Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125
The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanol...
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| Veröffentlicht in: | Chinese journal of chemical engineering 2010-10, Vol.18 (5), p.870-875 |
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| creator | 李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟 |
| description | The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles. |
| doi_str_mv | 10.1016/S1004-9541(09)60141-5 |
| format | Article |
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The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. 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The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.</description><subject>Candida</subject><subject>Crude oil</subject><subject>Esters</subject><subject>Fatty acids</subject><subject>immobilized lipase</subject><subject>Lipase</subject><subject>methanolysis</subject><subject>Methyl alcohol</subject><subject>optimization</subject><subject>Oryza sativa</subject><subject>Rice bran oil</subject><subject>Solvents</subject><subject>transesterification</subject><subject>假丝酵母</subject><subject>固定化脂肪酶</subject><subject>最佳条件</subject><subject>生物柴油</subject><subject>米糠油</subject><subject>脂肪酸甲酯</subject><subject>酶法合成</subject><issn>1004-9541</issn><issn>2210-321X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqFkUtvFDEQhC0EEkvgJyBZXAiHCe3XzPiEktUGIgVFIiBxszx2O2uYx8b2gia_nkk24gjqQ1--qlZ1EfKawQkDVr-_ZgCy0kqyY9DvamCSVeoJWXHOoBKcfX9KVn-R5-RFzj8AOLSsXZF-M97Ngy3R0et5LFvMMdMp0HNbykxPXfT0M5bt3NNNLpgyDWka6DrtPdIv0SE9S3akV7Gnv2PZ0othmLrYxzv0dG1HH72leXdCta4YVy_Js2D7jK8e9xH5dr75uv5UXV59vFifXlZOAiuVWlJJ67QPHFDpgHVt204LxSUAa7yX2mHgQXd-GVCOOa18KyDUXdPVrTgibw--uzTd7jEXM8TssO_tiNM-m1Zq2UohYCGP_0mypmmYbDWIBVUH1KUp54TB7FIcbJoNA3Pfg3nowdw_2YA2Dz0Yteg-HHS4JP4VMZnsIo4OfUzoivFT_K_Dm8fL22m8uY3jjems-xlij0aohte1rMUf20mZNg</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟</creator><general>Elsevier B.V</general><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>L7M</scope><scope>M7N</scope></search><sort><creationdate>20101001</creationdate><title>Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125</title><author>李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-51014ac9df20e59fe66a8b935240017dd49cef2f9bdbdb05c1c95d830f6b7b683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Candida</topic><topic>Crude oil</topic><topic>Esters</topic><topic>Fatty acids</topic><topic>immobilized lipase</topic><topic>Lipase</topic><topic>methanolysis</topic><topic>Methyl alcohol</topic><topic>optimization</topic><topic>Oryza sativa</topic><topic>Rice bran oil</topic><topic>Solvents</topic><topic>transesterification</topic><topic>假丝酵母</topic><topic>固定化脂肪酶</topic><topic>最佳条件</topic><topic>生物柴油</topic><topic>米糠油</topic><topic>脂肪酸甲酯</topic><topic>酶法合成</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Chinese journal of chemical engineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>李政 邓利 鲁吉珂 郭小雷 杨自信 谭天伟</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125</atitle><jtitle>Chinese journal of chemical engineering</jtitle><addtitle>Chinese Journal of Chemical Engineering</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>18</volume><issue>5</issue><spage>870</spage><epage>875</epage><pages>870-875</pages><issn>1004-9541</issn><eissn>2210-321X</eissn><abstract>The non-edible crude rice bran oil was extracted from white rice bran, and then was catalyzed by immobilized lipase for biodiesel production in this study. The effects of water content, oil/methanol molar ratio, temperature, enzyme amount, solvent,number of methanol added times and two-step methanolysis by using Candida sp. 99-125 as catalyst were investigated. The optimal conditions for processing 1 g rice bran oil were： 0.2 g immobilized lipase, 2 ml n-hexane as solvent, 20% water based on the rice bran oil mass, temperature of 40 °C and two-step addition of methanol. As a result, the fatty acid methyl esters yield was 87.4%. The immobilized lipase was proved to be stable when it was used repeatedly for 7 cycles.</abstract><pub>Elsevier B.V</pub><doi>10.1016/S1004-9541(09)60141-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Candida Crude oil Esters Fatty acids immobilized lipase Lipase methanolysis Methyl alcohol optimization Oryza sativa Rice bran oil Solvents transesterification 假丝酵母 固定化脂肪酶 最佳条件 生物柴油 米糠油 脂肪酸甲酯 酶法合成 |
| title | Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125 |
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