A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells
We have developed a simple multi-layer microfluidic device by integrating a polydimethyl siloxane (PDMS) microfluidic channel and a porous membrane substrate to culture and analyze the renal tubular cells. As a model cell type, primary rat inner medullary collecting duct (IMCD) cells were cultured i...
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| Veröffentlicht in: | Lab on a chip 2010-01, Vol.10 (1), p.36-42 |
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| creator | Jang, Kyung-Jin Suh, Kahp-Yang |
| description | We have developed a simple multi-layer microfluidic device by integrating a polydimethyl siloxane (PDMS) microfluidic channel and a porous membrane substrate to culture and analyze the renal tubular cells. As a model cell type, primary rat inner medullary collecting duct (IMCD) cells were cultured inside the channel. To generate in vivo-like tubular environments for the cells, a fluidic shear stress of 1 dyn/cm(2) was applied for 5 hours, allowing for optimal fluidic conditions for the cultured cells, as verified by enhanced cell polarization, cytoskeletal reorganization, and molecular transport by hormonal stimulations. These results suggest that the microfluidic device presented here is useful for resembling an in vivo renal tubule system and has potential applications in drug screening and advanced tissue engineering. |
| doi_str_mv | 10.1039/b907515a |
| format | Article |
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As a model cell type, primary rat inner medullary collecting duct (IMCD) cells were cultured inside the channel. To generate in vivo-like tubular environments for the cells, a fluidic shear stress of 1 dyn/cm(2) was applied for 5 hours, allowing for optimal fluidic conditions for the cultured cells, as verified by enhanced cell polarization, cytoskeletal reorganization, and molecular transport by hormonal stimulations. 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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cells, Cultured Channels Culture Devices Dimethylpolysiloxanes - chemistry Ducts Equipment Design Fluidics Kidney Tubules, Collecting - cytology Lab-On-A-Chip Devices Male Membranes, Artificial Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Microfluidics Multilayers Porosity Rats Rats, Sprague-Dawley Surgical implants |
| title | A multi-layer microfluidic device for efficient culture and analysis of renal tubular cells |
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