A Novel Capillary Electrophoresis-Based Multiplex PCR Assay for Detection of Respiratory Pathogens
The field of infectious disease testing has recently experienced rapid expansion in the number of multiplexed PCR-based assays available for detecting respiratory pathogens. This study provides a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the dete...
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| Veröffentlicht in: | Annals of clinical and laboratory science 2011, Vol.41 (1), p.33-38 |
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| container_title | Annals of clinical and laboratory science |
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| creator | STEVENSON, Jeffery B HYMAS, Weston C HILLYARD, David R |
| description | The field of infectious disease testing has recently experienced rapid expansion in the number of multiplexed PCR-based assays available for detecting respiratory pathogens. This study provides a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection platform for viral and bacterial respiratory pathogens. We compared this technology to a real-time PCR assay for 3 viral targets. Thirty respiratory samples were collected that had previously tested positive for either Flu A, Flu B, or RSV (ten of each). The Seegene assay detected 9/10 Flu A samples, 9/10 Flu B, and 10/10 RSV, for a total detection rate of 93%. The two samples that were undetected by the Seegene assay both generated late-crossing thresholds on the real-time platform, consistent with low viral loads. The Seeplex assay provides a promising alternative for multiplex respiratory testing. |
| format | Article |
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This study provides a preliminary evaluation of a multiplex assay from Seegene that uses capillary electrophoresis as the detection platform for viral and bacterial respiratory pathogens. We compared this technology to a real-time PCR assay for 3 viral targets. Thirty respiratory samples were collected that had previously tested positive for either Flu A, Flu B, or RSV (ten of each). The Seegene assay detected 9/10 Flu A samples, 9/10 Flu B, and 10/10 RSV, for a total detection rate of 93%. The two samples that were undetected by the Seegene assay both generated late-crossing thresholds on the real-time platform, consistent with low viral loads. The Seeplex assay provides a promising alternative for multiplex respiratory testing.</description><subject>Biological and medical sciences</subject><subject>Electrophoresis, Capillary - methods</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza B virus - genetics</subject><subject>Influenza B virus - isolation & purification</subject><subject>Medical sciences</subject><subject>Oligonucleotides - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Respiratory Syncytial Virus, Human - genetics</subject><subject>Respiratory Syncytial Virus, Human - isolation & purification</subject><subject>Viral diseases</subject><subject>Viral diseases of the respiratory system and ent viral diseases</subject><issn>0091-7370</issn><issn>1550-8080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1LxDAQBuAgiruu_gXJRTwVkjbptse1fsKqy6LnkqYTN5JtaqYV998bcUXmMJeHd3jngEy5lCwpWMEOyZSxkifzbM4m5ATxnbG0FIIdk0nKs1TGmZJmQZ_8Jzhaqd46p8KO3jjQQ_D9xgdAi8mVQmjp4-gG2zv4oqtqTReIakeND_Qahsit76g3dA3Y26AGH2NWatj4N-jwlBwZ5RDO9ntGXm9vXqr7ZPl891AtlkmfCjYkLZda5Dxvi5KXTEMpGJO64I0oRCZ1k7KshFzyNgXTzpssGpNrqZTg0oi8yWbk8je3D_5jBBzqrUUNsVQHfsS6kGnJfrKjPN_LsdlCW_fBbmPz-u8tEVzsgUKtnAmq0xb_XTxeyDzPvgEw1Wwg</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>STEVENSON, Jeffery B</creator><creator>HYMAS, Weston C</creator><creator>HILLYARD, David R</creator><general>Institute for Clinical Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2011</creationdate><title>A Novel Capillary Electrophoresis-Based Multiplex PCR Assay for Detection of Respiratory Pathogens</title><author>STEVENSON, Jeffery B ; HYMAS, Weston C ; HILLYARD, David R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p240t-d15c4616d89190ce94005c81b48435cb2039e651d2efd7b390cf6c5aa415f46b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Biological and medical sciences</topic><topic>Electrophoresis, Capillary - methods</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza B virus - genetics</topic><topic>Influenza B virus - isolation & purification</topic><topic>Medical sciences</topic><topic>Oligonucleotides - genetics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Respiratory Syncytial Virus, Human - genetics</topic><topic>Respiratory Syncytial Virus, Human - isolation & purification</topic><topic>Viral diseases</topic><topic>Viral diseases of the respiratory system and ent viral diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>STEVENSON, Jeffery B</creatorcontrib><creatorcontrib>HYMAS, Weston C</creatorcontrib><creatorcontrib>HILLYARD, David R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of clinical and laboratory science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>STEVENSON, Jeffery B</au><au>HYMAS, Weston C</au><au>HILLYARD, David R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Capillary Electrophoresis-Based Multiplex PCR Assay for Detection of Respiratory Pathogens</atitle><jtitle>Annals of clinical and laboratory science</jtitle><addtitle>Ann Clin Lab Sci</addtitle><date>2011</date><risdate>2011</risdate><volume>41</volume><issue>1</issue><spage>33</spage><epage>38</epage><pages>33-38</pages><issn>0091-7370</issn><eissn>1550-8080</eissn><coden>ACLSCP</coden><abstract>The field of infectious disease testing has recently experienced rapid expansion in the number of multiplexed PCR-based assays available for detecting respiratory pathogens. 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| fulltext | fulltext |
| identifier | ISSN: 0091-7370 |
| ispartof | Annals of clinical and laboratory science, 2011, Vol.41 (1), p.33-38 |
| issn | 0091-7370 1550-8080 |
| language | eng |
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| source | MEDLINE; EZB Electronic Journals Library; Journals@Ovid Complete |
| subjects | Biological and medical sciences Electrophoresis, Capillary - methods Human viral diseases Humans Infectious diseases Influenza A virus - genetics Influenza A virus - isolation & purification Influenza B virus - genetics Influenza B virus - isolation & purification Medical sciences Oligonucleotides - genetics Polymerase Chain Reaction - methods Respiratory Syncytial Virus, Human - genetics Respiratory Syncytial Virus, Human - isolation & purification Viral diseases Viral diseases of the respiratory system and ent viral diseases |
| title | A Novel Capillary Electrophoresis-Based Multiplex PCR Assay for Detection of Respiratory Pathogens |
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