Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin
Objective: The aim of this study was to evaluate the effects of intense pulsed light (IPL) and ultraviolet A (UVA) irradiation on the expression of matrix metalloproteases (MMPs), tissue inhibitor of metalloproteinase 1 (TIMP-1), and extracellular matrix (ECM) in human skin, and to confirm the relat...
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| Veröffentlicht in: | Photomedicine and laser surgery 2011-02, Vol.29 (2), p.97-103 |
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| creator | Gu, Weijie Liu, Wei Yang, Xueqin Zhao, Xiaozhong Yuan, Xiaoying Ma, Huimin Tian, Yan Meng, Rusong |
| description | Objective:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) and ultraviolet A (UVA) irradiation on the expression of matrix metalloproteases (MMPs), tissue inhibitor of metalloproteinase 1 (TIMP-1), and extracellular matrix (ECM) in human skin, and to confirm the relative mechanism.
Background Data:
IPL has been widely used to treat photoaged skin but the underlying mechanism remains unknown.
Methods:
Human buttock skin was irradiated by repetitive suberythemal doses of UVA and therapeutic doses of IPL. Skin biopsies were taken and the protein/mRNA expression of MMP-1, -3, -9, and -12 and TIMP-1 was examined by immunohistochemistry, image analysis, and real-time quantitative polymerase chain reaction (qRT-PCR).
Results:
Compared with normal control skin, both IPL and UVA irradiation induced a general elevation of MMP-1, -3, -9, and -12 and TIMP-1 at protein/mRNA levels, with some differences in the MMP expression patterns, e.g., a remarkable increase of MMP-1, -3, and -12 in UVA-exposed skin, while lower MMP-1, -3, and -12 (
p
|
| doi_str_mv | 10.1089/pho.2010.2765 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_849432813</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A249685130</galeid><sourcerecordid>A249685130</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-ed81ee8503de750e28b0fcd27b0fee419c7fbd11fd61fee059be61ae7935ae043</originalsourceid><addsrcrecordid>eNqFkcFrFDEUxoMotlaPXiXgwdOsycxkJ3NcymoLW1qoPYfM5KWNZpI1yZT2v_eNWwVBKDm85OP3Xr7HR8h7zlacyf7z_i6uaoavuluLF-SYC9FVUgj2crm3fSVaLo_Im5y_M1b3Xd-8Jkc1Zyj38pg8bq2FsWQaLT0PBUIGejX7DIbu3O1doToYeuNL0vcueih0Q2OgF1C093GfYgEXdIb8m9s-IDeC97PXiV7oktwDivsEOTtsc4GezZMO9PqHC2_JK6vxo3dP9YTcfNl-Oz2rdpdfz083u2psWVMqMJIDSMEaA51gUMuB2dHUHRYAXGLs7GA4t2bNUWCiH2DNNeCiQgNrmxPy6TAX3f6cIRc1ubyY1AHinJVs-7apJW-Q_Hggb7UH5YKNyzoLrTZ126-l4A1DavUfCo-ByY0xgHWo_9NQHRrGFHNOYNU-uUmnR8WZWjJUmKFaMlRLhsh_eDI8DxOYv_Sf0BBoDsAi6xC8gwFSeWbsL1sPqbA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>849432813</pqid></control><display><type>article</type><title>Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Gu, Weijie ; Liu, Wei ; Yang, Xueqin ; Zhao, Xiaozhong ; Yuan, Xiaoying ; Ma, Huimin ; Tian, Yan ; Meng, Rusong</creator><creatorcontrib>Gu, Weijie ; Liu, Wei ; Yang, Xueqin ; Zhao, Xiaozhong ; Yuan, Xiaoying ; Ma, Huimin ; Tian, Yan ; Meng, Rusong</creatorcontrib><description>Objective:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) and ultraviolet A (UVA) irradiation on the expression of matrix metalloproteases (MMPs), tissue inhibitor of metalloproteinase 1 (TIMP-1), and extracellular matrix (ECM) in human skin, and to confirm the relative mechanism.
Background Data:
IPL has been widely used to treat photoaged skin but the underlying mechanism remains unknown.
Methods:
Human buttock skin was irradiated by repetitive suberythemal doses of UVA and therapeutic doses of IPL. Skin biopsies were taken and the protein/mRNA expression of MMP-1, -3, -9, and -12 and TIMP-1 was examined by immunohistochemistry, image analysis, and real-time quantitative polymerase chain reaction (qRT-PCR).
Results:
Compared with normal control skin, both IPL and UVA irradiation induced a general elevation of MMP-1, -3, -9, and -12 and TIMP-1 at protein/mRNA levels, with some differences in the MMP expression patterns, e.g., a remarkable increase of MMP-1, -3, and -12 in UVA-exposed skin, while lower MMP-1, -3, and -12 (
p
< 0.01,
p
< 0.01,
p
< 0.01) but higher MMP-9 (
p
< 0.05) levels in IPL-irradiated skin. Meanwhile, increased collagen I fibers (
p
< 0.05) were observed in IPL-irradiated skin, whereas fragments of elastic fibers were found in UVA-exposed skin.
Conclusion:
These findings demonstrated that IPL has similar effects on the expression of MMPs/TIMP-1 in human skin to UVA, but the expression pattern differed from that in UVA-exposed skin, which may account for IPL's photorejuvenation effects.</description><identifier>ISSN: 1549-5418</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2010.2765</identifier><identifier>PMID: 21054198</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Collagen ; Collagen - metabolism ; Elastin - metabolism ; Extracellular Matrix - metabolism ; Gene expression ; Genetic aspects ; Health aspects ; Humans ; Image Processing, Computer-Assisted ; Immunohistochemistry ; Matrix Metalloproteinases - metabolism ; Matrix Metalloproteinases - radiation effects ; Optical properties ; Original Research ; Physiological aspects ; Polymerase Chain Reaction - methods ; Skin ; Skin - metabolism ; Skin - radiation effects ; Tissue Inhibitor of Metalloproteinase-1 - metabolism ; Tissue Inhibitor of Metalloproteinase-1 - radiation effects ; Ultraviolet radiation ; Ultraviolet Therapy</subject><ispartof>Photomedicine and laser surgery, 2011-02, Vol.29 (2), p.97-103</ispartof><rights>2011, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2011 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-ed81ee8503de750e28b0fcd27b0fee419c7fbd11fd61fee059be61ae7935ae043</citedby><cites>FETCH-LOGICAL-c403t-ed81ee8503de750e28b0fcd27b0fee419c7fbd11fd61fee059be61ae7935ae043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21054198$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gu, Weijie</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Yang, Xueqin</creatorcontrib><creatorcontrib>Zhao, Xiaozhong</creatorcontrib><creatorcontrib>Yuan, Xiaoying</creatorcontrib><creatorcontrib>Ma, Huimin</creatorcontrib><creatorcontrib>Tian, Yan</creatorcontrib><creatorcontrib>Meng, Rusong</creatorcontrib><title>Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objective:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) and ultraviolet A (UVA) irradiation on the expression of matrix metalloproteases (MMPs), tissue inhibitor of metalloproteinase 1 (TIMP-1), and extracellular matrix (ECM) in human skin, and to confirm the relative mechanism.
Background Data:
IPL has been widely used to treat photoaged skin but the underlying mechanism remains unknown.
Methods:
Human buttock skin was irradiated by repetitive suberythemal doses of UVA and therapeutic doses of IPL. Skin biopsies were taken and the protein/mRNA expression of MMP-1, -3, -9, and -12 and TIMP-1 was examined by immunohistochemistry, image analysis, and real-time quantitative polymerase chain reaction (qRT-PCR).
Results:
Compared with normal control skin, both IPL and UVA irradiation induced a general elevation of MMP-1, -3, -9, and -12 and TIMP-1 at protein/mRNA levels, with some differences in the MMP expression patterns, e.g., a remarkable increase of MMP-1, -3, and -12 in UVA-exposed skin, while lower MMP-1, -3, and -12 (
p
< 0.01,
p
< 0.01,
p
< 0.01) but higher MMP-9 (
p
< 0.05) levels in IPL-irradiated skin. Meanwhile, increased collagen I fibers (
p
< 0.05) were observed in IPL-irradiated skin, whereas fragments of elastic fibers were found in UVA-exposed skin.
Conclusion:
These findings demonstrated that IPL has similar effects on the expression of MMPs/TIMP-1 in human skin to UVA, but the expression pattern differed from that in UVA-exposed skin, which may account for IPL's photorejuvenation effects.</description><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Elastin - metabolism</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gene expression</subject><subject>Genetic aspects</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immunohistochemistry</subject><subject>Matrix Metalloproteinases - metabolism</subject><subject>Matrix Metalloproteinases - radiation effects</subject><subject>Optical properties</subject><subject>Original Research</subject><subject>Physiological aspects</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Skin</subject><subject>Skin - metabolism</subject><subject>Skin - radiation effects</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - metabolism</subject><subject>Tissue Inhibitor of Metalloproteinase-1 - radiation effects</subject><subject>Ultraviolet radiation</subject><subject>Ultraviolet Therapy</subject><issn>1549-5418</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFrFDEUxoMotlaPXiXgwdOsycxkJ3NcymoLW1qoPYfM5KWNZpI1yZT2v_eNWwVBKDm85OP3Xr7HR8h7zlacyf7z_i6uaoavuluLF-SYC9FVUgj2crm3fSVaLo_Im5y_M1b3Xd-8Jkc1Zyj38pg8bq2FsWQaLT0PBUIGejX7DIbu3O1doToYeuNL0vcueih0Q2OgF1C093GfYgEXdIb8m9s-IDeC97PXiV7oktwDivsEOTtsc4GezZMO9PqHC2_JK6vxo3dP9YTcfNl-Oz2rdpdfz083u2psWVMqMJIDSMEaA51gUMuB2dHUHRYAXGLs7GA4t2bNUWCiH2DNNeCiQgNrmxPy6TAX3f6cIRc1ubyY1AHinJVs-7apJW-Q_Hggb7UH5YKNyzoLrTZ126-l4A1DavUfCo-ByY0xgHWo_9NQHRrGFHNOYNU-uUmnR8WZWjJUmKFaMlRLhsh_eDI8DxOYv_Sf0BBoDsAi6xC8gwFSeWbsL1sPqbA</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>Gu, Weijie</creator><creator>Liu, Wei</creator><creator>Yang, Xueqin</creator><creator>Zhao, Xiaozhong</creator><creator>Yuan, Xiaoying</creator><creator>Ma, Huimin</creator><creator>Tian, Yan</creator><creator>Meng, Rusong</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin</title><author>Gu, Weijie ; Liu, Wei ; Yang, Xueqin ; Zhao, Xiaozhong ; Yuan, Xiaoying ; Ma, Huimin ; Tian, Yan ; Meng, Rusong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-ed81ee8503de750e28b0fcd27b0fee419c7fbd11fd61fee059be61ae7935ae043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Elastin - metabolism</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gene expression</topic><topic>Genetic aspects</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immunohistochemistry</topic><topic>Matrix Metalloproteinases - metabolism</topic><topic>Matrix Metalloproteinases - radiation effects</topic><topic>Optical properties</topic><topic>Original Research</topic><topic>Physiological aspects</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Skin</topic><topic>Skin - metabolism</topic><topic>Skin - radiation effects</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - metabolism</topic><topic>Tissue Inhibitor of Metalloproteinase-1 - radiation effects</topic><topic>Ultraviolet radiation</topic><topic>Ultraviolet Therapy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gu, Weijie</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Yang, Xueqin</creatorcontrib><creatorcontrib>Zhao, Xiaozhong</creatorcontrib><creatorcontrib>Yuan, Xiaoying</creatorcontrib><creatorcontrib>Ma, Huimin</creatorcontrib><creatorcontrib>Tian, Yan</creatorcontrib><creatorcontrib>Meng, Rusong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gu, Weijie</au><au>Liu, Wei</au><au>Yang, Xueqin</au><au>Zhao, Xiaozhong</au><au>Yuan, Xiaoying</au><au>Ma, Huimin</au><au>Tian, Yan</au><au>Meng, Rusong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>29</volume><issue>2</issue><spage>97</spage><epage>103</epage><pages>97-103</pages><issn>1549-5418</issn><eissn>1557-8550</eissn><abstract>Objective:
The aim of this study was to evaluate the effects of intense pulsed light (IPL) and ultraviolet A (UVA) irradiation on the expression of matrix metalloproteases (MMPs), tissue inhibitor of metalloproteinase 1 (TIMP-1), and extracellular matrix (ECM) in human skin, and to confirm the relative mechanism.
Background Data:
IPL has been widely used to treat photoaged skin but the underlying mechanism remains unknown.
Methods:
Human buttock skin was irradiated by repetitive suberythemal doses of UVA and therapeutic doses of IPL. Skin biopsies were taken and the protein/mRNA expression of MMP-1, -3, -9, and -12 and TIMP-1 was examined by immunohistochemistry, image analysis, and real-time quantitative polymerase chain reaction (qRT-PCR).
Results:
Compared with normal control skin, both IPL and UVA irradiation induced a general elevation of MMP-1, -3, -9, and -12 and TIMP-1 at protein/mRNA levels, with some differences in the MMP expression patterns, e.g., a remarkable increase of MMP-1, -3, and -12 in UVA-exposed skin, while lower MMP-1, -3, and -12 (
p
< 0.01,
p
< 0.01,
p
< 0.01) but higher MMP-9 (
p
< 0.05) levels in IPL-irradiated skin. Meanwhile, increased collagen I fibers (
p
< 0.05) were observed in IPL-irradiated skin, whereas fragments of elastic fibers were found in UVA-exposed skin.
Conclusion:
These findings demonstrated that IPL has similar effects on the expression of MMPs/TIMP-1 in human skin to UVA, but the expression pattern differed from that in UVA-exposed skin, which may account for IPL's photorejuvenation effects.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>21054198</pmid><doi>10.1089/pho.2010.2765</doi><tpages>7</tpages></addata></record> |
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| ispartof | Photomedicine and laser surgery, 2011-02, Vol.29 (2), p.97-103 |
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| language | eng |
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| subjects | Collagen Collagen - metabolism Elastin - metabolism Extracellular Matrix - metabolism Gene expression Genetic aspects Health aspects Humans Image Processing, Computer-Assisted Immunohistochemistry Matrix Metalloproteinases - metabolism Matrix Metalloproteinases - radiation effects Optical properties Original Research Physiological aspects Polymerase Chain Reaction - methods Skin Skin - metabolism Skin - radiation effects Tissue Inhibitor of Metalloproteinase-1 - metabolism Tissue Inhibitor of Metalloproteinase-1 - radiation effects Ultraviolet radiation Ultraviolet Therapy |
| title | Effects of Intense Pulsed Light and Ultraviolet A on Metalloproteinases and Extracellular Matrix Expression in Human Skin |
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