TSLP from RSV-stimulated rat airway epithelial cells activates myeloid dendritic cells
The respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections in children, the elderly and in people who are immune suppressed, and is also the cause for the development of asthma primarily in infants. However, the immunological mechanisms by which RSV enhances aller...
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| Veröffentlicht in: | Immunology and cell biology 2011-02, Vol.89 (2), p.231-238 |
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| description | The respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections in children, the elderly and in people who are immune suppressed, and is also the cause for the development of asthma primarily in infants. However, the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aim of this study was to examine the influence of RSV‐infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells (mDCs).We found that the exposure of primary rat airway epithelial cells (PRAECs) to RSV induced a rapid (6 h), high (12 h) and persistent (18 h) increase in thymic stromal lymphopoietin (TSLP) mRNA compared with untreated PRAECs. TSLP protein expression was also enhanced by RSV infection. Functional maturation of mDCs was induced by RSV‐treated PRAECs, as shown by their enhanced levels of OX40L and thymus‐ and activation‐regulated chemokine (TARC) mRNAs, which increased the expressions of major histocompatibility complex II (MHCII) and CD86 costimulatory molecules and promoted enhanced T‐cell proliferation in mixed lymphocyte reactions. These activities were inhibited in cocultures with RSV‐infected RTECs (rat tracheal epithelial cells, an immortalized cell strain) that had been pretreated with TSLP‐targeted small interfering RNA. These results suggest that RSV can induce epithelial cells to produce TSLP, which in turn promotes the maturation of mDCs that might support Th2 cell polarization. |
| doi_str_mv | 10.1038/icb.2010.85 |
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However, the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aim of this study was to examine the influence of RSV‐infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells (mDCs).We found that the exposure of primary rat airway epithelial cells (PRAECs) to RSV induced a rapid (6 h), high (12 h) and persistent (18 h) increase in thymic stromal lymphopoietin (TSLP) mRNA compared with untreated PRAECs. TSLP protein expression was also enhanced by RSV infection. Functional maturation of mDCs was induced by RSV‐treated PRAECs, as shown by their enhanced levels of OX40L and thymus‐ and activation‐regulated chemokine (TARC) mRNAs, which increased the expressions of major histocompatibility complex II (MHCII) and CD86 costimulatory molecules and promoted enhanced T‐cell proliferation in mixed lymphocyte reactions. These activities were inhibited in cocultures with RSV‐infected RTECs (rat tracheal epithelial cells, an immortalized cell strain) that had been pretreated with TSLP‐targeted small interfering RNA. These results suggest that RSV can induce epithelial cells to produce TSLP, which in turn promotes the maturation of mDCs that might support Th2 cell polarization.</description><identifier>ISSN: 0818-9641</identifier><identifier>EISSN: 1440-1711</identifier><identifier>DOI: 10.1038/icb.2010.85</identifier><identifier>PMID: 20603637</identifier><language>eng</language><publisher>United States: Nature Publishing Group</publisher><subject>airway epithelial cells ; Animals ; asthma ; Biomarkers ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Coculture Techniques ; Cytokines - genetics ; Cytokines - metabolism ; dendritic cells ; Dendritic Cells - cytology ; Dendritic Cells - immunology ; Epithelial Cells - virology ; Myeloid Cells - cytology ; Rats ; Rats, Wistar ; respiratory syncytial virus ; Respiratory Syncytial Virus Infections - immunology ; Respiratory Syncytial Virus Infections - virology ; Respiratory Syncytial Viruses - physiology ; T helper type 2 ; Th1 Cells - cytology ; Th1 Cells - immunology ; Th2 Cells - cytology ; Th2 Cells - immunology ; thymic stromal lymphopoietin ; Toll-Like Receptor 3 - metabolism ; Trachea - cytology ; Up-Regulation</subject><ispartof>Immunology and cell biology, 2011-02, Vol.89 (2), p.231-238</ispartof><rights>2011 Australasian Society for Immunology Inc.</rights><rights>Copyright Nature Publishing Group Feb 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4505-8bf91594ee492e374f04138a2e45d45b77852d41cda042cd5b990f077f0c72943</citedby><cites>FETCH-LOGICAL-c4505-8bf91594ee492e374f04138a2e45d45b77852d41cda042cd5b990f077f0c72943</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1417,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20603637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiao, Jianou</creatorcontrib><creatorcontrib>Li, Ang</creatorcontrib><creatorcontrib>Jin, Xianqiao</creatorcontrib><title>TSLP from RSV-stimulated rat airway epithelial cells activates myeloid dendritic cells</title><title>Immunology and cell biology</title><addtitle>Immunol Cell Biol</addtitle><description>The respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections in children, the elderly and in people who are immune suppressed, and is also the cause for the development of asthma primarily in infants. However, the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aim of this study was to examine the influence of RSV‐infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells (mDCs).We found that the exposure of primary rat airway epithelial cells (PRAECs) to RSV induced a rapid (6 h), high (12 h) and persistent (18 h) increase in thymic stromal lymphopoietin (TSLP) mRNA compared with untreated PRAECs. TSLP protein expression was also enhanced by RSV infection. Functional maturation of mDCs was induced by RSV‐treated PRAECs, as shown by their enhanced levels of OX40L and thymus‐ and activation‐regulated chemokine (TARC) mRNAs, which increased the expressions of major histocompatibility complex II (MHCII) and CD86 costimulatory molecules and promoted enhanced T‐cell proliferation in mixed lymphocyte reactions. These activities were inhibited in cocultures with RSV‐infected RTECs (rat tracheal epithelial cells, an immortalized cell strain) that had been pretreated with TSLP‐targeted small interfering RNA. These results suggest that RSV can induce epithelial cells to produce TSLP, which in turn promotes the maturation of mDCs that might support Th2 cell polarization.</description><subject>airway epithelial cells</subject><subject>Animals</subject><subject>asthma</subject><subject>Biomarkers</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Coculture Techniques</subject><subject>Cytokines - genetics</subject><subject>Cytokines - metabolism</subject><subject>dendritic cells</subject><subject>Dendritic Cells - cytology</subject><subject>Dendritic Cells - immunology</subject><subject>Epithelial Cells - virology</subject><subject>Myeloid Cells - cytology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>respiratory syncytial virus</subject><subject>Respiratory Syncytial Virus Infections - immunology</subject><subject>Respiratory Syncytial Virus Infections - virology</subject><subject>Respiratory Syncytial Viruses - physiology</subject><subject>T helper type 2</subject><subject>Th1 Cells - cytology</subject><subject>Th1 Cells - immunology</subject><subject>Th2 Cells - cytology</subject><subject>Th2 Cells - immunology</subject><subject>thymic stromal lymphopoietin</subject><subject>Toll-Like Receptor 3 - metabolism</subject><subject>Trachea - cytology</subject><subject>Up-Regulation</subject><issn>0818-9641</issn><issn>1440-1711</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp90MFvFCEUBnBiNHatnrwriYcezNTHAAscdVNtk200tvZKGHgTaWZ2tjBjs_-9rLPtwUNPQPJ7Xx4fIW8ZnDLg-lP0zWkN5aXlM7JgQkDFFGPPyQI005VZCnZEXuV8CwCq1vwlOaphCXzJ1YLcXF-tf9A2DT39eXVT5TH2U-dGDDS5kbqY7t2O4jaOv7GLrqMeuy5T58f4p6hM-x12Qww04CakOEY_i9fkReu6jG8O5zH59fXsenVerb9_u1h9XldeSJCVblrDpBGIwtTIlWhBMK5djUIGIRultKyDYD44ELUPsjEGWlCqBa9qI_gxOZlzt2m4mzCPto95v4Hb4DBlW8YNlN_yIj_8J2-HKW3KcpYpvZRaaiOL-jgrn4acE7Z2m2Lv0s4ysPu2bWnb7tsu0UW_O2ROTY_h0T7UWwCfwX3scPdUlr24XH3Z3__Fvp-nNm6cEj6OFX4QfwGBIZNA</recordid><startdate>201102</startdate><enddate>201102</enddate><creator>Qiao, Jianou</creator><creator>Li, Ang</creator><creator>Jin, Xianqiao</creator><general>Nature Publishing Group</general><general>Blackwell Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201102</creationdate><title>TSLP from RSV-stimulated rat airway epithelial cells activates myeloid dendritic cells</title><author>Qiao, Jianou ; Li, Ang ; Jin, Xianqiao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4505-8bf91594ee492e374f04138a2e45d45b77852d41cda042cd5b990f077f0c72943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>airway epithelial cells</topic><topic>Animals</topic><topic>asthma</topic><topic>Biomarkers</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Coculture Techniques</topic><topic>Cytokines - genetics</topic><topic>Cytokines - metabolism</topic><topic>dendritic cells</topic><topic>Dendritic Cells - cytology</topic><topic>Dendritic Cells - immunology</topic><topic>Epithelial Cells - virology</topic><topic>Myeloid Cells - cytology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>respiratory syncytial virus</topic><topic>Respiratory Syncytial Virus Infections - immunology</topic><topic>Respiratory Syncytial Virus Infections - virology</topic><topic>Respiratory Syncytial Viruses - physiology</topic><topic>T helper type 2</topic><topic>Th1 Cells - cytology</topic><topic>Th1 Cells - immunology</topic><topic>Th2 Cells - cytology</topic><topic>Th2 Cells - immunology</topic><topic>thymic stromal lymphopoietin</topic><topic>Toll-Like Receptor 3 - metabolism</topic><topic>Trachea - cytology</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiao, Jianou</creatorcontrib><creatorcontrib>Li, Ang</creatorcontrib><creatorcontrib>Jin, Xianqiao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Immunology and cell biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiao, Jianou</au><au>Li, Ang</au><au>Jin, Xianqiao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TSLP from RSV-stimulated rat airway epithelial cells activates myeloid dendritic cells</atitle><jtitle>Immunology and cell biology</jtitle><addtitle>Immunol Cell Biol</addtitle><date>2011-02</date><risdate>2011</risdate><volume>89</volume><issue>2</issue><spage>231</spage><epage>238</epage><pages>231-238</pages><issn>0818-9641</issn><eissn>1440-1711</eissn><abstract>The respiratory syncytial virus (RSV) is a primary cause of lower respiratory tract infections in children, the elderly and in people who are immune suppressed, and is also the cause for the development of asthma primarily in infants. However, the immunological mechanisms by which RSV enhances allergic sensitization and asthma remain unclear. The aim of this study was to examine the influence of RSV‐infected airway epithelial cells on the activation and functions of rat myeloid dendritic cells (mDCs).We found that the exposure of primary rat airway epithelial cells (PRAECs) to RSV induced a rapid (6 h), high (12 h) and persistent (18 h) increase in thymic stromal lymphopoietin (TSLP) mRNA compared with untreated PRAECs. TSLP protein expression was also enhanced by RSV infection. Functional maturation of mDCs was induced by RSV‐treated PRAECs, as shown by their enhanced levels of OX40L and thymus‐ and activation‐regulated chemokine (TARC) mRNAs, which increased the expressions of major histocompatibility complex II (MHCII) and CD86 costimulatory molecules and promoted enhanced T‐cell proliferation in mixed lymphocyte reactions. These activities were inhibited in cocultures with RSV‐infected RTECs (rat tracheal epithelial cells, an immortalized cell strain) that had been pretreated with TSLP‐targeted small interfering RNA. These results suggest that RSV can induce epithelial cells to produce TSLP, which in turn promotes the maturation of mDCs that might support Th2 cell polarization.</abstract><cop>United States</cop><pub>Nature Publishing Group</pub><pmid>20603637</pmid><doi>10.1038/icb.2010.85</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | airway epithelial cells Animals asthma Biomarkers Cell Differentiation Cell Proliferation Cells, Cultured Coculture Techniques Cytokines - genetics Cytokines - metabolism dendritic cells Dendritic Cells - cytology Dendritic Cells - immunology Epithelial Cells - virology Myeloid Cells - cytology Rats Rats, Wistar respiratory syncytial virus Respiratory Syncytial Virus Infections - immunology Respiratory Syncytial Virus Infections - virology Respiratory Syncytial Viruses - physiology T helper type 2 Th1 Cells - cytology Th1 Cells - immunology Th2 Cells - cytology Th2 Cells - immunology thymic stromal lymphopoietin Toll-Like Receptor 3 - metabolism Trachea - cytology Up-Regulation |
| title | TSLP from RSV-stimulated rat airway epithelial cells activates myeloid dendritic cells |
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