Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding
The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. These studies reveal that the complex groove binds to DNA sequences biphasi...
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| Veröffentlicht in: | Chemistry : a European journal 2011-02, Vol.17 (7), p.2089-2098 |
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| container_end_page | 2098 |
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| container_issue | 7 |
| container_start_page | 2089 |
| container_title | Chemistry : a European journal |
| container_volume | 17 |
| creator | Ghosh, Amrita Das, Priyadip Gill, Martin R Kar, Prasenjit Walker, Michael G Thomas, Jim A Das, Amitava |
| description | The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. These studies reveal that the complex groove binds to DNA sequences biphasically, and displays binding constants equivalent to those of high-affinity metallointercalators. The complex also displays preferential binding to AT-rich sequences. Changes in the structure of the coordinated catechol ligand and the incorporation of intercalating ancillary ligands into the complex were found to modulate both the optical-binding response and binding parameters of the system, which indicates that the catechol moiety plays a crucial role in the observed enhancement to binding affinities. |
| doi_str_mv | 10.1002/chem.201002149 |
| format | Article |
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Changes in the structure of the coordinated catechol ligand and the incorporation of intercalating ancillary ligands into the complex were found to modulate both the optical-binding response and binding parameters of the system, which indicates that the catechol moiety plays a crucial role in the observed enhancement to binding affinities.</description><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>DNA - chemistry</subject><subject>Intercalating Agents - chemistry</subject><subject>Ligands</subject><subject>Luminescence</subject><subject>Molecular Structure</subject><subject>Organometallic Compounds - chemistry</subject><subject>Photochemistry</subject><subject>Protein Binding</subject><subject>Pyridines - chemistry</subject><subject>Ruthenium - chemistry</subject><issn>1521-3765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtOwzAQRS0kREthyxJ5ByxS_EiceFm1PCpVgBCsI9d2EiMnDrGDyC_w1SSirOZqdObOnQHgAqMlRojcykrXS4ImjWN-BOY4ITiiKUtm4NT7D4QQZ5SegBnBJIkJS-bg56VywQkZzJeGr_31dnsDo9bZoR06owYLpatbq7-1h6ESASrjWysG6PVnrxupR2H1NG3CAEWjYGXKKhJFYZqpszeNMk0Jg4Oqn3zg5mk1OnWuLytYds6Naw_QGTguhPX6_FAX4P3-7m39GO2eH7br1S5qx9QhwnHGZSKF2DOVyjgrGKIk5SxjBKdFGnOcqkQlSGU8U5IjhVWhKJGYY4QJZnQBrv58286NR_iQ18ZLba1otOt9nsU8JpwiOpKXB7Lf11rlbWdq0Q35__voL2W0cc8</recordid><startdate>20110211</startdate><enddate>20110211</enddate><creator>Ghosh, Amrita</creator><creator>Das, Priyadip</creator><creator>Gill, Martin R</creator><creator>Kar, Prasenjit</creator><creator>Walker, Michael G</creator><creator>Thomas, Jim A</creator><creator>Das, Amitava</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20110211</creationdate><title>Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding</title><author>Ghosh, Amrita ; Das, Priyadip ; Gill, Martin R ; Kar, Prasenjit ; Walker, Michael G ; Thomas, Jim A ; Das, Amitava</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p125t-1489c5caab6d7c48f603279686217f74917d5d50d898dc90d1dfd32c191012163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>DNA - chemistry</topic><topic>Intercalating Agents - chemistry</topic><topic>Ligands</topic><topic>Luminescence</topic><topic>Molecular Structure</topic><topic>Organometallic Compounds - chemistry</topic><topic>Photochemistry</topic><topic>Protein Binding</topic><topic>Pyridines - chemistry</topic><topic>Ruthenium - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghosh, Amrita</creatorcontrib><creatorcontrib>Das, Priyadip</creatorcontrib><creatorcontrib>Gill, Martin R</creatorcontrib><creatorcontrib>Kar, Prasenjit</creatorcontrib><creatorcontrib>Walker, Michael G</creatorcontrib><creatorcontrib>Thomas, Jim A</creatorcontrib><creatorcontrib>Das, Amitava</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Chemistry : a European journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghosh, Amrita</au><au>Das, Priyadip</au><au>Gill, Martin R</au><au>Kar, Prasenjit</au><au>Walker, Michael G</au><au>Thomas, Jim A</au><au>Das, Amitava</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding</atitle><jtitle>Chemistry : a European journal</jtitle><addtitle>Chemistry</addtitle><date>2011-02-11</date><risdate>2011</risdate><volume>17</volume><issue>7</issue><spage>2089</spage><epage>2098</epage><pages>2089-2098</pages><eissn>1521-3765</eissn><abstract>The duplex-DNA binding properties of a nonintercalating polypyridyl ruthenium(II) complex that incorporates a linear extended ligand with a catechol moiety has been probed with a variety of photo- and biophysical techniques. 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| identifier | EISSN: 1521-3765 |
| ispartof | Chemistry : a European journal, 2011-02, Vol.17 (7), p.2089-2098 |
| issn | 1521-3765 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Base Sequence Binding Sites DNA - chemistry Intercalating Agents - chemistry Ligands Luminescence Molecular Structure Organometallic Compounds - chemistry Photochemistry Protein Binding Pyridines - chemistry Ruthenium - chemistry |
| title | Photoactive Ru(II) -polypyridyl complexes that display sequence selectivity and high-affinity binding to duplex DNA through groove binding |
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