Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography
An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and...
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| Veröffentlicht in: | Journal of AOAC International 2010-11, Vol.93 (6), p.1897-1904 |
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| container_issue | 6 |
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| container_title | Journal of AOAC International |
| container_volume | 93 |
| creator | ELLINGSON, David POTTS, Brian ANDERSON, Phillip BURKHARDT, Greg ELLEFSON, Wayne SULLIVAN, Darryl JACOBS, Wesley RAGAN, Robert |
| description | An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0% across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4%, respectively. Overall average recovery was 99.4% (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106% (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages. |
| doi_str_mv | 10.1093/jaoac/93.6.1897 |
| format | Article |
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The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0% across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4%, respectively. Overall average recovery was 99.4% (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106% (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.1093/jaoac/93.6.1897</identifier><identifier>PMID: 21313818</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>Analysis ; Anion Exchange Resins ; Biological and medical sciences ; Calibration ; Carbohydrates ; Chromatography, Ion Exchange ; Dietary Carbohydrates - analysis ; Food industries ; Fundamental and applied biological sciences. Psychology ; Galactose ; High performance liquid chromatography ; Humans ; Indicators and Reagents ; Infant ; Infant Food - analysis ; Low carbohydrate foods ; Physiological aspects ; Reference Standards ; Reproducibility of Results ; Solutions ; Starch - analysis</subject><ispartof>Journal of AOAC International, 2010-11, Vol.93 (6), p.1897-1904</ispartof><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2010 Oxford University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-1435b527f3797444aa97130c177467de5101bd8751661c39c6a6f6e7c117f8643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23797452$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21313818$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ELLINGSON, David</creatorcontrib><creatorcontrib>POTTS, Brian</creatorcontrib><creatorcontrib>ANDERSON, Phillip</creatorcontrib><creatorcontrib>BURKHARDT, Greg</creatorcontrib><creatorcontrib>ELLEFSON, Wayne</creatorcontrib><creatorcontrib>SULLIVAN, Darryl</creatorcontrib><creatorcontrib>JACOBS, Wesley</creatorcontrib><creatorcontrib>RAGAN, Robert</creatorcontrib><title>Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>An improved method for direct determination of available carbohydrates in low-level products has been developed and validated for a low-carbohydrate soy infant formula. The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0% across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4%, respectively. Overall average recovery was 99.4% (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106% (n = 10). It is expected that the method could be applied to a variety of low-carbohydrate foods and beverages.</description><subject>Analysis</subject><subject>Anion Exchange Resins</subject><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Carbohydrates</subject><subject>Chromatography, Ion Exchange</subject><subject>Dietary Carbohydrates - analysis</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactose</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Indicators and Reagents</subject><subject>Infant</subject><subject>Infant Food - analysis</subject><subject>Low carbohydrate foods</subject><subject>Physiological aspects</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Solutions</subject><subject>Starch - analysis</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU9v1DAQxSMEoqVw5oYsIcQpux7bsZPjalso0iJ6oOdo4jiJqyRebC-w36Ifud7u8k9CPng0-r03o3lZ9hroAmjFl3foUC8rvpALKCv1JDuHSohcVYw9TTWVNOdMwVn2IoQ7SgVIyp5nZww48BLK8-z-s4mDa0nnPImDIZfWGx3JpYnGT3bGaN1MXEdW39GO2IyGrNE3bti3HqMJxM5k437kfzfJjXftTsdAboOde3Jt-yG_MT6NmHDWhqzmg-nVTz3g3CfDwbsJo-s9bof9y-xZh2Mwr07_RXb74err-jrffPn4ab3a5JpXPOYgeNEUTHVcVUoIgVgp4FSDUkKq1hRAoWlLVYCUkCRaouykURpAdaUU_CJ7f_TdevdtZ0KsJxu0GUecjduFuixAiRIUS-TbI9njaGo7dy561Ae6XjGZJlSsgEQt_kOl15rJajebzqb-P4LlUaC9C8Gbrt56O6Hf10DrQ7j1Y7h1KmR9CDcp3pxW3jWTaX_zv9JMwLsTgEHj2Pl0bhv-cI-3Khh_AAMBrRQ</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>ELLINGSON, David</creator><creator>POTTS, Brian</creator><creator>ANDERSON, Phillip</creator><creator>BURKHARDT, Greg</creator><creator>ELLEFSON, Wayne</creator><creator>SULLIVAN, Darryl</creator><creator>JACOBS, Wesley</creator><creator>RAGAN, Robert</creator><general>AOAC International</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography</title><author>ELLINGSON, David ; POTTS, Brian ; ANDERSON, Phillip ; BURKHARDT, Greg ; ELLEFSON, Wayne ; SULLIVAN, Darryl ; JACOBS, Wesley ; RAGAN, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-1435b527f3797444aa97130c177467de5101bd8751661c39c6a6f6e7c117f8643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analysis</topic><topic>Anion Exchange Resins</topic><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Carbohydrates</topic><topic>Chromatography, Ion Exchange</topic><topic>Dietary Carbohydrates - analysis</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. 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The method involves modification of an existing direct determination method to improve specificity, accuracy, detection levels, and run times through a more extensive enzymatic digestion to capture all available (or potentially available) carbohydrates. The digestion hydrolyzes all common sugars, starch, and starch derivatives down to their monosaccharide components, glucose, fructose, and galactose, which are then quantitated by high-performance anion-exchange chromatography with photodiode array detection. Method validation consisted of specificity testing and 10 days of analyzing various spike levels of mixed sugars, maltodextrin, and corn starch. The overall RSD was 4.0% across all sample types, which contained within-day and day-to-day components of 3.6 and 3.4%, respectively. Overall average recovery was 99.4% (n = 10). Average recovery for individual spiked samples ranged from 94.1 to 106% (n = 10). 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| identifier | ISSN: 1060-3271 |
| ispartof | Journal of AOAC International, 2010-11, Vol.93 (6), p.1897-1904 |
| issn | 1060-3271 1944-7922 |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | Analysis Anion Exchange Resins Biological and medical sciences Calibration Carbohydrates Chromatography, Ion Exchange Dietary Carbohydrates - analysis Food industries Fundamental and applied biological sciences. Psychology Galactose High performance liquid chromatography Humans Indicators and Reagents Infant Infant Food - analysis Low carbohydrate foods Physiological aspects Reference Standards Reproducibility of Results Solutions Starch - analysis |
| title | Method for the Direct Determination of Available Carbohydrates in Low-Carbohydrate Products Using High-Performance Anion Exchange Chromatography |
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