Quality assessment of induced spermatogenesis in hypogonadotrophic hypogonadic men treated with gonadotrophins
Abstract Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenesis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integr...
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| creator | Krabchi, K Berthaut, I Chantot-Bastaraud, S Ravel, C Chabbert-Buffet, N de Larouzière, V Bouchard, P Mandelbaum, J Siffroi, J.-P Christin-Maitre, S |
| description | Abstract Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenesis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared with sperm samples from normospermic control donors. Sperm cells were analysed by fluorescence in-situ hybridization, using probes specific for chromosomes 13, 21, 18, X and Y, and by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients. Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenensis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve a paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared to sperm samples from normospermic control donors. Sperm cells were analysed by FISH, using probes specific for chromosomes 13, 21, 18, X and Y, and by TUNEL assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion n |
| doi_str_mv | 10.1016/j.rbmo.2010.11.017 |
| format | Article |
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In order to induce spermatogenesis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared with sperm samples from normospermic control donors. Sperm cells were analysed by fluorescence in-situ hybridization, using probes specific for chromosomes 13, 21, 18, X and Y, and by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients. Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenensis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve a paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared to sperm samples from normospermic control donors. Sperm cells were analysed by FISH, using probes specific for chromosomes 13, 21, 18, X and Y, and by TUNEL assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients.</description><identifier>ISSN: 1472-6483</identifier><identifier>EISSN: 1472-6491</identifier><identifier>DOI: 10.1016/j.rbmo.2010.11.017</identifier><identifier>PMID: 21269879</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>chromosomes ; Chromosomes, Human - ultrastructure ; DNA fragmentation ; FISH ; Gonadotropins - pharmacology ; Gonadotropins - therapeutic use ; Humans ; Hypogonadism - drug therapy ; hypogonadotrophic hypogonadism ; In Situ Hybridization, Fluorescence ; In Situ Nick-End Labeling ; induced spermatogenesis ; Male ; Obstetrics and Gynecology ; Semen Analysis - statistics & numerical data ; Sex Ratio ; Spermatogenesis - drug effects ; Spermatogenesis - physiology ; TUNEL assay</subject><ispartof>Reproductive biomedicine online, 2011-03, Vol.22 (3), p.277-283</ispartof><rights>Reproductive Healthcare Ltd.</rights><rights>2010 Reproductive Healthcare Ltd.</rights><rights>Copyright © 2010 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-6a5b0b1de7a88e5f4ada2ad495c3f072d0b8ad313da3c38d8a1864c4f9e3a17f3</citedby><cites>FETCH-LOGICAL-c454t-6a5b0b1de7a88e5f4ada2ad495c3f072d0b8ad313da3c38d8a1864c4f9e3a17f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rbmo.2010.11.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21269879$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Krabchi, K</creatorcontrib><creatorcontrib>Berthaut, I</creatorcontrib><creatorcontrib>Chantot-Bastaraud, S</creatorcontrib><creatorcontrib>Ravel, C</creatorcontrib><creatorcontrib>Chabbert-Buffet, N</creatorcontrib><creatorcontrib>de Larouzière, V</creatorcontrib><creatorcontrib>Bouchard, P</creatorcontrib><creatorcontrib>Mandelbaum, J</creatorcontrib><creatorcontrib>Siffroi, J.-P</creatorcontrib><creatorcontrib>Christin-Maitre, S</creatorcontrib><title>Quality assessment of induced spermatogenesis in hypogonadotrophic hypogonadic men treated with gonadotrophins</title><title>Reproductive biomedicine online</title><addtitle>Reprod Biomed Online</addtitle><description>Abstract Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenesis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared with sperm samples from normospermic control donors. Sperm cells were analysed by fluorescence in-situ hybridization, using probes specific for chromosomes 13, 21, 18, X and Y, and by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients. Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenensis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve a paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared to sperm samples from normospermic control donors. Sperm cells were analysed by FISH, using probes specific for chromosomes 13, 21, 18, X and Y, and by TUNEL assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients.</description><subject>chromosomes</subject><subject>Chromosomes, Human - ultrastructure</subject><subject>DNA fragmentation</subject><subject>FISH</subject><subject>Gonadotropins - pharmacology</subject><subject>Gonadotropins - therapeutic use</subject><subject>Humans</subject><subject>Hypogonadism - drug therapy</subject><subject>hypogonadotrophic hypogonadism</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>In Situ Nick-End Labeling</subject><subject>induced spermatogenesis</subject><subject>Male</subject><subject>Obstetrics and Gynecology</subject><subject>Semen Analysis - statistics & numerical data</subject><subject>Sex Ratio</subject><subject>Spermatogenesis - drug effects</subject><subject>Spermatogenesis - physiology</subject><subject>TUNEL assay</subject><issn>1472-6483</issn><issn>1472-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2LFDEQxRtR3A_9BzxI3zzNmEr6Iw0iyKKusCCy6zmkk-qdjN1Jm0or89-bZtZV9rCnKh7vPahfFcUrYFtg0Lzdb2M_hS1nqwBbBu2T4hSqlm-aqoOn97sUJ8UZ0Z4xkEyK58UJB950su1OC_9t0aNLh1ITIdGEPpVhKJ23i0Fb0oxx0incokdylPVyd5jDbfDahhTDvHPmn5L3XFCmiDrl8G-XduX_Vk8vimeDHglf3s3z4vunjzcXl5urr5-_XHy42piqrtKm0XXPerDYaimxHiptNde26mojBtZyy3qprQBhtTBCWqlBNpWphg6FhnYQ58WbY-8cw88FKanJkcFx1B7DQkrW0Na8akV28qPTxEAUcVBzdJOOBwVMrZjVXq2Y1YpZAaiMOYde39Uv_YT2PvKXaza8OxowH_nLYVRkHPqM1EU0SdngHu9__yBuRued0eMPPCDtwxJ9xqdAEVdMXa-PXv8MjLFWCi7-AJuCp1M</recordid><startdate>20110301</startdate><enddate>20110301</enddate><creator>Krabchi, K</creator><creator>Berthaut, I</creator><creator>Chantot-Bastaraud, S</creator><creator>Ravel, C</creator><creator>Chabbert-Buffet, N</creator><creator>de Larouzière, V</creator><creator>Bouchard, P</creator><creator>Mandelbaum, J</creator><creator>Siffroi, J.-P</creator><creator>Christin-Maitre, S</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110301</creationdate><title>Quality assessment of induced spermatogenesis in hypogonadotrophic hypogonadic men treated with gonadotrophins</title><author>Krabchi, K ; Berthaut, I ; Chantot-Bastaraud, S ; Ravel, C ; Chabbert-Buffet, N ; de Larouzière, V ; Bouchard, P ; Mandelbaum, J ; Siffroi, J.-P ; Christin-Maitre, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-6a5b0b1de7a88e5f4ada2ad495c3f072d0b8ad313da3c38d8a1864c4f9e3a17f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>chromosomes</topic><topic>Chromosomes, Human - ultrastructure</topic><topic>DNA fragmentation</topic><topic>FISH</topic><topic>Gonadotropins - pharmacology</topic><topic>Gonadotropins - therapeutic use</topic><topic>Humans</topic><topic>Hypogonadism - drug therapy</topic><topic>hypogonadotrophic hypogonadism</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>In Situ Nick-End Labeling</topic><topic>induced spermatogenesis</topic><topic>Male</topic><topic>Obstetrics and Gynecology</topic><topic>Semen Analysis - statistics & numerical data</topic><topic>Sex Ratio</topic><topic>Spermatogenesis - drug effects</topic><topic>Spermatogenesis - physiology</topic><topic>TUNEL assay</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krabchi, K</creatorcontrib><creatorcontrib>Berthaut, I</creatorcontrib><creatorcontrib>Chantot-Bastaraud, S</creatorcontrib><creatorcontrib>Ravel, C</creatorcontrib><creatorcontrib>Chabbert-Buffet, N</creatorcontrib><creatorcontrib>de Larouzière, V</creatorcontrib><creatorcontrib>Bouchard, P</creatorcontrib><creatorcontrib>Mandelbaum, J</creatorcontrib><creatorcontrib>Siffroi, J.-P</creatorcontrib><creatorcontrib>Christin-Maitre, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive biomedicine online</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krabchi, K</au><au>Berthaut, I</au><au>Chantot-Bastaraud, S</au><au>Ravel, C</au><au>Chabbert-Buffet, N</au><au>de Larouzière, V</au><au>Bouchard, P</au><au>Mandelbaum, J</au><au>Siffroi, J.-P</au><au>Christin-Maitre, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quality assessment of induced spermatogenesis in hypogonadotrophic hypogonadic men treated with gonadotrophins</atitle><jtitle>Reproductive biomedicine online</jtitle><addtitle>Reprod Biomed Online</addtitle><date>2011-03-01</date><risdate>2011</risdate><volume>22</volume><issue>3</issue><spage>277</spage><epage>283</epage><pages>277-283</pages><issn>1472-6483</issn><eissn>1472-6491</eissn><abstract>Abstract Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenesis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared with sperm samples from normospermic control donors. Sperm cells were analysed by fluorescence in-situ hybridization, using probes specific for chromosomes 13, 21, 18, X and Y, and by TdT (terminal deoxynucleotidyl transferase)-mediated dUDP nick-end labelling assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients. Hypogonadotrophic hypogonadism (HH) is characterized by deficient gonadotrophin secretion, resulting from pituitary or hypothalamic defects. In order to induce spermatogenensis, HH patients are treated with commercially available gonadotrophins. As far as is known, quality and genetic integrity of induced sperm cells have never been investigated, although they represent an important issue, since the ultimate goal of this treatment is to have competent spermatozoa in order to achieve a paternity. In order to evaluate the nuclear integrity of induced sperm cells, sperm samples from treated HH patients were compared to sperm samples from normospermic control donors. Sperm cells were analysed by FISH, using probes specific for chromosomes 13, 21, 18, X and Y, and by TUNEL assay. Results showed that the rate of aneuploid and diploid sperm cells in patients was not statistically different from controls and that the rate of sperm cells with fragmented DNA was within the normal values. Spermatozoa obtained by gonadotrophin treatment in HH patients are likely to have a balanced chromosomal content and a normal DNA integrity but this conclusion needs to be confirmed by further studies dealing with a greater number of patients.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>21269879</pmid><doi>10.1016/j.rbmo.2010.11.017</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | chromosomes Chromosomes, Human - ultrastructure DNA fragmentation FISH Gonadotropins - pharmacology Gonadotropins - therapeutic use Humans Hypogonadism - drug therapy hypogonadotrophic hypogonadism In Situ Hybridization, Fluorescence In Situ Nick-End Labeling induced spermatogenesis Male Obstetrics and Gynecology Semen Analysis - statistics & numerical data Sex Ratio Spermatogenesis - drug effects Spermatogenesis - physiology TUNEL assay |
| title | Quality assessment of induced spermatogenesis in hypogonadotrophic hypogonadic men treated with gonadotrophins |
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