NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma
Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. Recently...
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| Veröffentlicht in: | Tumor biology 2011-04, Vol.32 (2), p.359-366 |
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| creator | Peng, Zheng-Yu Xiao, Ping-Jie Qi, Yao Zhang, Wei Chen, Xian-Hua Xu, Ping |
| description | Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. Recently, NSSR1 has also been shown to be highly expressed in the testes, suggesting its potential roles in reproductive system. In this report, the expression of NSSR1 in the columnar epithelium of the endometrium and gland epithelium during the development of the mouse uterus, the regulation of NSSR1 level by testosterone in the adult mouse uterus, and expression level of NSSR1 in both human endometrial carcinomas and ovarian cancers were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry. We demonstrated that the expression of NSSR1 was developmentally regulated in the columnar epithelium of the endometrium and gland epithelium in the mouse uterus. Additionally, the NSSR1 level in the mouse uterus was maintained and regulated by testosterone. Interestingly, an enhanced level of NSSR1 was observed in both human endometrial carcinomas and ovarian cancers. Our results suggest that expression and distribution of NSSR1 is developmentally and hormonally regulated and up-regulated in endometrial carcinomas as well as ovarian cancers, indicating its potential involvement in uterine development and tumorgenesis. |
| doi_str_mv | 10.1007/s13277-010-0128-3 |
| format | Article |
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Recently, NSSR1 has also been shown to be highly expressed in the testes, suggesting its potential roles in reproductive system. In this report, the expression of NSSR1 in the columnar epithelium of the endometrium and gland epithelium during the development of the mouse uterus, the regulation of NSSR1 level by testosterone in the adult mouse uterus, and expression level of NSSR1 in both human endometrial carcinomas and ovarian cancers were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry. We demonstrated that the expression of NSSR1 was developmentally regulated in the columnar epithelium of the endometrium and gland epithelium in the mouse uterus. Additionally, the NSSR1 level in the mouse uterus was maintained and regulated by testosterone. Interestingly, an enhanced level of NSSR1 was observed in both human endometrial carcinomas and ovarian cancers. Our results suggest that expression and distribution of NSSR1 is developmentally and hormonally regulated and up-regulated in endometrial carcinomas as well as ovarian cancers, indicating its potential involvement in uterine development and tumorgenesis.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1007/s13277-010-0128-3</identifier><identifier>PMID: 21072693</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animals ; Biomarkers, Tumor - metabolism ; Biomedical and Life Sciences ; Biomedicine ; Cancer Research ; Case-Control Studies ; Cell Cycle Proteins - drug effects ; Cell Cycle Proteins - metabolism ; Cellular biology ; Endometrial cancer ; Endometrial Neoplasms - metabolism ; Endometrial Neoplasms - pathology ; Female ; Gene expression ; Gene Expression Regulation, Neoplastic ; Humans ; Mice ; Mice, Inbred Strains ; Models, Animal ; Neoplasm Proteins - drug effects ; Neoplasm Proteins - metabolism ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Proteins ; Repressor Proteins - drug effects ; Repressor Proteins - metabolism ; Research Article ; RNA-Binding Proteins - drug effects ; RNA-Binding Proteins - metabolism ; Rodents ; Serine-Arginine Splicing Factors ; Testosterone ; Testosterone - pharmacology ; Up-Regulation ; Uterus - metabolism</subject><ispartof>Tumor biology, 2011-04, Vol.32 (2), p.359-366</ispartof><rights>International Society of Oncology and BioMarkers (ISOBM) 2010</rights><rights>International Society of Oncology and BioMarkers (ISOBM) 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-1baf46d236b89f7ac22b98c7b0f791f514954cd8b1482d84cde4178b2631b9353</citedby><cites>FETCH-LOGICAL-c370t-1baf46d236b89f7ac22b98c7b0f791f514954cd8b1482d84cde4178b2631b9353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13277-010-0128-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13277-010-0128-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21072693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Zheng-Yu</creatorcontrib><creatorcontrib>Xiao, Ping-Jie</creatorcontrib><creatorcontrib>Qi, Yao</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Chen, Xian-Hua</creatorcontrib><creatorcontrib>Xu, Ping</creatorcontrib><title>NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma</title><title>Tumor biology</title><addtitle>Tumor Biol</addtitle><addtitle>Tumour Biol</addtitle><description>Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. 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Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Zheng-Yu</au><au>Xiao, Ping-Jie</au><au>Qi, Yao</au><au>Zhang, Wei</au><au>Chen, Xian-Hua</au><au>Xu, Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma</atitle><jtitle>Tumor biology</jtitle><stitle>Tumor Biol</stitle><addtitle>Tumour Biol</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>32</volume><issue>2</issue><spage>359</spage><epage>366</epage><pages>359-366</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><abstract>Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. Recently, NSSR1 has also been shown to be highly expressed in the testes, suggesting its potential roles in reproductive system. In this report, the expression of NSSR1 in the columnar epithelium of the endometrium and gland epithelium during the development of the mouse uterus, the regulation of NSSR1 level by testosterone in the adult mouse uterus, and expression level of NSSR1 in both human endometrial carcinomas and ovarian cancers were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry. We demonstrated that the expression of NSSR1 was developmentally regulated in the columnar epithelium of the endometrium and gland epithelium in the mouse uterus. Additionally, the NSSR1 level in the mouse uterus was maintained and regulated by testosterone. Interestingly, an enhanced level of NSSR1 was observed in both human endometrial carcinomas and ovarian cancers. Our results suggest that expression and distribution of NSSR1 is developmentally and hormonally regulated and up-regulated in endometrial carcinomas as well as ovarian cancers, indicating its potential involvement in uterine development and tumorgenesis.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>21072693</pmid><doi>10.1007/s13277-010-0128-3</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biomarkers, Tumor - metabolism Biomedical and Life Sciences Biomedicine Cancer Research Case-Control Studies Cell Cycle Proteins - drug effects Cell Cycle Proteins - metabolism Cellular biology Endometrial cancer Endometrial Neoplasms - metabolism Endometrial Neoplasms - pathology Female Gene expression Gene Expression Regulation, Neoplastic Humans Mice Mice, Inbred Strains Models, Animal Neoplasm Proteins - drug effects Neoplasm Proteins - metabolism Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Proteins Repressor Proteins - drug effects Repressor Proteins - metabolism Research Article RNA-Binding Proteins - drug effects RNA-Binding Proteins - metabolism Rodents Serine-Arginine Splicing Factors Testosterone Testosterone - pharmacology Up-Regulation Uterus - metabolism |
| title | NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma |
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