Insensitivity to Valine of Streptomycin-Dependent Escherichia coli K12
A streptomycin dependent mutant was obtained from wild-type (valine-sensitive) Escherichia coli K12. This mutant ( E. coli DK 12) grew exponentially in minimal medium containing valine (plus dihydrostreptomycin). The inhibition by valine of the acetohydroxy acid synthetase from both dependent and pa...
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| Veröffentlicht in: | Molecular pharmacology 1965-07, Vol.1 (1), p.109-112 |
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| container_title | Molecular pharmacology |
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| creator | Polglase, W J |
| description | A streptomycin dependent mutant was
obtained from wild-type (valine-sensitive)
Escherichia coli K12. This mutant ( E. coli
DK 12) grew exponentially in minimal
medium containing valine (plus dihydrostreptomycin). The inhibition by valine of
the acetohydroxy acid synthetase from
both dependent and parent cells was quantitatively similar. However, the activity of
the acetohydroxy acid synthetase of DK 12
cells was higher than that of the parent
cells. It was concluded that the insensitivity to valine of E. coli DK 12 was the
result of derepression of (acetohydroxy)
acid synthetase in this mutant, the role of
the antibiotic in dependent cells being that
of a "derepressor." |
| format | Article |
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obtained from wild-type (valine-sensitive)
Escherichia coli K12. This mutant ( E. coli
DK 12) grew exponentially in minimal
medium containing valine (plus dihydrostreptomycin). The inhibition by valine of
the acetohydroxy acid synthetase from
both dependent and parent cells was quantitatively similar. However, the activity of
the acetohydroxy acid synthetase of DK 12
cells was higher than that of the parent
cells. It was concluded that the insensitivity to valine of E. coli DK 12 was the
result of derepression of (acetohydroxy)
acid synthetase in this mutant, the role of
the antibiotic in dependent cells being that
of a "derepressor."</description><identifier>ISSN: 0026-895X</identifier><identifier>EISSN: 1521-0111</identifier><identifier>PMID: 5319648</identifier><language>eng</language><publisher>United States: American Society for Pharmacology and Experimental Therapeutics</publisher><subject>Culture Media ; Enzyme Repression ; Escherichia coli - drug effects ; Escherichia coli - metabolism ; In Vitro Techniques ; Ligases ; Molecular Biology ; Mutation ; Streptomycin - pharmacology ; Valine - pharmacology</subject><ispartof>Molecular pharmacology, 1965-07, Vol.1 (1), p.109-112</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5319648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Polglase, W J</creatorcontrib><title>Insensitivity to Valine of Streptomycin-Dependent Escherichia coli K12</title><title>Molecular pharmacology</title><addtitle>Mol Pharmacol</addtitle><description>A streptomycin dependent mutant was
obtained from wild-type (valine-sensitive)
Escherichia coli K12. This mutant ( E. coli
DK 12) grew exponentially in minimal
medium containing valine (plus dihydrostreptomycin). The inhibition by valine of
the acetohydroxy acid synthetase from
both dependent and parent cells was quantitatively similar. However, the activity of
the acetohydroxy acid synthetase of DK 12
cells was higher than that of the parent
cells. It was concluded that the insensitivity to valine of E. coli DK 12 was the
result of derepression of (acetohydroxy)
acid synthetase in this mutant, the role of
the antibiotic in dependent cells being that
of a "derepressor."</description><subject>Culture Media</subject><subject>Enzyme Repression</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - metabolism</subject><subject>In Vitro Techniques</subject><subject>Ligases</subject><subject>Molecular Biology</subject><subject>Mutation</subject><subject>Streptomycin - pharmacology</subject><subject>Valine - pharmacology</subject><issn>0026-895X</issn><issn>1521-0111</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1965</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNotj89LwzAcxYMoc07_BCEH8VZImqZNjjI3HQ48-ANvJcu-WSNtUpNU6X_vxsY7vMP78HjvDE0pz2lGKKXnaEpIXmZC8q9LdBXjNyG04IJM0IQzKstCTNFy5SK4aJP9tWnEyeNP1VoH2Bv8lgL0yXejti57hB7cFlzCi6gbCFY3VmHtW4tfaH6NLoxqI9ycfIY-lov3-XO2fn1azR_WWZOzKu2nGMNKSTQVXDEoSCEMFXSjNluuK5OXvCDKEMkpAQEm11xrpaUpSl0pAhWboftjbx_8zwAx1Z2NGtpWOfBDrEUhuaTsAN6ewGHTwbbug-1UGOvT8X1-d8wbu2v-bIC6b1To1P6Q3401PYhI9g95v2JA</recordid><startdate>196507</startdate><enddate>196507</enddate><creator>Polglase, W J</creator><general>American Society for Pharmacology and Experimental Therapeutics</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>196507</creationdate><title>Insensitivity to Valine of Streptomycin-Dependent Escherichia coli K12</title><author>Polglase, W J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h237t-89ff3690c185a3e4048f181babd5c7f26540af09510e8ef2c5ccac9f46c7a0e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1965</creationdate><topic>Culture Media</topic><topic>Enzyme Repression</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - metabolism</topic><topic>In Vitro Techniques</topic><topic>Ligases</topic><topic>Molecular Biology</topic><topic>Mutation</topic><topic>Streptomycin - pharmacology</topic><topic>Valine - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Polglase, W J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Polglase, W J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insensitivity to Valine of Streptomycin-Dependent Escherichia coli K12</atitle><jtitle>Molecular pharmacology</jtitle><addtitle>Mol Pharmacol</addtitle><date>1965-07</date><risdate>1965</risdate><volume>1</volume><issue>1</issue><spage>109</spage><epage>112</epage><pages>109-112</pages><issn>0026-895X</issn><eissn>1521-0111</eissn><abstract>A streptomycin dependent mutant was
obtained from wild-type (valine-sensitive)
Escherichia coli K12. This mutant ( E. coli
DK 12) grew exponentially in minimal
medium containing valine (plus dihydrostreptomycin). The inhibition by valine of
the acetohydroxy acid synthetase from
both dependent and parent cells was quantitatively similar. However, the activity of
the acetohydroxy acid synthetase of DK 12
cells was higher than that of the parent
cells. It was concluded that the insensitivity to valine of E. coli DK 12 was the
result of derepression of (acetohydroxy)
acid synthetase in this mutant, the role of
the antibiotic in dependent cells being that
of a "derepressor."</abstract><cop>United States</cop><pub>American Society for Pharmacology and Experimental Therapeutics</pub><pmid>5319648</pmid><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0026-895X |
| ispartof | Molecular pharmacology, 1965-07, Vol.1 (1), p.109-112 |
| issn | 0026-895X 1521-0111 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Culture Media Enzyme Repression Escherichia coli - drug effects Escherichia coli - metabolism In Vitro Techniques Ligases Molecular Biology Mutation Streptomycin - pharmacology Valine - pharmacology |
| title | Insensitivity to Valine of Streptomycin-Dependent Escherichia coli K12 |
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