A micro quantitative determination of total fatty acids in biological samples by esterification with radioactive methanol
By the application of the isotope derivative technique with radioactive methanol, a procedure for measuring esterified and nonesterified fatty acids was devised. The present method is ideally suited to measuring large numbers of biological samples when total fatty acid content is required. The wide...
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| Veröffentlicht in: | Anal. Biochem., 25: 432-9(Oct. 24, 1968) 25: 432-9(Oct. 24, 1968), 1968, Vol.25 (1), p.432-439 |
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| container_title | Anal. Biochem., 25: 432-9(Oct. 24, 1968) |
| container_volume | 25 |
| creator | Fischer, George A. Kabara, Jon J. |
| description | By the application of the isotope derivative technique with radioactive methanol, a procedure for measuring esterified and nonesterified fatty acids was devised.
The present method is ideally suited to measuring large numbers of biological samples when total fatty acid content is required. The wide range of sample size, short time required for measurement, applicability to both free and esterified fatty acids, and possibility of computorized data processing make the present method a valuable supplement to gasliquid chromatography. |
| doi_str_mv | 10.1016/0003-2697(68)90118-8 |
| format | Article |
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The present method is ideally suited to measuring large numbers of biological samples when total fatty acid content is required. The wide range of sample size, short time required for measurement, applicability to both free and esterified fatty acids, and possibility of computorized data processing make the present method a valuable supplement to gasliquid chromatography.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(68)90118-8</identifier><identifier>PMID: 5704759</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>BIOLOGICAL MATERIALS ; BIOLOGICAL MATERIALS/analysis for fatty acids by esterification with tritium-labeled methanol ; BIOLOGY ; CHEMICAL REACTIONS ; Cholesterol - analysis ; Chromatography, Gas ; DETERMINATION ; ESTERIFICATION ; FATTY ACIDS ; Fatty Acids - analysis ; Fatty Acids, Nonesterified - analysis ; FATTY ACIDS/determination in biological samples by esterification with tritium-labeled methanol ; LABELLED COMPOUNDS ; Mathematics ; METHANOL ; METHANOL/use of tritium-labeled, in determination of fatty acids in biological samples ; Methods ; Methylation ; Microchemistry ; N20120 -Chemistry-Analytical & Separations Chemistry- Isotopic Procedures ; Oleic Acids - analysis ; ORGANIC ACIDS ; Palmitic Acids - analysis ; Stearic Acids - analysis ; TRITIUM ; TRITIUM/incorporation in methanol, use in determination of fatty acids in biological samples</subject><ispartof>Anal. Biochem., 25: 432-9(Oct. 24, 1968), 1968, Vol.25 (1), p.432-439</ispartof><rights>1968</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c299t-f2a0a9fbed617b4e70cd5b142d6d23e7dab8ae72ad10068d2356f40c037582043</citedby><cites>FETCH-LOGICAL-c299t-f2a0a9fbed617b4e70cd5b142d6d23e7dab8ae72ad10068d2356f40c037582043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0003269768901188$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,4010,27902,27903,27904,65308</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5704759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4831085$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Fischer, George A.</creatorcontrib><creatorcontrib>Kabara, Jon J.</creatorcontrib><creatorcontrib>Univ. of Detroit</creatorcontrib><title>A micro quantitative determination of total fatty acids in biological samples by esterification with radioactive methanol</title><title>Anal. Biochem., 25: 432-9(Oct. 24, 1968)</title><addtitle>Anal Biochem</addtitle><description>By the application of the isotope derivative technique with radioactive methanol, a procedure for measuring esterified and nonesterified fatty acids was devised.
The present method is ideally suited to measuring large numbers of biological samples when total fatty acid content is required. The wide range of sample size, short time required for measurement, applicability to both free and esterified fatty acids, and possibility of computorized data processing make the present method a valuable supplement to gasliquid chromatography.</description><subject>BIOLOGICAL MATERIALS</subject><subject>BIOLOGICAL MATERIALS/analysis for fatty acids by esterification with tritium-labeled methanol</subject><subject>BIOLOGY</subject><subject>CHEMICAL REACTIONS</subject><subject>Cholesterol - analysis</subject><subject>Chromatography, Gas</subject><subject>DETERMINATION</subject><subject>ESTERIFICATION</subject><subject>FATTY ACIDS</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids, Nonesterified - analysis</subject><subject>FATTY ACIDS/determination in biological samples by esterification with tritium-labeled methanol</subject><subject>LABELLED COMPOUNDS</subject><subject>Mathematics</subject><subject>METHANOL</subject><subject>METHANOL/use of tritium-labeled, in determination of fatty acids in biological samples</subject><subject>Methods</subject><subject>Methylation</subject><subject>Microchemistry</subject><subject>N20120 -Chemistry-Analytical & Separations Chemistry- Isotopic Procedures</subject><subject>Oleic Acids - analysis</subject><subject>ORGANIC ACIDS</subject><subject>Palmitic Acids - analysis</subject><subject>Stearic Acids - analysis</subject><subject>TRITIUM</subject><subject>TRITIUM/incorporation in methanol, use in determination of fatty acids in biological samples</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uctu1DAUtRCoTAt_AJLFApVF4DqJH9lUqipeUiU2sLYc-4YxSuyp7Smav8eZGXXJyvI9D1-fQ8gbBh8ZMPEJALqmFYO8FurDAIypRj0jGwaDaKCD4TnZPFFeksuc_0Al9VxckAsuoZd82JDDLV28TZE-7E0ovpjiH5E6LJgWH-otBhonWmIxM51MKQdqrHeZ-kBHH-f429uKZLPsZsx0PFDMVeunOj6K__qypck4H409ei9YtibE-RV5MZk54-vzeUV-ffn88-5bc__j6_e72_vGtsNQmqk1YIZpRCeYHHuUYB0fWd864doOpTOjMihb4xiAUHXGxdSDhU5y1ULfXZF3J9-Yi9fZ-oJ2a2MIaIvuVcdA8Up6fyLtUnzY1y_oxWeL82wCxn3WigOXNchK7E_EmlnOCSe9S34x6aAZ6LUWvWau18y1UPpYi1ZV9vbsvx8XdE-icw8VvznhWJN49JjWRTFYdD6te7ro___APzJ_nkI</recordid><startdate>1968</startdate><enddate>1968</enddate><creator>Fischer, George A.</creator><creator>Kabara, Jon J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>1968</creationdate><title>A micro quantitative determination of total fatty acids in biological samples by esterification with radioactive methanol</title><author>Fischer, George A. ; Kabara, Jon J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c299t-f2a0a9fbed617b4e70cd5b142d6d23e7dab8ae72ad10068d2356f40c037582043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>BIOLOGICAL MATERIALS</topic><topic>BIOLOGICAL MATERIALS/analysis for fatty acids by esterification with tritium-labeled methanol</topic><topic>BIOLOGY</topic><topic>CHEMICAL REACTIONS</topic><topic>Cholesterol - analysis</topic><topic>Chromatography, Gas</topic><topic>DETERMINATION</topic><topic>ESTERIFICATION</topic><topic>FATTY ACIDS</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids, Nonesterified - analysis</topic><topic>FATTY ACIDS/determination in biological samples by esterification with tritium-labeled methanol</topic><topic>LABELLED COMPOUNDS</topic><topic>Mathematics</topic><topic>METHANOL</topic><topic>METHANOL/use of tritium-labeled, in determination of fatty acids in biological samples</topic><topic>Methods</topic><topic>Methylation</topic><topic>Microchemistry</topic><topic>N20120 -Chemistry-Analytical & Separations Chemistry- Isotopic Procedures</topic><topic>Oleic Acids - analysis</topic><topic>ORGANIC ACIDS</topic><topic>Palmitic Acids - analysis</topic><topic>Stearic Acids - analysis</topic><topic>TRITIUM</topic><topic>TRITIUM/incorporation in methanol, use in determination of fatty acids in biological samples</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fischer, George A.</creatorcontrib><creatorcontrib>Kabara, Jon J.</creatorcontrib><creatorcontrib>Univ. of Detroit</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Anal. Biochem., 25: 432-9(Oct. 24, 1968)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fischer, George A.</au><au>Kabara, Jon J.</au><aucorp>Univ. of Detroit</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A micro quantitative determination of total fatty acids in biological samples by esterification with radioactive methanol</atitle><jtitle>Anal. Biochem., 25: 432-9(Oct. 24, 1968)</jtitle><addtitle>Anal Biochem</addtitle><date>1968</date><risdate>1968</risdate><volume>25</volume><issue>1</issue><spage>432</spage><epage>439</epage><pages>432-439</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>By the application of the isotope derivative technique with radioactive methanol, a procedure for measuring esterified and nonesterified fatty acids was devised.
The present method is ideally suited to measuring large numbers of biological samples when total fatty acid content is required. The wide range of sample size, short time required for measurement, applicability to both free and esterified fatty acids, and possibility of computorized data processing make the present method a valuable supplement to gasliquid chromatography.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5704759</pmid><doi>10.1016/0003-2697(68)90118-8</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Anal. Biochem., 25: 432-9(Oct. 24, 1968), 1968, Vol.25 (1), p.432-439 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_85057269 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | BIOLOGICAL MATERIALS BIOLOGICAL MATERIALS/analysis for fatty acids by esterification with tritium-labeled methanol BIOLOGY CHEMICAL REACTIONS Cholesterol - analysis Chromatography, Gas DETERMINATION ESTERIFICATION FATTY ACIDS Fatty Acids - analysis Fatty Acids, Nonesterified - analysis FATTY ACIDS/determination in biological samples by esterification with tritium-labeled methanol LABELLED COMPOUNDS Mathematics METHANOL METHANOL/use of tritium-labeled, in determination of fatty acids in biological samples Methods Methylation Microchemistry N20120 -Chemistry-Analytical & Separations Chemistry- Isotopic Procedures Oleic Acids - analysis ORGANIC ACIDS Palmitic Acids - analysis Stearic Acids - analysis TRITIUM TRITIUM/incorporation in methanol, use in determination of fatty acids in biological samples |
| title | A micro quantitative determination of total fatty acids in biological samples by esterification with radioactive methanol |
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