The glucose catabolism of the genus Brucella: I. Evaluation of pathways
The glucose catobolism of representative strains of the genus Brucella has been examined using the radiorespirometric technique. The radiorespirometric data fit two model systems: (1) operation of the hexosemonophosphate (HMP) pathway in conjunction with the tricarboxylic acid (TCA) cycle and (2) co...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1968-01, Vol.127 (1), p.263-273 |
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| creator | Robertson, D.C. McCullough, W.G. |
| description | The glucose catobolism of representative strains of the genus
Brucella has been examined using the radiorespirometric technique. The radiorespirometric data fit two model systems: (1) operation of the hexosemonophosphate (HMP) pathway in conjunction with the tricarboxylic acid (TCA) cycle and (2) concurrent operation of the HMP and Entner-Doudoroff (ED) pathways in conjunction with the TCA cycle. Only quantitative differences were observed among the species or between attenuated and virulent strains of
Brucella abortus.
The distribution and incorporation of
d-glucose-
14C into pyruvate isolated from culture supernatants of arsenite-poisoned cells and alanine isolated from cell hydrolysates suggested the absence of C
3—C
3 cleavage as would be expected from either the Embden-Meyerhof-Parnas (EMP) pathway or the ED pathway. Trapping experiments with large amounts of “cold” acetate eliminated the heterolactic fermentation mechanism. |
| doi_str_mv | 10.1016/0003-9861(68)90225-7 |
| format | Article |
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Brucella has been examined using the radiorespirometric technique. The radiorespirometric data fit two model systems: (1) operation of the hexosemonophosphate (HMP) pathway in conjunction with the tricarboxylic acid (TCA) cycle and (2) concurrent operation of the HMP and Entner-Doudoroff (ED) pathways in conjunction with the TCA cycle. Only quantitative differences were observed among the species or between attenuated and virulent strains of
Brucella abortus.
The distribution and incorporation of
d-glucose-
14C into pyruvate isolated from culture supernatants of arsenite-poisoned cells and alanine isolated from cell hydrolysates suggested the absence of C
3—C
3 cleavage as would be expected from either the Embden-Meyerhof-Parnas (EMP) pathway or the ED pathway. Trapping experiments with large amounts of “cold” acetate eliminated the heterolactic fermentation mechanism.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(68)90225-7</identifier><identifier>PMID: 4972340</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acetates - biosynthesis ; Alanine - biosynthesis ; Arsenic - pharmacology ; Brucella - metabolism ; Brucella abortus - metabolism ; Carbon Dioxide - metabolism ; Carbon Isotopes ; Citric Acid Cycle ; Depression, Chemical ; Glucose - metabolism ; Hexosephosphates - metabolism ; Oxidation-Reduction ; Pyruvates - biosynthesis ; Species Specificity ; Time Factors ; Virulence</subject><ispartof>Archives of biochemistry and biophysics, 1968-01, Vol.127 (1), p.263-273</ispartof><rights>1968</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(68)90225-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4972340$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Robertson, D.C.</creatorcontrib><creatorcontrib>McCullough, W.G.</creatorcontrib><title>The glucose catabolism of the genus Brucella: I. Evaluation of pathways</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>The glucose catobolism of representative strains of the genus
Brucella has been examined using the radiorespirometric technique. The radiorespirometric data fit two model systems: (1) operation of the hexosemonophosphate (HMP) pathway in conjunction with the tricarboxylic acid (TCA) cycle and (2) concurrent operation of the HMP and Entner-Doudoroff (ED) pathways in conjunction with the TCA cycle. Only quantitative differences were observed among the species or between attenuated and virulent strains of
Brucella abortus.
The distribution and incorporation of
d-glucose-
14C into pyruvate isolated from culture supernatants of arsenite-poisoned cells and alanine isolated from cell hydrolysates suggested the absence of C
3—C
3 cleavage as would be expected from either the Embden-Meyerhof-Parnas (EMP) pathway or the ED pathway. Trapping experiments with large amounts of “cold” acetate eliminated the heterolactic fermentation mechanism.</description><subject>Acetates - biosynthesis</subject><subject>Alanine - biosynthesis</subject><subject>Arsenic - pharmacology</subject><subject>Brucella - metabolism</subject><subject>Brucella abortus - metabolism</subject><subject>Carbon Dioxide - metabolism</subject><subject>Carbon Isotopes</subject><subject>Citric Acid Cycle</subject><subject>Depression, Chemical</subject><subject>Glucose - metabolism</subject><subject>Hexosephosphates - metabolism</subject><subject>Oxidation-Reduction</subject><subject>Pyruvates - biosynthesis</subject><subject>Species Specificity</subject><subject>Time Factors</subject><subject>Virulence</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Lw0AURQdRaq3-A4WsRBepb2YyH3EhaKm1UHBT18Nk8mJHkqZmkkr_vY0trt7iHi73HUKuKYwpUPkAADxOtaR3Ut-nwJiI1QkZUkhlDFwnp2T4j5yTixC-AChNJBuQQZIqxhMYktlyhdFn2bk6YORsa7O69KGK6iJq-wTXXYhems5hWdrHaD6Opltbdrb19bqHNrZd_dhduCRnhS0DXh3viHy8TpeTt3jxPptPnhcxMknbWOoUAJUWeSa4UI5RkAIsUxyRFQVT1GrFrcwQqJBCFVzlTuZCJ5jJNAc-IreH3k1Tf3cYWlP58DdujXUXjBYAIpU9eHMEu6zC3GwaX9lmZ46f7_OnQ477tVuPjQnO49ph7ht0rclrbyiY3rTpNZpeo5Ha_Jk2iv8C_OltVw</recordid><startdate>19680101</startdate><enddate>19680101</enddate><creator>Robertson, D.C.</creator><creator>McCullough, W.G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19680101</creationdate><title>The glucose catabolism of the genus Brucella: I. Evaluation of pathways</title><author>Robertson, D.C. ; McCullough, W.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e261t-68900e785db5357c210650a273ee2ff271a873a6be015657f37dc6d584eb69d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Acetates - biosynthesis</topic><topic>Alanine - biosynthesis</topic><topic>Arsenic - pharmacology</topic><topic>Brucella - metabolism</topic><topic>Brucella abortus - metabolism</topic><topic>Carbon Dioxide - metabolism</topic><topic>Carbon Isotopes</topic><topic>Citric Acid Cycle</topic><topic>Depression, Chemical</topic><topic>Glucose - metabolism</topic><topic>Hexosephosphates - metabolism</topic><topic>Oxidation-Reduction</topic><topic>Pyruvates - biosynthesis</topic><topic>Species Specificity</topic><topic>Time Factors</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Robertson, D.C.</creatorcontrib><creatorcontrib>McCullough, W.G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Robertson, D.C.</au><au>McCullough, W.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The glucose catabolism of the genus Brucella: I. Evaluation of pathways</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1968-01-01</date><risdate>1968</risdate><volume>127</volume><issue>1</issue><spage>263</spage><epage>273</epage><pages>263-273</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>The glucose catobolism of representative strains of the genus
Brucella has been examined using the radiorespirometric technique. The radiorespirometric data fit two model systems: (1) operation of the hexosemonophosphate (HMP) pathway in conjunction with the tricarboxylic acid (TCA) cycle and (2) concurrent operation of the HMP and Entner-Doudoroff (ED) pathways in conjunction with the TCA cycle. Only quantitative differences were observed among the species or between attenuated and virulent strains of
Brucella abortus.
The distribution and incorporation of
d-glucose-
14C into pyruvate isolated from culture supernatants of arsenite-poisoned cells and alanine isolated from cell hydrolysates suggested the absence of C
3—C
3 cleavage as would be expected from either the Embden-Meyerhof-Parnas (EMP) pathway or the ED pathway. Trapping experiments with large amounts of “cold” acetate eliminated the heterolactic fermentation mechanism.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>4972340</pmid><doi>10.1016/0003-9861(68)90225-7</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1968-01, Vol.127 (1), p.263-273 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_85005960 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetates - biosynthesis Alanine - biosynthesis Arsenic - pharmacology Brucella - metabolism Brucella abortus - metabolism Carbon Dioxide - metabolism Carbon Isotopes Citric Acid Cycle Depression, Chemical Glucose - metabolism Hexosephosphates - metabolism Oxidation-Reduction Pyruvates - biosynthesis Species Specificity Time Factors Virulence |
| title | The glucose catabolism of the genus Brucella: I. Evaluation of pathways |
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