Swelling of Human Red Blood Cells, Preserved with Progesterone, as Determined by Spectrophotometric Measurements
Summary The swelling rate of human red blood cells has been studied by spectrophotometric measurements of the absorbance of erythrocytes dispersed in water or sucrose solutions buffered with sodium phosphate buffer, pH 7.4. The rate of swelling of red cells, prepared from blood stored at 2° in acid-...
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| Veröffentlicht in: | Experimental biology and medicine (Maywood, N.J.) N.J.), 1968-10, Vol.129 (1), p.106-110 |
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| container_title | Experimental biology and medicine (Maywood, N.J.) |
| container_volume | 129 |
| creator | DeVenuto, Frank |
| description | Summary
The swelling rate of human red blood cells has been studied by spectrophotometric measurements of the absorbance of erythrocytes dispersed in water or sucrose solutions buffered with sodium phosphate buffer, pH 7.4. The rate of swelling of red cells, prepared from blood stored at 2° in acid-citrate-dextrose solution, increases with increased time of storage. The addition of physiological amounts of progesterone to red blood cells stored in the cold under sterile conditions greatly depresses the increase of the swelling rate; red blood cells preserved for 40 days with addition of progesterone manifest the same rate of swelling as that of red blood cells stored for 10 days without progesterone. |
| doi_str_mv | 10.3181/00379727-129-33262 |
| format | Article |
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The swelling rate of human red blood cells has been studied by spectrophotometric measurements of the absorbance of erythrocytes dispersed in water or sucrose solutions buffered with sodium phosphate buffer, pH 7.4. The rate of swelling of red cells, prepared from blood stored at 2° in acid-citrate-dextrose solution, increases with increased time of storage. The addition of physiological amounts of progesterone to red blood cells stored in the cold under sterile conditions greatly depresses the increase of the swelling rate; red blood cells preserved for 40 days with addition of progesterone manifest the same rate of swelling as that of red blood cells stored for 10 days without progesterone.</description><identifier>ISSN: 0037-9727</identifier><identifier>ISSN: 1535-3702</identifier><identifier>EISSN: 1535-3699</identifier><identifier>DOI: 10.3181/00379727-129-33262</identifier><identifier>PMID: 5686494</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Adolescent ; Adult ; Blood Preservation ; Buffers ; Erythrocytes - drug effects ; Humans ; Male ; Methods ; Phosphates - pharmacology ; Progesterone - pharmacology ; Sodium - pharmacology ; Spectrophotometry ; Sucrose - pharmacology ; Time Factors</subject><ispartof>Experimental biology and medicine (Maywood, N.J.), 1968-10, Vol.129 (1), p.106-110</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c338t-4d8dcaea201db99e13e3fc91189ad1fc2ec5d02af5559756ae29702850fcd1993</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5686494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DeVenuto, Frank</creatorcontrib><title>Swelling of Human Red Blood Cells, Preserved with Progesterone, as Determined by Spectrophotometric Measurements</title><title>Experimental biology and medicine (Maywood, N.J.)</title><addtitle>Proc Soc Exp Biol Med</addtitle><description>Summary
The swelling rate of human red blood cells has been studied by spectrophotometric measurements of the absorbance of erythrocytes dispersed in water or sucrose solutions buffered with sodium phosphate buffer, pH 7.4. The rate of swelling of red cells, prepared from blood stored at 2° in acid-citrate-dextrose solution, increases with increased time of storage. The addition of physiological amounts of progesterone to red blood cells stored in the cold under sterile conditions greatly depresses the increase of the swelling rate; red blood cells preserved for 40 days with addition of progesterone manifest the same rate of swelling as that of red blood cells stored for 10 days without progesterone.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Blood Preservation</subject><subject>Buffers</subject><subject>Erythrocytes - drug effects</subject><subject>Humans</subject><subject>Male</subject><subject>Methods</subject><subject>Phosphates - pharmacology</subject><subject>Progesterone - pharmacology</subject><subject>Sodium - pharmacology</subject><subject>Spectrophotometry</subject><subject>Sucrose - pharmacology</subject><subject>Time Factors</subject><issn>0037-9727</issn><issn>1535-3702</issn><issn>1535-3699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1968</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kElPwzAQhS0EKmX5A0hIPnFqwEucxEcoq1QEYjlbrj1pUzVxsBNQ_z0uLRw5jWbem6eZD6ETSs45LegFITyXOcsTymTCOcvYDhpSwUXCMyl30XBtSNaOfXQQwoIQKnKWDdBAZEWWynSI2tcvWC6rZoZdie_7Wjf4BSy-Wjpn8ThKYYSfPQTwn3H8VXXz2LoZhA68a2CEdcDXEJu6aqJhusKvLZjOu3buOldD5yuDH0GH3kMNTReO0F6plwGOt_UQvd_evI3vk8nT3cP4cpIYzosuSW1hjQbNCLVTKYFy4KWRlBZSW1oaBkZYwnQphJC5yDQwmRNWCFIaS6Xkh-hsk9t699HHe1VdBRMf0g24PqgilUUuKIlGtjEa70LwUKrWV7X2K0WJWmNWv5hVxKx-MMel0216P63B_q1suUb9YqMHPQO1cL1v4rP_JX4DVimHXQ</recordid><startdate>196810</startdate><enddate>196810</enddate><creator>DeVenuto, Frank</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196810</creationdate><title>Swelling of Human Red Blood Cells, Preserved with Progesterone, as Determined by Spectrophotometric Measurements</title><author>DeVenuto, Frank</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c338t-4d8dcaea201db99e13e3fc91189ad1fc2ec5d02af5559756ae29702850fcd1993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1968</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Blood Preservation</topic><topic>Buffers</topic><topic>Erythrocytes - drug effects</topic><topic>Humans</topic><topic>Male</topic><topic>Methods</topic><topic>Phosphates - pharmacology</topic><topic>Progesterone - pharmacology</topic><topic>Sodium - pharmacology</topic><topic>Spectrophotometry</topic><topic>Sucrose - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeVenuto, Frank</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeVenuto, Frank</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Swelling of Human Red Blood Cells, Preserved with Progesterone, as Determined by Spectrophotometric Measurements</atitle><jtitle>Experimental biology and medicine (Maywood, N.J.)</jtitle><addtitle>Proc Soc Exp Biol Med</addtitle><date>1968-10</date><risdate>1968</risdate><volume>129</volume><issue>1</issue><spage>106</spage><epage>110</epage><pages>106-110</pages><issn>0037-9727</issn><issn>1535-3702</issn><eissn>1535-3699</eissn><abstract>Summary
The swelling rate of human red blood cells has been studied by spectrophotometric measurements of the absorbance of erythrocytes dispersed in water or sucrose solutions buffered with sodium phosphate buffer, pH 7.4. The rate of swelling of red cells, prepared from blood stored at 2° in acid-citrate-dextrose solution, increases with increased time of storage. The addition of physiological amounts of progesterone to red blood cells stored in the cold under sterile conditions greatly depresses the increase of the swelling rate; red blood cells preserved for 40 days with addition of progesterone manifest the same rate of swelling as that of red blood cells stored for 10 days without progesterone.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>5686494</pmid><doi>10.3181/00379727-129-33262</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0037-9727 |
| ispartof | Experimental biology and medicine (Maywood, N.J.), 1968-10, Vol.129 (1), p.106-110 |
| issn | 0037-9727 1535-3702 1535-3699 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Adolescent Adult Blood Preservation Buffers Erythrocytes - drug effects Humans Male Methods Phosphates - pharmacology Progesterone - pharmacology Sodium - pharmacology Spectrophotometry Sucrose - pharmacology Time Factors |
| title | Swelling of Human Red Blood Cells, Preserved with Progesterone, as Determined by Spectrophotometric Measurements |
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