatorcontrib><creatorcontrib>Ignatiadis, Michail</creatorcontrib><creatorcontrib>Peeters, Dieter</creatorcontrib><creatorcontrib>Pierga, Jean‐Yves</creatorcontrib><creatorcontrib>Farace, Francoise</creatorcontrib><creatorcontrib>Riethdorf, Sabine</creatorcontrib><creatorcontrib>Fehm, Tanja</creatorcontrib><creatorcontrib>Zorzino, Laura</creatorcontrib><creatorcontrib>Tibbe, Arjan G. J.</creatorcontrib><creatorcontrib>Maestro, Marisa</creatorcontrib><creatorcontrib>Gisbert‐Criado, Rafael</creatorcontrib><creatorcontrib>Denton, Graeme</creatorcontrib><creatorcontrib>de Bono, Johann S.</creatorcontrib><creatorcontrib>Dive, Caroline</creatorcontrib><creatorcontrib>Foekens, John A.</creatorcontrib><creatorcontrib>Gratama, Jan W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytometry. Part B, Clinical cytometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kraan, Jaco</au><au>Sleijfer, Stefan</au><au>Strijbos, Michiel H.</au><au>Ignatiadis, Michail</au><au>Peeters, Dieter</au><au>Pierga, Jean‐Yves</au><au>Farace, Francoise</au><au>Riethdorf, Sabine</au><au>Fehm, Tanja</au><au>Zorzino, Laura</au><au>Tibbe, Arjan G. J.</au><au>Maestro, Marisa</au><au>Gisbert‐Criado, Rafael</au><au>Denton, Graeme</au><au>de Bono, Johann S.</au><au>Dive, Caroline</au><au>Foekens, John A.</au><au>Gratama, Jan W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study</atitle><jtitle>Cytometry. Part B, Clinical cytometry</jtitle><addtitle>Cytometry B Clin Cytom</addtitle><date>2011-03</date><risdate>2011</risdate><volume>80B</volume><issue>2</issue><spage>112</spage><epage>118</epage><pages>112-118</pages><issn>1552-4949</issn><eissn>1552-4957</eissn><abstract>Background:
Circulating tumor cells (CTCs) are cells that have detached from solid tumors and entered the blood. CTCs can be detected, among others, by semi‐automated immunomagnetic enrichment and image cytometry using CellSearch® (Veridex, Raritan, NJ). We studied the feasibility of external quality assurance (EQA) of the entire CellSearch procedure from blood draw to interpretation of results in multiple laboratories.
Methods:
Blood samples from six cancer patients and controls were distributed to 14 independent laboratories to test between‐laboratory, between‐assay, and between‐instrument variation. Additionally, between‐operator variability was assessed through the interpretation of blinded images of all blood samples on a website.
Results:
Shipment and storage of samples had no influence on CTC values. Between‐instrument (coefficient of variation (CV) < 12%) and between‐assay variation was low (CV ≤ 20%), indicating high reproducibility. However, between‐laboratory CV ranged from 45 to 64%. Although inter‐operator agreement on image interpretation (Fleiss' κ statistics) ranged from “substantial” to “almost perfect,” image interpretation, particularly of samples containing high numbers of apoptotic cells, was the main contributor to between‐laboratory variation.
Conclusions:
This multicenter study shows the feasibility of an EQA program for CTC detection in patient samples, and the importance of continuation of such a program for the harmonization of CTC enumeration. © 2010 International International Clinical Cytometry Society</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>21337491</pmid><doi>10.1002/cyto.b.20573</doi><tpages>7</tpages></addata></record> |
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| ispartof | Cytometry. Part B, Clinical cytometry, 2011-03, Vol.80B (2), p.112-118 |
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| source | MEDLINE; Wiley Free Content; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Cell Count circulating tumor cells external quality assessment Feasibility Studies Flow Cytometry - standards Humans immunomagnetic enrichment multicenter study Neoplasms - blood Neoplasms - pathology Neoplastic Cells, Circulating - pathology Quality Control rare events Reproducibility of Results |
| title | External quality assurance of circulating tumor cell enumeration using the CellSearch® system: A feasibility study |
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