An aspartic acid protease from common bean is expressed ‘on call’ during water stress and early recovery
A cDNA encoding a putative aspartic acid protease precursor (PvAP1) was cloned from the leaves of common bean (Phaseolus vulgaris). Sequence analysis showed that PvAP1 presents all the characteristic features of phytepsins, the typical plant APs. PvAP1 gene expression was tightly regulated by water...
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| Veröffentlicht in: | Journal of plant physiology 2010-12, Vol.167 (18), p.1606-1612 |
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| creator | Contour-Ansel, Dominique Torres-Franklin, Maria Lucia Zuily-Fodil, Yasmine Cruz de Carvalho, Maria Helena |
| description | A cDNA encoding a putative aspartic acid protease precursor (PvAP1) was cloned from the leaves of common bean (Phaseolus vulgaris). Sequence analysis showed that PvAP1 presents all the characteristic features of phytepsins, the typical plant APs. PvAP1 gene expression was tightly regulated by water stress, being significantly up-regulated under mild water stress (Ψw = −1.0 MPa) for the drought-susceptible cultivar (Carioca) and moderate water stress (Ψw = −1.5 MPa) for the more drought-tolerant cultivar (IPA). Protein gel blotting analysis under water stress revealed the presence of two main bands of calculated MW of 46 and 38 kDa, suggesting proteolytic processing of the enzyme precursor form under drought in both cultivars. Taken together, our results suggest that water stress regulates PvAP1 activity both at the transcriptional and post-transcriptional levels, and that the response occurs earlier and is stronger in the drought-susceptible cultivar. |
| doi_str_mv | 10.1016/j.jplph.2010.06.018 |
| format | Article |
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Sequence analysis showed that PvAP1 presents all the characteristic features of phytepsins, the typical plant APs. PvAP1 gene expression was tightly regulated by water stress, being significantly up-regulated under mild water stress (Ψw = −1.0 MPa) for the drought-susceptible cultivar (Carioca) and moderate water stress (Ψw = −1.5 MPa) for the more drought-tolerant cultivar (IPA). Protein gel blotting analysis under water stress revealed the presence of two main bands of calculated MW of 46 and 38 kDa, suggesting proteolytic processing of the enzyme precursor form under drought in both cultivars. Taken together, our results suggest that water stress regulates PvAP1 activity both at the transcriptional and post-transcriptional levels, and that the response occurs earlier and is stronger in the drought-susceptible cultivar.</description><identifier>ISSN: 0176-1617</identifier><identifier>EISSN: 1618-1328</identifier><identifier>DOI: 10.1016/j.jplph.2010.06.018</identifier><identifier>PMID: 20705361</identifier><identifier>CODEN: JPPHEY</identifier><language>eng</language><publisher>Munich: Elsevier GmbH</publisher><subject>Aspartic acid ; Aspartic Acid Proteases - classification ; Aspartic Acid Proteases - genetics ; Aspartic Acid Proteases - metabolism ; Bands ; Beans ; Biological and medical sciences ; complementary DNA ; cultivars ; drought ; Drought tolerance ; Droughts ; Fundamental and applied biological sciences. Psychology ; gels ; Gene expression ; gene expression regulation ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Plant - physiology ; Immunoblotting ; leaves ; Mathematical analysis ; Phaseolus - enzymology ; Phaseolus - metabolism ; Phaseolus vulgaris ; Phylogeny ; Phytepsins ; Plant physiology and development ; Plant Proteins - classification ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Precursors ; Protease ; proteinases ; Proteolysis ; sequence analysis ; Stresses ; transcription (genetics) ; water stress</subject><ispartof>Journal of plant physiology, 2010-12, Vol.167 (18), p.1606-1612</ispartof><rights>2010 Elsevier GmbH</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c468t-19028371c7592fa54990290d56cb6baf35d0271a65d4af72f49ba38bf1a6d38f3</citedby><cites>FETCH-LOGICAL-c468t-19028371c7592fa54990290d56cb6baf35d0271a65d4af72f49ba38bf1a6d38f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jplph.2010.06.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23508044$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20705361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Contour-Ansel, Dominique</creatorcontrib><creatorcontrib>Torres-Franklin, Maria Lucia</creatorcontrib><creatorcontrib>Zuily-Fodil, Yasmine</creatorcontrib><creatorcontrib>Cruz de Carvalho, Maria Helena</creatorcontrib><title>An aspartic acid protease from common bean is expressed ‘on call’ during water stress and early recovery</title><title>Journal of plant physiology</title><addtitle>J Plant Physiol</addtitle><description>A cDNA encoding a putative aspartic acid protease precursor (PvAP1) was cloned from the leaves of common bean (Phaseolus vulgaris). Sequence analysis showed that PvAP1 presents all the characteristic features of phytepsins, the typical plant APs. PvAP1 gene expression was tightly regulated by water stress, being significantly up-regulated under mild water stress (Ψw = −1.0 MPa) for the drought-susceptible cultivar (Carioca) and moderate water stress (Ψw = −1.5 MPa) for the more drought-tolerant cultivar (IPA). Protein gel blotting analysis under water stress revealed the presence of two main bands of calculated MW of 46 and 38 kDa, suggesting proteolytic processing of the enzyme precursor form under drought in both cultivars. Taken together, our results suggest that water stress regulates PvAP1 activity both at the transcriptional and post-transcriptional levels, and that the response occurs earlier and is stronger in the drought-susceptible cultivar.</description><subject>Aspartic acid</subject><subject>Aspartic Acid Proteases - classification</subject><subject>Aspartic Acid Proteases - genetics</subject><subject>Aspartic Acid Proteases - metabolism</subject><subject>Bands</subject><subject>Beans</subject><subject>Biological and medical sciences</subject><subject>complementary DNA</subject><subject>cultivars</subject><subject>drought</subject><subject>Drought tolerance</subject><subject>Droughts</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gels</subject><subject>Gene expression</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Immunoblotting</subject><subject>leaves</subject><subject>Mathematical analysis</subject><subject>Phaseolus - enzymology</subject><subject>Phaseolus - metabolism</subject><subject>Phaseolus vulgaris</subject><subject>Phylogeny</subject><subject>Phytepsins</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - classification</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Precursors</subject><subject>Protease</subject><subject>proteinases</subject><subject>Proteolysis</subject><subject>sequence analysis</subject><subject>Stresses</subject><subject>transcription (genetics)</subject><subject>water stress</subject><issn>0176-1617</issn><issn>1618-1328</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtuFDEQhi0EIkPgBEjgDWLVQ7nd7XYvWEQRLykSC8jaqrbLwaN-YfcEZpdjwPVyEjzMACtgZfnXV2VXfYw9FrAWINSLzXoz9_OndQk5AbUGoe-wlVBCF0KW-i5bgWhUkYPmhD1IaQP5Xmt5n52U0EAtlVix_mzkmGaMS7AcbXB8jtNCmIj7OA3cTsMwjbwjHHlInL7OkVIix29vvuXcYt_f3nznbhvDeMW_4EKRp2XPcBwdJ4z9jkey0zXF3UN2z2Of6NHxPGWXr199PH9bXLx_8-787KKwldJLIVootWyEbeq29FhXbQ5acLWynerQy9pB2QhUtavQN6Wv2g6l7nyOnNRenrLnh755ls9bSosZQrLU9zjStE1GV22loWrK_5KNknlnCnQm5YG0cUopkjdzDAPGnRFg9j7Mxvz0YfY-DCiTfeSqJ8f-224g97vml4AMPDsCmPIyfcTRhvSHkzXkn1aZe3rgPE4Gr2JmLj_klyoAUEJp9VdCgmgrKeWeeHkgKO_-OlA0yQYaLbmQDS3GTeGfw_wAb9W92g</recordid><startdate>20101215</startdate><enddate>20101215</enddate><creator>Contour-Ansel, Dominique</creator><creator>Torres-Franklin, Maria Lucia</creator><creator>Zuily-Fodil, Yasmine</creator><creator>Cruz de Carvalho, Maria Helena</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope></search><sort><creationdate>20101215</creationdate><title>An aspartic acid protease from common bean is expressed ‘on call’ during water stress and early recovery</title><author>Contour-Ansel, Dominique ; Torres-Franklin, Maria Lucia ; Zuily-Fodil, Yasmine ; Cruz de Carvalho, Maria Helena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c468t-19028371c7592fa54990290d56cb6baf35d0271a65d4af72f49ba38bf1a6d38f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aspartic acid</topic><topic>Aspartic Acid Proteases - classification</topic><topic>Aspartic Acid Proteases - genetics</topic><topic>Aspartic Acid Proteases - metabolism</topic><topic>Bands</topic><topic>Beans</topic><topic>Biological and medical sciences</topic><topic>complementary DNA</topic><topic>cultivars</topic><topic>drought</topic><topic>Drought tolerance</topic><topic>Droughts</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gels</topic><topic>Gene expression</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Immunoblotting</topic><topic>leaves</topic><topic>Mathematical analysis</topic><topic>Phaseolus - enzymology</topic><topic>Phaseolus - metabolism</topic><topic>Phaseolus vulgaris</topic><topic>Phylogeny</topic><topic>Phytepsins</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - classification</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Precursors</topic><topic>Protease</topic><topic>proteinases</topic><topic>Proteolysis</topic><topic>sequence analysis</topic><topic>Stresses</topic><topic>transcription (genetics)</topic><topic>water stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Contour-Ansel, Dominique</creatorcontrib><creatorcontrib>Torres-Franklin, Maria Lucia</creatorcontrib><creatorcontrib>Zuily-Fodil, Yasmine</creatorcontrib><creatorcontrib>Cruz de Carvalho, Maria Helena</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of plant physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Contour-Ansel, Dominique</au><au>Torres-Franklin, Maria Lucia</au><au>Zuily-Fodil, Yasmine</au><au>Cruz de Carvalho, Maria Helena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An aspartic acid protease from common bean is expressed ‘on call’ during water stress and early recovery</atitle><jtitle>Journal of plant physiology</jtitle><addtitle>J Plant Physiol</addtitle><date>2010-12-15</date><risdate>2010</risdate><volume>167</volume><issue>18</issue><spage>1606</spage><epage>1612</epage><pages>1606-1612</pages><issn>0176-1617</issn><eissn>1618-1328</eissn><coden>JPPHEY</coden><abstract>A cDNA encoding a putative aspartic acid protease precursor (PvAP1) was cloned from the leaves of common bean (Phaseolus vulgaris). Sequence analysis showed that PvAP1 presents all the characteristic features of phytepsins, the typical plant APs. PvAP1 gene expression was tightly regulated by water stress, being significantly up-regulated under mild water stress (Ψw = −1.0 MPa) for the drought-susceptible cultivar (Carioca) and moderate water stress (Ψw = −1.5 MPa) for the more drought-tolerant cultivar (IPA). Protein gel blotting analysis under water stress revealed the presence of two main bands of calculated MW of 46 and 38 kDa, suggesting proteolytic processing of the enzyme precursor form under drought in both cultivars. Taken together, our results suggest that water stress regulates PvAP1 activity both at the transcriptional and post-transcriptional levels, and that the response occurs earlier and is stronger in the drought-susceptible cultivar.</abstract><cop>Munich</cop><pub>Elsevier GmbH</pub><pmid>20705361</pmid><doi>10.1016/j.jplph.2010.06.018</doi><tpages>7</tpages></addata></record> |
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| subjects | Aspartic acid Aspartic Acid Proteases - classification Aspartic Acid Proteases - genetics Aspartic Acid Proteases - metabolism Bands Beans Biological and medical sciences complementary DNA cultivars drought Drought tolerance Droughts Fundamental and applied biological sciences. Psychology gels Gene expression gene expression regulation Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology Immunoblotting leaves Mathematical analysis Phaseolus - enzymology Phaseolus - metabolism Phaseolus vulgaris Phylogeny Phytepsins Plant physiology and development Plant Proteins - classification Plant Proteins - genetics Plant Proteins - metabolism Precursors Protease proteinases Proteolysis sequence analysis Stresses transcription (genetics) water stress |
| title | An aspartic acid protease from common bean is expressed ‘on call’ during water stress and early recovery |
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