Determination of ethylenethiourea in urine by liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for monitoring background levels in the general population
This study reports a sensitive analytical method suitable for the quantitative analysis of ethylenethiourea (ETU) in human urine and its application to samples from the general population. Sample preparation involved the use of diatomaceous earth extraction columns to remove matrix interferences. Qu...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-10, Vol.878 (27), p.2563-2566 |
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| container_issue | 27 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Jones, Kate Patel, Katan Cocker, John Bevan, Ruth Levy, Len |
| description | This study reports a sensitive analytical method suitable for the quantitative analysis of ethylenethiourea (ETU) in human urine and its application to samples from the general population. Sample preparation involved the use of diatomaceous earth extraction columns to remove matrix interferences. Quantification was achieved by liquid chromatography–mass spectrometry using positive ion atmospheric pressure chemical ionisation. Within-day and between-day variability of 14% (
n
=
10) and 11% (
n
=
6), respectively, were obtained at 98
nmol/l (10
μg
l
−1). The assay was linear over the investigated range 2.5–245
nmol/l, with a limit of detection of 2.5
nmol/l. The method was applied to monitoring background levels of ETU in urine samples from the general population in the UK. Results obtained from 361 spot samples contained ETU levels ranging from less than the detection limit (54% of samples) to a maximum of 15.8
μmol/mol creatinine (14.3
μg/g creatinine). The 95th percentile was 5.7
μmol/mol creatinine (5.2
μg/g creatinine). |
| doi_str_mv | 10.1016/j.jchromb.2009.10.028 |
| format | Article |
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n
=
10) and 11% (
n
=
6), respectively, were obtained at 98
nmol/l (10
μg
l
−1). The assay was linear over the investigated range 2.5–245
nmol/l, with a limit of detection of 2.5
nmol/l. The method was applied to monitoring background levels of ETU in urine samples from the general population in the UK. Results obtained from 361 spot samples contained ETU levels ranging from less than the detection limit (54% of samples) to a maximum of 15.8
μmol/mol creatinine (14.3
μg/g creatinine). The 95th percentile was 5.7
μmol/mol creatinine (5.2
μg/g creatinine).</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2009.10.028</identifier><identifier>PMID: 19914152</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Atmospheric Pressure ; Biological monitoring ; Chromatography ; Chromatography, High Pressure Liquid - methods ; Creatinine ; Dithiocarbamates ; Ethylenethiourea ; Ethylenethiourea - analysis ; Humans ; Liquid chromatography ; Liquids ; Mass spectrometry ; Mass Spectrometry - methods ; Monitoring ; Pesticide residues ; Quantitative analysis ; Reproducibility of Results ; Spectrometry ; Spectroscopy ; Urine</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-10, Vol.878 (27), p.2563-2566</ispartof><rights>2009</rights><rights>Crown Copyright © 2009. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-fb626a65ee7772176d2ec9d1a3fd9099ce828342d53f7a26d587cb3cf521c58c3</citedby><cites>FETCH-LOGICAL-c477t-fb626a65ee7772176d2ec9d1a3fd9099ce828342d53f7a26d587cb3cf521c58c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2009.10.028$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19914152$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jones, Kate</creatorcontrib><creatorcontrib>Patel, Katan</creatorcontrib><creatorcontrib>Cocker, John</creatorcontrib><creatorcontrib>Bevan, Ruth</creatorcontrib><creatorcontrib>Levy, Len</creatorcontrib><title>Determination of ethylenethiourea in urine by liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for monitoring background levels in the general population</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>This study reports a sensitive analytical method suitable for the quantitative analysis of ethylenethiourea (ETU) in human urine and its application to samples from the general population. Sample preparation involved the use of diatomaceous earth extraction columns to remove matrix interferences. Quantification was achieved by liquid chromatography–mass spectrometry using positive ion atmospheric pressure chemical ionisation. Within-day and between-day variability of 14% (
n
=
10) and 11% (
n
=
6), respectively, were obtained at 98
nmol/l (10
μg
l
−1). The assay was linear over the investigated range 2.5–245
nmol/l, with a limit of detection of 2.5
nmol/l. The method was applied to monitoring background levels of ETU in urine samples from the general population in the UK. Results obtained from 361 spot samples contained ETU levels ranging from less than the detection limit (54% of samples) to a maximum of 15.8
μmol/mol creatinine (14.3
μg/g creatinine). The 95th percentile was 5.7
μmol/mol creatinine (5.2
μg/g creatinine).</description><subject>Atmospheric Pressure</subject><subject>Biological monitoring</subject><subject>Chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Creatinine</subject><subject>Dithiocarbamates</subject><subject>Ethylenethiourea</subject><subject>Ethylenethiourea - analysis</subject><subject>Humans</subject><subject>Liquid chromatography</subject><subject>Liquids</subject><subject>Mass spectrometry</subject><subject>Mass Spectrometry - methods</subject><subject>Monitoring</subject><subject>Pesticide residues</subject><subject>Quantitative analysis</subject><subject>Reproducibility of Results</subject><subject>Spectrometry</subject><subject>Spectroscopy</subject><subject>Urine</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1TAQhSNERUvhEUDescrFP3HsrBAqUJAqdQMSO8uxJze-JHFqO5Wy4x14Hl6mT1LfH6nLrmY0-uac0ZyieEfwhmBSf9xtdqYPfmw3FOMmzzaYyhfFBZGClUzUv1_mngtcYsroefE6xh3GRGDBXhXnpGlIRTi9KP5_gQRhdJNOzk_IdwhSvw4w5eL8EkAjN6EluAlQu6LB3S3OooOzTn4b9NyvD3__6TT6OPcQnEFzgBjzZqZgdEYPKCu7eDDI6KhjRHEGk7IGpLCizgc0ZiT5bLNFrTZ_tsEvk0UD3MMQ9xekHtA2XxWy3OznZTjIvSnOOj1EeHuql8Wvb19_Xn0vb26vf1x9vilNJUQqu7amta45gBCCElFbCqaxRLPONrhpDEgqWUUtZ53QtLZcCtMy03FKDJeGXRYfjrpz8HcLxKRGFw0Mg57AL1HJqqmkaCR7lhSck5oJwTPJj6QJPsYAnZqDG3VYFcFqH7HaqVPEah_xfpwjznvvTw5LO4J92jplmoFPRyC_Du4dBBWNg8mAdSF_XVnvnrF4BIfaw-w</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Jones, Kate</creator><creator>Patel, Katan</creator><creator>Cocker, John</creator><creator>Bevan, Ruth</creator><creator>Levy, Len</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TB</scope><scope>8FD</scope><scope>FR3</scope></search><sort><creationdate>20101001</creationdate><title>Determination of ethylenethiourea in urine by liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for monitoring background levels in the general population</title><author>Jones, Kate ; Patel, Katan ; Cocker, John ; Bevan, Ruth ; Levy, Len</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-fb626a65ee7772176d2ec9d1a3fd9099ce828342d53f7a26d587cb3cf521c58c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Atmospheric Pressure</topic><topic>Biological monitoring</topic><topic>Chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Creatinine</topic><topic>Dithiocarbamates</topic><topic>Ethylenethiourea</topic><topic>Ethylenethiourea - analysis</topic><topic>Humans</topic><topic>Liquid chromatography</topic><topic>Liquids</topic><topic>Mass spectrometry</topic><topic>Mass Spectrometry - methods</topic><topic>Monitoring</topic><topic>Pesticide residues</topic><topic>Quantitative analysis</topic><topic>Reproducibility of Results</topic><topic>Spectrometry</topic><topic>Spectroscopy</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jones, Kate</creatorcontrib><creatorcontrib>Patel, Katan</creatorcontrib><creatorcontrib>Cocker, John</creatorcontrib><creatorcontrib>Bevan, Ruth</creatorcontrib><creatorcontrib>Levy, Len</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jones, Kate</au><au>Patel, Katan</au><au>Cocker, John</au><au>Bevan, Ruth</au><au>Levy, Len</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of ethylenethiourea in urine by liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for monitoring background levels in the general population</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>878</volume><issue>27</issue><spage>2563</spage><epage>2566</epage><pages>2563-2566</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>This study reports a sensitive analytical method suitable for the quantitative analysis of ethylenethiourea (ETU) in human urine and its application to samples from the general population. Sample preparation involved the use of diatomaceous earth extraction columns to remove matrix interferences. Quantification was achieved by liquid chromatography–mass spectrometry using positive ion atmospheric pressure chemical ionisation. Within-day and between-day variability of 14% (
n
=
10) and 11% (
n
=
6), respectively, were obtained at 98
nmol/l (10
μg
l
−1). The assay was linear over the investigated range 2.5–245
nmol/l, with a limit of detection of 2.5
nmol/l. The method was applied to monitoring background levels of ETU in urine samples from the general population in the UK. Results obtained from 361 spot samples contained ETU levels ranging from less than the detection limit (54% of samples) to a maximum of 15.8
μmol/mol creatinine (14.3
μg/g creatinine). The 95th percentile was 5.7
μmol/mol creatinine (5.2
μg/g creatinine).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19914152</pmid><doi>10.1016/j.jchromb.2009.10.028</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 1570-0232 |
| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2010-10, Vol.878 (27), p.2563-2566 |
| issn | 1570-0232 1873-376X |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Atmospheric Pressure Biological monitoring Chromatography Chromatography, High Pressure Liquid - methods Creatinine Dithiocarbamates Ethylenethiourea Ethylenethiourea - analysis Humans Liquid chromatography Liquids Mass spectrometry Mass Spectrometry - methods Monitoring Pesticide residues Quantitative analysis Reproducibility of Results Spectrometry Spectroscopy Urine |
| title | Determination of ethylenethiourea in urine by liquid chromatography–atmospheric pressure chemical ionisation–mass spectrometry for monitoring background levels in the general population |
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