Cell delivery with genipin crosslinked gelatin microspheres in hydrogel/microcarrier composite
Tissue engineering using injectable cellular constructs such as hydrogel is an emerging field in regenerative medicine. Common non-adhesive hydrogels have the advantages of biocompatibility and injectability but modest cell settlement and viability due to its hydrophilic nature which makes it a chal...
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Veröffentlicht in: | Composites science and technology 2010-11, Vol.70 (13), p.1909-1914 |
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creator | Lau, Ting Ting Wang, Chunming Wang, Dong-An |
description | Tissue engineering using injectable cellular constructs such as hydrogel is an emerging field in regenerative medicine. Common non-adhesive hydrogels have the advantages of biocompatibility and injectability but modest cell settlement and viability due to its hydrophilic nature which makes it a challenge for effective and sustainable applications in cell-based therapies. Recently, a hydrogel/microcarrier (GC) model has been developed to provide cells with ‘anchors’ to support cell adhesion within the hydrogel bulk. However, complex fabrications steps and minimal bio-degradability of the microcarrier have limited the spatial growth of the cells. This study aims to overcome challenges of the current GC model through the use of bio-degradable and bio-adhesive gelatin microspheres stabilized with natural crosslinking agent, genipin. This genipin crosslinked microcarrier is capable of maintaining high viability and the stretched morphology of the cells when encapsulated in the hydrogel bulk. This suggests that the microcarrier developed is suitable for GC composite model and has potential applications in biomedical applications. |
doi_str_mv | 10.1016/j.compscitech.2010.05.015 |
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Common non-adhesive hydrogels have the advantages of biocompatibility and injectability but modest cell settlement and viability due to its hydrophilic nature which makes it a challenge for effective and sustainable applications in cell-based therapies. Recently, a hydrogel/microcarrier (GC) model has been developed to provide cells with ‘anchors’ to support cell adhesion within the hydrogel bulk. However, complex fabrications steps and minimal bio-degradability of the microcarrier have limited the spatial growth of the cells. This study aims to overcome challenges of the current GC model through the use of bio-degradable and bio-adhesive gelatin microspheres stabilized with natural crosslinking agent, genipin. This genipin crosslinked microcarrier is capable of maintaining high viability and the stretched morphology of the cells when encapsulated in the hydrogel bulk. This suggests that the microcarrier developed is suitable for GC composite model and has potential applications in biomedical applications.</description><identifier>ISSN: 0266-3538</identifier><identifier>EISSN: 1879-1050</identifier><identifier>DOI: 10.1016/j.compscitech.2010.05.015</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>A. Gelatin microspheres ; A. Genipin ; A. Hydrogel ; Cellular ; Crosslinking ; E. Cell delivery ; Gelatins ; Genipin ; Hydrogels ; Microspheres ; Regenerative ; Viability</subject><ispartof>Composites science and technology, 2010-11, Vol.70 (13), p.1909-1914</ispartof><rights>2010 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-65b02ca0bf5a5e2d70401ecc08808b247f90ff4b95c40c31fbbe63af9aadc4573</citedby><cites>FETCH-LOGICAL-c394t-65b02ca0bf5a5e2d70401ecc08808b247f90ff4b95c40c31fbbe63af9aadc4573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.compscitech.2010.05.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids></links><search><creatorcontrib>Lau, Ting Ting</creatorcontrib><creatorcontrib>Wang, Chunming</creatorcontrib><creatorcontrib>Wang, Dong-An</creatorcontrib><title>Cell delivery with genipin crosslinked gelatin microspheres in hydrogel/microcarrier composite</title><title>Composites science and technology</title><description>Tissue engineering using injectable cellular constructs such as hydrogel is an emerging field in regenerative medicine. Common non-adhesive hydrogels have the advantages of biocompatibility and injectability but modest cell settlement and viability due to its hydrophilic nature which makes it a challenge for effective and sustainable applications in cell-based therapies. Recently, a hydrogel/microcarrier (GC) model has been developed to provide cells with ‘anchors’ to support cell adhesion within the hydrogel bulk. However, complex fabrications steps and minimal bio-degradability of the microcarrier have limited the spatial growth of the cells. This study aims to overcome challenges of the current GC model through the use of bio-degradable and bio-adhesive gelatin microspheres stabilized with natural crosslinking agent, genipin. This genipin crosslinked microcarrier is capable of maintaining high viability and the stretched morphology of the cells when encapsulated in the hydrogel bulk. This suggests that the microcarrier developed is suitable for GC composite model and has potential applications in biomedical applications.</description><subject>A. Gelatin microspheres</subject><subject>A. Genipin</subject><subject>A. Hydrogel</subject><subject>Cellular</subject><subject>Crosslinking</subject><subject>E. Cell delivery</subject><subject>Gelatins</subject><subject>Genipin</subject><subject>Hydrogels</subject><subject>Microspheres</subject><subject>Regenerative</subject><subject>Viability</subject><issn>0266-3538</issn><issn>1879-1050</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><recordid>eNqNUMtOwzAQtBBIlMI_hBOnpOvETuIjqnhJSFzgiuU4a-KSF3Za1L_HaTlw5LTamdnVzBByTSGhQPPVJtFDN3ptJ9RNkkLAgSdA-QlZ0LIQMQUOp2QBaZ7HGc_Kc3Lh_QYACi7SBXlfY9tGNbZ2h24ffdupiT6wt6PtI-0G71vbf2IdsFZNAevsjI4NOvRR2Jt97YZArg6EVs5ZdNHsafDB0yU5M6r1ePU7l-Tt_u51_Rg_vzw8rW-fY50JNsU5ryDVCirDFce0LoABRa2hLKGsUlYYAcawSnDNQGfUVBXmmTJCqVozXmRLcnP8O7rha4t-kp31OkRTPQ5bL0smWAlZyoNSHJWHdA6NHJ3tlNtLCnKuVG7kn0rlXKkELkOl4XZ9vMUQZReCyqDCXmNtHepJ1oP9x5cfS-KI9g</recordid><startdate>20101115</startdate><enddate>20101115</enddate><creator>Lau, Ting Ting</creator><creator>Wang, Chunming</creator><creator>Wang, Dong-An</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20101115</creationdate><title>Cell delivery with genipin crosslinked gelatin microspheres in hydrogel/microcarrier composite</title><author>Lau, Ting Ting ; Wang, Chunming ; Wang, Dong-An</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-65b02ca0bf5a5e2d70401ecc08808b247f90ff4b95c40c31fbbe63af9aadc4573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>A. Gelatin microspheres</topic><topic>A. Genipin</topic><topic>A. Hydrogel</topic><topic>Cellular</topic><topic>Crosslinking</topic><topic>E. Cell delivery</topic><topic>Gelatins</topic><topic>Genipin</topic><topic>Hydrogels</topic><topic>Microspheres</topic><topic>Regenerative</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lau, Ting Ting</creatorcontrib><creatorcontrib>Wang, Chunming</creatorcontrib><creatorcontrib>Wang, Dong-An</creatorcontrib><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Composites science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lau, Ting Ting</au><au>Wang, Chunming</au><au>Wang, Dong-An</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell delivery with genipin crosslinked gelatin microspheres in hydrogel/microcarrier composite</atitle><jtitle>Composites science and technology</jtitle><date>2010-11-15</date><risdate>2010</risdate><volume>70</volume><issue>13</issue><spage>1909</spage><epage>1914</epage><pages>1909-1914</pages><issn>0266-3538</issn><eissn>1879-1050</eissn><abstract>Tissue engineering using injectable cellular constructs such as hydrogel is an emerging field in regenerative medicine. Common non-adhesive hydrogels have the advantages of biocompatibility and injectability but modest cell settlement and viability due to its hydrophilic nature which makes it a challenge for effective and sustainable applications in cell-based therapies. Recently, a hydrogel/microcarrier (GC) model has been developed to provide cells with ‘anchors’ to support cell adhesion within the hydrogel bulk. However, complex fabrications steps and minimal bio-degradability of the microcarrier have limited the spatial growth of the cells. This study aims to overcome challenges of the current GC model through the use of bio-degradable and bio-adhesive gelatin microspheres stabilized with natural crosslinking agent, genipin. This genipin crosslinked microcarrier is capable of maintaining high viability and the stretched morphology of the cells when encapsulated in the hydrogel bulk. This suggests that the microcarrier developed is suitable for GC composite model and has potential applications in biomedical applications.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.compscitech.2010.05.015</doi><tpages>6</tpages></addata></record> |
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subjects | A. Gelatin microspheres A. Genipin A. Hydrogel Cellular Crosslinking E. Cell delivery Gelatins Genipin Hydrogels Microspheres Regenerative Viability |
title | Cell delivery with genipin crosslinked gelatin microspheres in hydrogel/microcarrier composite |
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