Double isotope autoradiography
A double emulsion autoradiographic technique is described which provides a high resolution method of detecting 14C and 3H simultaneously in a single tissue section. Two emulsions are used which differ in their sensitivity to beta radiation. One emulsion is applied to the top of the section, the othe...
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| Veröffentlicht in: | Experimental cell research 1965-09, Vol.39 (2), p.318-328 |
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| Format: | Artikel |
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| container_end_page | 328 |
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| container_issue | 2 |
| container_start_page | 318 |
| container_title | Experimental cell research |
| container_volume | 39 |
| creator | Trelstad, R.L. |
| description | A double emulsion autoradiographic technique is described which provides a high resolution method of detecting
14C and
3H simultaneously in a single tissue section. Two emulsions are used which differ in their sensitivity to beta radiation. One emulsion is applied to the top of the section, the other to the bottom. The top emulsion is sensitive to
3H beta particles and insensitive to
14C. Specificity for
14C is accomplished by using a celloidin film which separates the second
14C-sensitive emulsion from close contact with the section. Both emulsions are exposed and processed together. The final autoradiograph is viewed on a single slide. The
14C emulsion can be seen by focusing on the lower side of the section and the
3H emulsion by focusing on the upper side. |
| doi_str_mv | 10.1016/0014-4827(65)90037-6 |
| format | Article |
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14C and
3H simultaneously in a single tissue section. Two emulsions are used which differ in their sensitivity to beta radiation. One emulsion is applied to the top of the section, the other to the bottom. The top emulsion is sensitive to
3H beta particles and insensitive to
14C. Specificity for
14C is accomplished by using a celloidin film which separates the second
14C-sensitive emulsion from close contact with the section. Both emulsions are exposed and processed together. The final autoradiograph is viewed on a single slide. The
14C emulsion can be seen by focusing on the lower side of the section and the
3H emulsion by focusing on the upper side.</description><identifier>ISSN: 0014-4827</identifier><identifier>EISSN: 1090-2422</identifier><identifier>DOI: 10.1016/0014-4827(65)90037-6</identifier><identifier>PMID: 5838684</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Autoradiography ; Carbon Isotopes ; Chick Embryo ; DNA - biosynthesis ; In Vitro Techniques ; RNA - biosynthesis ; Thymidine - metabolism ; Tritium ; Uridine - metabolism</subject><ispartof>Experimental cell research, 1965-09, Vol.39 (2), p.318-328</ispartof><rights>1965</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-f8a8226876bb26e461cf8505d0e3aef956731abc56f82ea6fa04449aef1f0e973</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014482765900376$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5838684$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trelstad, R.L.</creatorcontrib><title>Double isotope autoradiography</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>A double emulsion autoradiographic technique is described which provides a high resolution method of detecting
14C and
3H simultaneously in a single tissue section. Two emulsions are used which differ in their sensitivity to beta radiation. One emulsion is applied to the top of the section, the other to the bottom. The top emulsion is sensitive to
3H beta particles and insensitive to
14C. Specificity for
14C is accomplished by using a celloidin film which separates the second
14C-sensitive emulsion from close contact with the section. Both emulsions are exposed and processed together. The final autoradiograph is viewed on a single slide. The
14C emulsion can be seen by focusing on the lower side of the section and the
3H emulsion by focusing on the upper side.</description><subject>Animals</subject><subject>Autoradiography</subject><subject>Carbon Isotopes</subject><subject>Chick Embryo</subject><subject>DNA - biosynthesis</subject><subject>In Vitro Techniques</subject><subject>RNA - biosynthesis</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Uridine - metabolism</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1965</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi0EKqXwBoA6IRgCx45vWZBQuUqVWGC2HOcYgtI62AlS356UVh2ZzvBfjr6fkFMK1xSovAGgPOOaqUsprgqAXGVyj4wpFJAxztg-Ge8sh-QopS8A0JrKERkJnWup-Zic34e-bHBap9CFFqe270K0VR0-om0_V8fkwNsm4cn2Tsj748Pb7Dmbvz69zO7mmcuF6jKvrWZMaiXLkknkkjqvBYgKMLfoCyFVTm3phPSaoZXeAue8GCTqAQuVT8jFpreN4bvH1JlFnRw2jV1i6JPRvFCKqXww8o3RxZBSRG_aWC9sXBkKZj2LWTObNbORwvzNYuQQO9v29-UCq11ou8Og3250HCB_aowmuRqXDqs6outMFer_H_wCbXZwJg</recordid><startdate>196509</startdate><enddate>196509</enddate><creator>Trelstad, R.L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>196509</creationdate><title>Double isotope autoradiography</title><author>Trelstad, R.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-f8a8226876bb26e461cf8505d0e3aef956731abc56f82ea6fa04449aef1f0e973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1965</creationdate><topic>Animals</topic><topic>Autoradiography</topic><topic>Carbon Isotopes</topic><topic>Chick Embryo</topic><topic>DNA - biosynthesis</topic><topic>In Vitro Techniques</topic><topic>RNA - biosynthesis</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Uridine - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trelstad, R.L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trelstad, R.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Double isotope autoradiography</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1965-09</date><risdate>1965</risdate><volume>39</volume><issue>2</issue><spage>318</spage><epage>328</epage><pages>318-328</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><abstract>A double emulsion autoradiographic technique is described which provides a high resolution method of detecting
14C and
3H simultaneously in a single tissue section. Two emulsions are used which differ in their sensitivity to beta radiation. One emulsion is applied to the top of the section, the other to the bottom. The top emulsion is sensitive to
3H beta particles and insensitive to
14C. Specificity for
14C is accomplished by using a celloidin film which separates the second
14C-sensitive emulsion from close contact with the section. Both emulsions are exposed and processed together. The final autoradiograph is viewed on a single slide. The
14C emulsion can be seen by focusing on the lower side of the section and the
3H emulsion by focusing on the upper side.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>5838684</pmid><doi>10.1016/0014-4827(65)90037-6</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4827 |
| ispartof | Experimental cell research, 1965-09, Vol.39 (2), p.318-328 |
| issn | 0014-4827 1090-2422 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_84977273 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Autoradiography Carbon Isotopes Chick Embryo DNA - biosynthesis In Vitro Techniques RNA - biosynthesis Thymidine - metabolism Tritium Uridine - metabolism |
| title | Double isotope autoradiography |
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