Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction
Abstract Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae , were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 255...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2010-02, Vol.397 (2), p.399-408 |
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| creator | Eusebio-Cope, Ana Sun, Liying Hillman, Bradley I Suzuki, Nobuhiro |
| description | Abstract Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae , were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557–2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80–90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent. |
| doi_str_mv | 10.1016/j.virol.2009.11.035 |
| format | Article |
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Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80–90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. 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Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80–90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent.</description><subject>Ascomycota - virology</subject><subject>Chestnut blight</subject><subject>Cryphonectria parasitica</subject><subject>dsRNA</subject><subject>Hypovirulence</subject><subject>Hypovirus</subject><subject>Infectious Disease</subject><subject>mutagenesis</subject><subject>Mycovirus</subject><subject>mycoviruses</subject><subject>Open Reading Frames</subject><subject>Rearrangement</subject><subject>Recombination, Genetic</subject><subject>Reoviridae</subject><subject>Reoviridae - genetics</subject><subject>Reoviridae - pathogenicity</subject><subject>Reoviridae - physiology</subject><subject>Reovirus</subject><subject>Sequence Deletion</subject><subject>Symptom expression</subject><subject>viral proteins</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - physiology</subject><subject>Virocontrol</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - physiology</subject><subject>Virus Replication</subject><subject>Virus transmission</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQhyMEokvhCZDAN04JYzuJ4wNIVcU_qYjD0rPl2BPkJbGDnay0z8EL43QLBy49Wba-32g83xTFSwoVBdq-PVRHF8NYMQBZUVoBbx4VOwqyLYHX9HGxA6hZ2XaMXRTPUjpAvgsBT4uLHIGWt92u-P31ZELEkEutiVCyr0sTLFoyx7Cg88QlYl2a0Sfdj0iGEElm9UgizqMzenHBk35diEeDKel4umNwGJxx6BdyxLhkbiRL1D5NLqUtob0lPsQpv6fTNC9hIs7b1WzlnhdPBj0mfHF_Xha3Hz98v_5c3nz79OX66qY0TQdLWWu0EoZ2QFsjiFpQ08AgadMy2WgtGLdCmBblwLGTou6Z5C3r-w617tD0_LJ4c66b__prxbSo3J3BcdQew5pU19C6payWD5KCc5GVyC6T_EyaGFKKOKg5uilPRVFQmzZ1UHfa1KZNUaqytpx6dV9_7Se0_zJ_PWXg9RkYdFD6R3RJ3e4ZUA604xxqyMS7M4F5YkeHUaVt_gati2gWZYN7oIX3_-XN6Pwm7ieeMB3CGn2WoahKTIHab7u1rRZIACFow_8AHATLsg</recordid><startdate>20100220</startdate><enddate>20100220</enddate><creator>Eusebio-Cope, Ana</creator><creator>Sun, Liying</creator><creator>Hillman, Bradley I</creator><creator>Suzuki, Nobuhiro</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20100220</creationdate><title>Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction</title><author>Eusebio-Cope, Ana ; 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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Ascomycota - virology Chestnut blight Cryphonectria parasitica dsRNA Hypovirulence Hypovirus Infectious Disease mutagenesis Mycovirus mycoviruses Open Reading Frames Rearrangement Recombination, Genetic Reoviridae Reoviridae - genetics Reoviridae - pathogenicity Reoviridae - physiology Reovirus Sequence Deletion Symptom expression viral proteins Viral Proteins - genetics Viral Proteins - physiology Virocontrol Virulence Factors - genetics Virulence Factors - physiology Virus Replication Virus transmission |
| title | Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction |
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