Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation
Abstract Background aims Bone marrow- and adipose tissue-derived mesenchymal stromal cells (MSC) represent promising sources for regenerative medicine. However, the precise molecular mechanisms underlying MSC stemness maintenance versus differentiation are not fully understood. The aim of this study...
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| Veröffentlicht in: | Cytotherapy (Oxford, England) England), 2011-03, Vol.13 (3), p.329-340 |
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| creator | Saulnier, Nathalie Puglisi, Maria A Lattanzi, Wanda Castellini, Laura Pani, Giovambattista Leone, Giuseppe Alfieri, Sergio Michetti, Fabrizio Piscaglia, Anna Chiara Gasbarrini, Antonio |
| description | Abstract Background aims Bone marrow- and adipose tissue-derived mesenchymal stromal cells (MSC) represent promising sources for regenerative medicine. However, the precise molecular mechanisms underlying MSC stemness maintenance versus differentiation are not fully understood. The aim of this study was to compare the genome-wide expression profiles of bone marrow-and adipose tissue-derived MSC, in order to identify a common molecular stemness core. Methods Molecular profiling was carried out using Affymetrix microarray and relevant genes were further validated by Q-PCR. Results We identified an overlapping dataset of 190 transcripts commonly regulated in both cell populations, which included several genes involved in stemness regulation (i.e. self-renewal potential and the ability to generate differentiated cells), various signaling pathways and transcription factors. In particular, we identified a central role of the Kruppel-like factor 4 (KLF4) DNA-binding protein in regulating MSC transcriptional activity. Conclusions Our results provide new insights toward understanding the molecular basis of MSC stemness maintenance and underline the ability of KLF4 to maintain cells in an undifferentiated state. |
| doi_str_mv | 10.3109/14653249.2010.515576 |
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However, the precise molecular mechanisms underlying MSC stemness maintenance versus differentiation are not fully understood. The aim of this study was to compare the genome-wide expression profiles of bone marrow-and adipose tissue-derived MSC, in order to identify a common molecular stemness core. Methods Molecular profiling was carried out using Affymetrix microarray and relevant genes were further validated by Q-PCR. Results We identified an overlapping dataset of 190 transcripts commonly regulated in both cell populations, which included several genes involved in stemness regulation (i.e. self-renewal potential and the ability to generate differentiated cells), various signaling pathways and transcription factors. In particular, we identified a central role of the Kruppel-like factor 4 (KLF4) DNA-binding protein in regulating MSC transcriptional activity. Conclusions Our results provide new insights toward understanding the molecular basis of MSC stemness maintenance and underline the ability of KLF4 to maintain cells in an undifferentiated state.</description><identifier>ISSN: 1465-3249</identifier><identifier>EISSN: 1477-2566</identifier><identifier>DOI: 10.3109/14653249.2010.515576</identifier><identifier>PMID: 20849362</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Adipose Tissue - cytology ; adipose tissue stromal cells ; Adult ; Advanced Basic Science ; Binding Sites ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; bone marrow stromal cells ; Cell Differentiation - genetics ; Cell Lineage - genetics ; Chromatin Immunoprecipitation ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Gene Knockdown Techniques ; Humans ; Kruppel-like factor 4 ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; Male ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; microarray ; Middle Aged ; Models, Biological ; Other ; Polymerase Chain Reaction ; Promoter Regions, Genetic - genetics ; Protein Binding ; Reproducibility of Results ; Stromal Cells - cytology ; Stromal Cells - metabolism ; Young Adult</subject><ispartof>Cytotherapy (Oxford, England), 2011-03, Vol.13 (3), p.329-340</ispartof><rights>International Society for Cellular Therapy</rights><rights>2011 International Society for Cellular Therapy</rights><rights>2011 Informa Healthcare 2011</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-28abddd204fc2e72316b6183026b28719b397353e4d03a86a90fa9ed89f5c3a03</citedby><cites>FETCH-LOGICAL-c471t-28abddd204fc2e72316b6183026b28719b397353e4d03a86a90fa9ed89f5c3a03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20849362$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saulnier, Nathalie</creatorcontrib><creatorcontrib>Puglisi, Maria A</creatorcontrib><creatorcontrib>Lattanzi, Wanda</creatorcontrib><creatorcontrib>Castellini, Laura</creatorcontrib><creatorcontrib>Pani, Giovambattista</creatorcontrib><creatorcontrib>Leone, Giuseppe</creatorcontrib><creatorcontrib>Alfieri, Sergio</creatorcontrib><creatorcontrib>Michetti, Fabrizio</creatorcontrib><creatorcontrib>Piscaglia, Anna Chiara</creatorcontrib><creatorcontrib>Gasbarrini, Antonio</creatorcontrib><title>Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation</title><title>Cytotherapy (Oxford, England)</title><addtitle>Cytotherapy</addtitle><description>Abstract Background aims Bone marrow- and adipose tissue-derived mesenchymal stromal cells (MSC) represent promising sources for regenerative medicine. However, the precise molecular mechanisms underlying MSC stemness maintenance versus differentiation are not fully understood. The aim of this study was to compare the genome-wide expression profiles of bone marrow-and adipose tissue-derived MSC, in order to identify a common molecular stemness core. Methods Molecular profiling was carried out using Affymetrix microarray and relevant genes were further validated by Q-PCR. Results We identified an overlapping dataset of 190 transcripts commonly regulated in both cell populations, which included several genes involved in stemness regulation (i.e. self-renewal potential and the ability to generate differentiated cells), various signaling pathways and transcription factors. In particular, we identified a central role of the Kruppel-like factor 4 (KLF4) DNA-binding protein in regulating MSC transcriptional activity. Conclusions Our results provide new insights toward understanding the molecular basis of MSC stemness maintenance and underline the ability of KLF4 to maintain cells in an undifferentiated state.</description><subject>Adipose Tissue - cytology</subject><subject>adipose tissue stromal cells</subject><subject>Adult</subject><subject>Advanced Basic Science</subject><subject>Binding Sites</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>bone marrow stromal cells</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Lineage - genetics</subject><subject>Chromatin Immunoprecipitation</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Gene Knockdown Techniques</subject><subject>Humans</subject><subject>Kruppel-like factor 4</subject><subject>Kruppel-Like Transcription Factors - genetics</subject><subject>Kruppel-Like Transcription Factors - metabolism</subject><subject>Male</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>microarray</subject><subject>Middle Aged</subject><subject>Models, Biological</subject><subject>Other</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protein Binding</subject><subject>Reproducibility of Results</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - metabolism</subject><subject>Young Adult</subject><issn>1465-3249</issn><issn>1477-2566</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctu1TAQhi0Eohd4A4S8Y5XiS-zELJBQBS2iEgtgbTn2pHXrxMF2irrnwXE458ASsZrR6J9_Zr5B6AUlZ5wS9Zq2UnDWqjNGaklQITr5CB3TtusaJqR8vOVSNJvmCJ3kfEsII30vnqKjGlvFJTtGPy9gBrykOPrg52scRzzEWplMSvFHg83ssHF-iRlw8Tmv0DhI_h4cziXFyQRsIYT8Bht8Bw84xQCbyae0LguEJvg7wKOxJSbcYj__VtdCAZzgeg2m-Dg_Q09GEzI838dT9O3D-6_nl83V54uP5--uGtt2tDSsN4NzjpF2tAw6xqkcJO05YXJgfUfVwFXHBYfWEW56aRQZjQLXq1FYbgg_Ra92vvXe7yvkoieft4XMDHHNuhdUcdF3m7LdKW2KOScY9ZJ8ZfKgKdEbfn3Arzf8eoe_tr3cD1iHCdyfpgPvKni7E_h5jGkyN2BCubEmgb6Na5rr9f-asDeAyuneQ9LZepgtOJ_AFu2i_18DWx_vrQn1fZD_bqEz00R_OZhQ2hFBlOS_APZ_vD8</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Saulnier, Nathalie</creator><creator>Puglisi, Maria A</creator><creator>Lattanzi, Wanda</creator><creator>Castellini, Laura</creator><creator>Pani, Giovambattista</creator><creator>Leone, Giuseppe</creator><creator>Alfieri, Sergio</creator><creator>Michetti, Fabrizio</creator><creator>Piscaglia, Anna Chiara</creator><creator>Gasbarrini, Antonio</creator><general>Elsevier Inc</general><general>Informa Healthcare</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110301</creationdate><title>Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation</title><author>Saulnier, Nathalie ; Puglisi, Maria A ; Lattanzi, Wanda ; Castellini, Laura ; Pani, Giovambattista ; Leone, Giuseppe ; Alfieri, Sergio ; Michetti, Fabrizio ; Piscaglia, Anna Chiara ; Gasbarrini, Antonio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-28abddd204fc2e72316b6183026b28719b397353e4d03a86a90fa9ed89f5c3a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adipose Tissue - cytology</topic><topic>adipose tissue stromal cells</topic><topic>Adult</topic><topic>Advanced Basic Science</topic><topic>Binding Sites</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>bone marrow stromal cells</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Lineage - genetics</topic><topic>Chromatin Immunoprecipitation</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Gene Knockdown Techniques</topic><topic>Humans</topic><topic>Kruppel-like factor 4</topic><topic>Kruppel-Like Transcription Factors - genetics</topic><topic>Kruppel-Like Transcription Factors - metabolism</topic><topic>Male</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>microarray</topic><topic>Middle Aged</topic><topic>Models, Biological</topic><topic>Other</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Protein Binding</topic><topic>Reproducibility of Results</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saulnier, Nathalie</creatorcontrib><creatorcontrib>Puglisi, Maria A</creatorcontrib><creatorcontrib>Lattanzi, Wanda</creatorcontrib><creatorcontrib>Castellini, Laura</creatorcontrib><creatorcontrib>Pani, Giovambattista</creatorcontrib><creatorcontrib>Leone, Giuseppe</creatorcontrib><creatorcontrib>Alfieri, Sergio</creatorcontrib><creatorcontrib>Michetti, Fabrizio</creatorcontrib><creatorcontrib>Piscaglia, Anna Chiara</creatorcontrib><creatorcontrib>Gasbarrini, Antonio</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytotherapy (Oxford, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saulnier, Nathalie</au><au>Puglisi, Maria A</au><au>Lattanzi, Wanda</au><au>Castellini, Laura</au><au>Pani, Giovambattista</au><au>Leone, Giuseppe</au><au>Alfieri, Sergio</au><au>Michetti, Fabrizio</au><au>Piscaglia, Anna Chiara</au><au>Gasbarrini, Antonio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation</atitle><jtitle>Cytotherapy (Oxford, England)</jtitle><addtitle>Cytotherapy</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>13</volume><issue>3</issue><spage>329</spage><epage>340</epage><pages>329-340</pages><issn>1465-3249</issn><eissn>1477-2566</eissn><abstract>Abstract Background aims Bone marrow- and adipose tissue-derived mesenchymal stromal cells (MSC) represent promising sources for regenerative medicine. However, the precise molecular mechanisms underlying MSC stemness maintenance versus differentiation are not fully understood. The aim of this study was to compare the genome-wide expression profiles of bone marrow-and adipose tissue-derived MSC, in order to identify a common molecular stemness core. Methods Molecular profiling was carried out using Affymetrix microarray and relevant genes were further validated by Q-PCR. Results We identified an overlapping dataset of 190 transcripts commonly regulated in both cell populations, which included several genes involved in stemness regulation (i.e. self-renewal potential and the ability to generate differentiated cells), various signaling pathways and transcription factors. In particular, we identified a central role of the Kruppel-like factor 4 (KLF4) DNA-binding protein in regulating MSC transcriptional activity. Conclusions Our results provide new insights toward understanding the molecular basis of MSC stemness maintenance and underline the ability of KLF4 to maintain cells in an undifferentiated state.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>20849362</pmid><doi>10.3109/14653249.2010.515576</doi><tpages>12</tpages></addata></record> |
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| subjects | Adipose Tissue - cytology adipose tissue stromal cells Adult Advanced Basic Science Binding Sites Bone Marrow Cells - cytology Bone Marrow Cells - metabolism bone marrow stromal cells Cell Differentiation - genetics Cell Lineage - genetics Chromatin Immunoprecipitation Female Gene Expression Profiling Gene Expression Regulation Gene Knockdown Techniques Humans Kruppel-like factor 4 Kruppel-Like Transcription Factors - genetics Kruppel-Like Transcription Factors - metabolism Male Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism microarray Middle Aged Models, Biological Other Polymerase Chain Reaction Promoter Regions, Genetic - genetics Protein Binding Reproducibility of Results Stromal Cells - cytology Stromal Cells - metabolism Young Adult |
| title | Gene profiling of bone marrow- and adipose tissue-derived stromal cells: a key role of Kruppel-like factor 4 in cell fate regulation |
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