First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain
In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protoc...
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| Veröffentlicht in: | Journal of neuroscience methods 2010-12, Vol.194 (1), p.154-157 |
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| container_title | Journal of neuroscience methods |
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| creator | Unsworth, C.P. Graham, E.S. Delivopoulos, E. Dragunow, M. Murray, A.F. |
| description | In this communication, we describe a new method which has enabled the first patterning of human neurons (derived from the human teratocarcinoma cell line (hNT)) on parylene-C/silicon dioxide substrates. We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are each key aspects of this new method. The benefits in patterning human neurons on silicon chip using an accessible cell line and robust patterning technology are of widespread value. Thus, using a combined technology such as this will facilitate the detailed study of the pathological human brain at both the single cell and network level. |
| doi_str_mv | 10.1016/j.jneumeth.2010.09.022 |
| format | Article |
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We reveal the details of the nanofabrication processes, cell differentiation and culturing protocols necessary to successfully pattern hNT neurons which are each key aspects of this new method. The benefits in patterning human neurons on silicon chip using an accessible cell line and robust patterning technology are of widespread value. 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Thus, using a combined technology such as this will facilitate the detailed study of the pathological human brain at both the single cell and network level.</description><subject>Animals</subject><subject>Brain - pathology</subject><subject>Brain Neoplasms - pathology</subject><subject>Cell Differentiation</subject><subject>Cell Line, Tumor</subject><subject>Cell patterning</subject><subject>Cytological Techniques</subject><subject>hNT neuron</subject><subject>Human brain cell</subject><subject>Humans</subject><subject>Lab-On-A-Chip Devices</subject><subject>Microcomputers</subject><subject>Microtechnology</subject><subject>Neurons - pathology</subject><subject>NT2</subject><subject>NTera2 cell line</subject><subject>On chip</subject><subject>Parylene</subject><subject>Parylene-C</subject><subject>Polymers</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Silicon chip</subject><subject>Silicon Dioxide</subject><subject>Teratoma - pathology</subject><subject>Xylenes</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGO0zAQhi0EYsvCK6x845TuOE3jmBOoYgFpBZdF4mY59mTjyrGL7azo6_FkOLTlysma8Tf_7_FPyA2DNQPW3u7Xe4_zhHlc11CaINZQ18_IinW8rlre_XhOVgXcVlBzuCKvUtoDQCOgfUmuahCbTalW5PedjSnTcZ6Up-PXB1pUY_CJHlTOGD0aGnwp4tGhx2p3m6yzurSMDb-sQZrmPuWoMqZ3dBem3nrrH2kRU1pjSrZ3SDU6R531WPqGxtDPxfJssNAZ9eiDC49HOoRI81hk82yONAx_i4KOy7XVylFlZnd5cB-V9a_Ji0G5hG_O5zX5fvfxYfe5uv_26cvuw32lN0LkinNQNQ5GDIbXKIxA0XdNj0apBhpUjWJG87Y1Xcc3W1CaQ8sYB8YGbLBXm2vy9qR7iOHnjCnLyaZlNeUxzEl2W9a0XdtBIdsTqWNIKeIgD9FO5Q8lA7nEJ_fyEp9c4pMgZImvDN6cLeZ-QvNv7JJXAd6fACyLPlmMMmmLXqOxEXWWJtj_efwB9GK1eg</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>Unsworth, C.P.</creator><creator>Graham, E.S.</creator><creator>Delivopoulos, E.</creator><creator>Dragunow, M.</creator><creator>Murray, A.F.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>20101215</creationdate><title>First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain</title><author>Unsworth, C.P. ; 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| identifier | ISSN: 0165-0270 |
| ispartof | Journal of neuroscience methods, 2010-12, Vol.194 (1), p.154-157 |
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| language | eng |
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| subjects | Animals Brain - pathology Brain Neoplasms - pathology Cell Differentiation Cell Line, Tumor Cell patterning Cytological Techniques hNT neuron Human brain cell Humans Lab-On-A-Chip Devices Microcomputers Microtechnology Neurons - pathology NT2 NTera2 cell line On chip Parylene Parylene-C Polymers Rats Rats, Sprague-Dawley Silicon chip Silicon Dioxide Teratoma - pathology Xylenes |
| title | First human hNT neurons patterned on parylene-C/silicon dioxide substrates: Combining an accessible cell line and robust patterning technology for the study of the pathological adult human brain |
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