Isolation and chemical characterization of delta 5,7,24-cholestatrien-3 beta-ol from pig tissues
Although indirect evidence has implicated Delta(5,7,24)-cholestatrien-3-ol as a possible intermediate in cholesterol biosynthesis, this sterol has not previously been isolated from tissues. Administration of two inhibitors of cholesterol biosynthesis to pigs led to the accumulation of Delta(5,7,24)-...
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| Veröffentlicht in: | Journal of lipid research 1969-01, Vol.10 (1), p.121-127 |
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| container_title | Journal of lipid research |
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| creator | Scallen, T J Dean, W J Loughran, E D Vora, B V |
| description | Although indirect evidence has implicated Delta(5,7,24)-cholestatrien-3-ol as a possible intermediate in cholesterol biosynthesis, this sterol has not previously been isolated from tissues. Administration of two inhibitors of cholesterol biosynthesis to pigs led to the accumulation of Delta(5,7,24)-cholestatrien-3-ol in the tissues, and this sterol was isolated from the lung. Proof of its chemical identity was based upon UV, IR, NMR, circular dichroism, and mass spectra, as well as comparison with synthetic Delta(5,7,24)-cholestatrien-3-ol. A fragment at m/e 143 is particularly prominent in the mass spectrum of Delta(5,7)-sterols, and this fact may prove useful for the detection of this functional group. It is proposed that Delta(5,7,24)-cholestatrien-3-ol may be an intermediate in sterol biosynthesis in both animals and plants. |
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Administration of two inhibitors of cholesterol biosynthesis to pigs led to the accumulation of Delta(5,7,24)-cholestatrien-3-ol in the tissues, and this sterol was isolated from the lung. Proof of its chemical identity was based upon UV, IR, NMR, circular dichroism, and mass spectra, as well as comparison with synthetic Delta(5,7,24)-cholestatrien-3-ol. A fragment at m/e 143 is particularly prominent in the mass spectrum of Delta(5,7)-sterols, and this fact may prove useful for the detection of this functional group. It is proposed that Delta(5,7,24)-cholestatrien-3-ol may be an intermediate in sterol biosynthesis in both animals and plants.</description><identifier>ISSN: 0022-2275</identifier><identifier>PMID: 5764111</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Cholesterol - biosynthesis ; Chromatography, Thin Layer ; Infrared Rays ; Liver - metabolism ; Lung - metabolism ; Magnetic Resonance Spectroscopy ; Molecular Weight ; Spectrophotometry ; Spectrum Analysis ; Sterols - analysis ; Sterols - isolation & purification ; Swine ; Ultraviolet Rays</subject><ispartof>Journal of lipid research, 1969-01, Vol.10 (1), p.121-127</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5764111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scallen, T J</creatorcontrib><creatorcontrib>Dean, W J</creatorcontrib><creatorcontrib>Loughran, E D</creatorcontrib><creatorcontrib>Vora, B V</creatorcontrib><title>Isolation and chemical characterization of delta 5,7,24-cholestatrien-3 beta-ol from pig tissues</title><title>Journal of lipid research</title><addtitle>J Lipid Res</addtitle><description>Although indirect evidence has implicated Delta(5,7,24)-cholestatrien-3-ol as a possible intermediate in cholesterol biosynthesis, this sterol has not previously been isolated from tissues. Administration of two inhibitors of cholesterol biosynthesis to pigs led to the accumulation of Delta(5,7,24)-cholestatrien-3-ol in the tissues, and this sterol was isolated from the lung. Proof of its chemical identity was based upon UV, IR, NMR, circular dichroism, and mass spectra, as well as comparison with synthetic Delta(5,7,24)-cholestatrien-3-ol. A fragment at m/e 143 is particularly prominent in the mass spectrum of Delta(5,7)-sterols, and this fact may prove useful for the detection of this functional group. It is proposed that Delta(5,7,24)-cholestatrien-3-ol may be an intermediate in sterol biosynthesis in both animals and plants.</description><subject>Animals</subject><subject>Cholesterol - biosynthesis</subject><subject>Chromatography, Thin Layer</subject><subject>Infrared Rays</subject><subject>Liver - metabolism</subject><subject>Lung - metabolism</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Molecular Weight</subject><subject>Spectrophotometry</subject><subject>Spectrum Analysis</subject><subject>Sterols - analysis</subject><subject>Sterols - isolation & purification</subject><subject>Swine</subject><subject>Ultraviolet Rays</subject><issn>0022-2275</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1969</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotUMtOwzAQ9AFUSuETkHziVEteP5r4iCoelSr1AufgOBtq5MTBdg_w9URqTzPSPHY1V2TJuRBMiErfkNucvzkHpTawIAtdbRQALMnnLsdgi48jtWNH3REH72yYiU3WFUz-76zGnnYYiqV6Xa2FYu4YA-ZiS_I4MklbLJbFQPsUBzr5L1p8zifMd-S6tyHj_QVX5OPl-X37xvaH1932ac8mEFBYByhAco4GjAQOjtteOu0M3-jOOKXq-WVtK9Age9NqCYLzCnmnZGva2sgVeTz3Tin-zHdLM_jsMAQ7Yjzlpp6DQtXVbHy4GE_tgF0zJT_Y9NtcJpH_QYlabQ</recordid><startdate>196901</startdate><enddate>196901</enddate><creator>Scallen, T J</creator><creator>Dean, W J</creator><creator>Loughran, E D</creator><creator>Vora, B V</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>196901</creationdate><title>Isolation and chemical characterization of delta 5,7,24-cholestatrien-3 beta-ol from pig tissues</title><author>Scallen, T J ; Dean, W J ; Loughran, E D ; Vora, B V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p121t-d1e21300e9193101c0af3c5c9065d9c4485765a71513f9b5312007e0d43b9b893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1969</creationdate><topic>Animals</topic><topic>Cholesterol - biosynthesis</topic><topic>Chromatography, Thin Layer</topic><topic>Infrared Rays</topic><topic>Liver - metabolism</topic><topic>Lung - metabolism</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Molecular Weight</topic><topic>Spectrophotometry</topic><topic>Spectrum Analysis</topic><topic>Sterols - analysis</topic><topic>Sterols - isolation & purification</topic><topic>Swine</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scallen, T J</creatorcontrib><creatorcontrib>Dean, W J</creatorcontrib><creatorcontrib>Loughran, E D</creatorcontrib><creatorcontrib>Vora, B V</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of lipid research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scallen, T J</au><au>Dean, W J</au><au>Loughran, E D</au><au>Vora, B V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and chemical characterization of delta 5,7,24-cholestatrien-3 beta-ol from pig tissues</atitle><jtitle>Journal of lipid research</jtitle><addtitle>J Lipid Res</addtitle><date>1969-01</date><risdate>1969</risdate><volume>10</volume><issue>1</issue><spage>121</spage><epage>127</epage><pages>121-127</pages><issn>0022-2275</issn><abstract>Although indirect evidence has implicated Delta(5,7,24)-cholestatrien-3-ol as a possible intermediate in cholesterol biosynthesis, this sterol has not previously been isolated from tissues. Administration of two inhibitors of cholesterol biosynthesis to pigs led to the accumulation of Delta(5,7,24)-cholestatrien-3-ol in the tissues, and this sterol was isolated from the lung. Proof of its chemical identity was based upon UV, IR, NMR, circular dichroism, and mass spectra, as well as comparison with synthetic Delta(5,7,24)-cholestatrien-3-ol. A fragment at m/e 143 is particularly prominent in the mass spectrum of Delta(5,7)-sterols, and this fact may prove useful for the detection of this functional group. It is proposed that Delta(5,7,24)-cholestatrien-3-ol may be an intermediate in sterol biosynthesis in both animals and plants.</abstract><cop>United States</cop><pmid>5764111</pmid><tpages>7</tpages></addata></record> |
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| source | American Society for Biochemistry and Molecular Biology; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animals Cholesterol - biosynthesis Chromatography, Thin Layer Infrared Rays Liver - metabolism Lung - metabolism Magnetic Resonance Spectroscopy Molecular Weight Spectrophotometry Spectrum Analysis Sterols - analysis Sterols - isolation & purification Swine Ultraviolet Rays |
| title | Isolation and chemical characterization of delta 5,7,24-cholestatrien-3 beta-ol from pig tissues |
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