Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model
Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneratio...
Gespeichert in:
| Veröffentlicht in: | Journal of neurosurgery 2011-02, Vol.114 (2), p.522-528 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 528 |
|---|---|
| container_issue | 2 |
| container_start_page | 522 |
| container_title | Journal of neurosurgery |
| container_volume | 114 |
| creator | EMEL, Erhan SÖNMEZ ERGÜN, Selma KOTAN, Dilcan BASAR GÜRSOY, Esra PARMAN, Yeşim ZENGIN, Asli NURTEN, Asiye |
| description | Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves.
The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.
The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.
This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration. |
| doi_str_mv | 10.3171/2010.9.jns091928 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_849009163</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>849009163</sourcerecordid><originalsourceid>FETCH-LOGICAL-c325t-ee8b64a47014395bce415a58aadd3ee57cc6112bbbde110de1c93cb264f732683</originalsourceid><addsrcrecordid>eNpF0L9OwzAQBnALgWgp7EzIC2JK8Z_EjUdUFahUiQXm6OJcqIuTFNsBdeMleEGehCCKWO67k3664SPknLOp5DN-Ldiw6emmDUxzLfIDMuZayoQpLQ_JmDEhEsnybEROQtgwxlWqxDEZCc6EZjIfk25R12hioF1NbRt6Z9vE2Rekz757j2tag4md__r4XFJoK7p1ENFhTLw1658rNEC7lgZjIVpDW_RvSI3vw3p4t-n9bggK1EOkTVehOyVHNbiAZ_uckKfbxeP8Plk93C3nN6vESJHFBDEvVQrpjPFU6qw0mPIMshygqiRiNjNGcS7KsqyQczYMo6UphUrrmRQqlxNy9ft367vXHkMsGhsMOgctdn0o8lSzoTMlB3mxl33ZYFVsvW3A74q_jgZwuQcQDLjaQ2ts-HcDyTTL5DfY93gb</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>849009163</pqid></control><display><type>article</type><title>Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>EMEL, Erhan ; SÖNMEZ ERGÜN, Selma ; KOTAN, Dilcan ; BASAR GÜRSOY, Esra ; PARMAN, Yeşim ; ZENGIN, Asli ; NURTEN, Asiye</creator><creatorcontrib>EMEL, Erhan ; SÖNMEZ ERGÜN, Selma ; KOTAN, Dilcan ; BASAR GÜRSOY, Esra ; PARMAN, Yeşim ; ZENGIN, Asli ; NURTEN, Asiye</creatorcontrib><description>Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves.
The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.
The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.
This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.</description><identifier>ISSN: 0022-3085</identifier><identifier>EISSN: 1933-0693</identifier><identifier>DOI: 10.3171/2010.9.jns091928</identifier><identifier>PMID: 21029038</identifier><identifier>CODEN: JONSAC</identifier><language>eng</language><publisher>Charlottesville, VA: American Association of Neurological Surgeons</publisher><subject>Animals ; Axons - drug effects ; Axons - pathology ; Biological and medical sciences ; Insulin-Like Growth Factor I - pharmacology ; Insulin-Like Growth Factor I - therapeutic use ; Medical sciences ; Motor Activity - drug effects ; Motor Activity - physiology ; Nerve Crush ; Nerve Regeneration - drug effects ; Nerve Regeneration - physiology ; Neurosurgery ; Platelet-Rich Plasma ; Random Allocation ; Rats ; Rats, Wistar ; Recovery of Function ; Sciatic Nerve - drug effects ; Sciatic Nerve - injuries ; Sciatic Nerve - pathology ; Sciatic Nerve - physiopathology ; Sciatic Neuropathy - pathology ; Sciatic Neuropathy - physiopathology ; Sciatic Neuropathy - therapy ; Statistics, Nonparametric ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Treatment Outcome</subject><ispartof>Journal of neurosurgery, 2011-02, Vol.114 (2), p.522-528</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c325t-ee8b64a47014395bce415a58aadd3ee57cc6112bbbde110de1c93cb264f732683</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23835905$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21029038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EMEL, Erhan</creatorcontrib><creatorcontrib>SÖNMEZ ERGÜN, Selma</creatorcontrib><creatorcontrib>KOTAN, Dilcan</creatorcontrib><creatorcontrib>BASAR GÜRSOY, Esra</creatorcontrib><creatorcontrib>PARMAN, Yeşim</creatorcontrib><creatorcontrib>ZENGIN, Asli</creatorcontrib><creatorcontrib>NURTEN, Asiye</creatorcontrib><title>Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model</title><title>Journal of neurosurgery</title><addtitle>J Neurosurg</addtitle><description>Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves.
The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.
The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.
This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.</description><subject>Animals</subject><subject>Axons - drug effects</subject><subject>Axons - pathology</subject><subject>Biological and medical sciences</subject><subject>Insulin-Like Growth Factor I - pharmacology</subject><subject>Insulin-Like Growth Factor I - therapeutic use</subject><subject>Medical sciences</subject><subject>Motor Activity - drug effects</subject><subject>Motor Activity - physiology</subject><subject>Nerve Crush</subject><subject>Nerve Regeneration - drug effects</subject><subject>Nerve Regeneration - physiology</subject><subject>Neurosurgery</subject><subject>Platelet-Rich Plasma</subject><subject>Random Allocation</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recovery of Function</subject><subject>Sciatic Nerve - drug effects</subject><subject>Sciatic Nerve - injuries</subject><subject>Sciatic Nerve - pathology</subject><subject>Sciatic Nerve - physiopathology</subject><subject>Sciatic Neuropathy - pathology</subject><subject>Sciatic Neuropathy - physiopathology</subject><subject>Sciatic Neuropathy - therapy</subject><subject>Statistics, Nonparametric</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Treatment Outcome</subject><issn>0022-3085</issn><issn>1933-0693</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0L9OwzAQBnALgWgp7EzIC2JK8Z_EjUdUFahUiQXm6OJcqIuTFNsBdeMleEGehCCKWO67k3664SPknLOp5DN-Ldiw6emmDUxzLfIDMuZayoQpLQ_JmDEhEsnybEROQtgwxlWqxDEZCc6EZjIfk25R12hioF1NbRt6Z9vE2Rekz757j2tag4md__r4XFJoK7p1ENFhTLw1658rNEC7lgZjIVpDW_RvSI3vw3p4t-n9bggK1EOkTVehOyVHNbiAZ_uckKfbxeP8Plk93C3nN6vESJHFBDEvVQrpjPFU6qw0mPIMshygqiRiNjNGcS7KsqyQczYMo6UphUrrmRQqlxNy9ft367vXHkMsGhsMOgctdn0o8lSzoTMlB3mxl33ZYFVsvW3A74q_jgZwuQcQDLjaQ2ts-HcDyTTL5DfY93gb</recordid><startdate>20110201</startdate><enddate>20110201</enddate><creator>EMEL, Erhan</creator><creator>SÖNMEZ ERGÜN, Selma</creator><creator>KOTAN, Dilcan</creator><creator>BASAR GÜRSOY, Esra</creator><creator>PARMAN, Yeşim</creator><creator>ZENGIN, Asli</creator><creator>NURTEN, Asiye</creator><general>American Association of Neurological Surgeons</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20110201</creationdate><title>Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model</title><author>EMEL, Erhan ; SÖNMEZ ERGÜN, Selma ; KOTAN, Dilcan ; BASAR GÜRSOY, Esra ; PARMAN, Yeşim ; ZENGIN, Asli ; NURTEN, Asiye</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c325t-ee8b64a47014395bce415a58aadd3ee57cc6112bbbde110de1c93cb264f732683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Axons - drug effects</topic><topic>Axons - pathology</topic><topic>Biological and medical sciences</topic><topic>Insulin-Like Growth Factor I - pharmacology</topic><topic>Insulin-Like Growth Factor I - therapeutic use</topic><topic>Medical sciences</topic><topic>Motor Activity - drug effects</topic><topic>Motor Activity - physiology</topic><topic>Nerve Crush</topic><topic>Nerve Regeneration - drug effects</topic><topic>Nerve Regeneration - physiology</topic><topic>Neurosurgery</topic><topic>Platelet-Rich Plasma</topic><topic>Random Allocation</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recovery of Function</topic><topic>Sciatic Nerve - drug effects</topic><topic>Sciatic Nerve - injuries</topic><topic>Sciatic Nerve - pathology</topic><topic>Sciatic Nerve - physiopathology</topic><topic>Sciatic Neuropathy - pathology</topic><topic>Sciatic Neuropathy - physiopathology</topic><topic>Sciatic Neuropathy - therapy</topic><topic>Statistics, Nonparametric</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EMEL, Erhan</creatorcontrib><creatorcontrib>SÖNMEZ ERGÜN, Selma</creatorcontrib><creatorcontrib>KOTAN, Dilcan</creatorcontrib><creatorcontrib>BASAR GÜRSOY, Esra</creatorcontrib><creatorcontrib>PARMAN, Yeşim</creatorcontrib><creatorcontrib>ZENGIN, Asli</creatorcontrib><creatorcontrib>NURTEN, Asiye</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurosurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EMEL, Erhan</au><au>SÖNMEZ ERGÜN, Selma</au><au>KOTAN, Dilcan</au><au>BASAR GÜRSOY, Esra</au><au>PARMAN, Yeşim</au><au>ZENGIN, Asli</au><au>NURTEN, Asiye</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model</atitle><jtitle>Journal of neurosurgery</jtitle><addtitle>J Neurosurg</addtitle><date>2011-02-01</date><risdate>2011</risdate><volume>114</volume><issue>2</issue><spage>522</spage><epage>528</epage><pages>522-528</pages><issn>0022-3085</issn><eissn>1933-0693</eissn><coden>JONSAC</coden><abstract>Local administration of insulin-like growth factor-I (IGF-I) has been shown to increase the rate of axon regeneration in crush-injured and freeze-injured rat sciatic nerves. Local administration of platelet-rich plasma (PRP) has been also shown to have a measurable effect on facial nerve regeneration after transection in a rat model. The objective of the study was to compare the effects of locally administered IGF-I and PRP on the parameters of the Sciatic Function Index (SFI), sensory function (SF), axon count, and myelin thickness/axon diameter ratio (G-ratio) in a rat model of crush-injured sciatic nerves.
The right sciatic nerve of Wistar albino rats (24 animals) was crushed using a Yasargil-Phynox aneurysm clip for 45 minutes. All animals were randomly divided into 3 groups: Group 1 (control group) was treated with saline, Group 2 was treated with IGF-I, and Group 3 was treated with PRP. Injections were performed using the tissue expander's injection port with a connecting tube directed at the crush-injured site. Functional recovery was assessed with improvement in the SFI. Recovery of sensory function was using the pinch test. Histopathological examination was performed 3 months after the injury.
The SFI showed an improved functional recovery in the IGF-I-treated animals (Group 2) compared with the saline-treated animals (Group 1) 30 days after the injury. In IGF-I-treated rats, sensory function returned to the baseline level significantly faster than in saline-treated and PRP-treated rats as shown in values between SF-2 and SF-7. The G-ratios were found to be significantly higher in both experimental groups than in the control group.
This study suggests that the application of IGF-I to the crush-injured site may expedite the functional recovery of paralyzed muscle by increasing the rate of axon regeneration.</abstract><cop>Charlottesville, VA</cop><pub>American Association of Neurological Surgeons</pub><pmid>21029038</pmid><doi>10.3171/2010.9.jns091928</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3085 |
| ispartof | Journal of neurosurgery, 2011-02, Vol.114 (2), p.522-528 |
| issn | 0022-3085 1933-0693 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_849009163 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Axons - drug effects Axons - pathology Biological and medical sciences Insulin-Like Growth Factor I - pharmacology Insulin-Like Growth Factor I - therapeutic use Medical sciences Motor Activity - drug effects Motor Activity - physiology Nerve Crush Nerve Regeneration - drug effects Nerve Regeneration - physiology Neurosurgery Platelet-Rich Plasma Random Allocation Rats Rats, Wistar Recovery of Function Sciatic Nerve - drug effects Sciatic Nerve - injuries Sciatic Nerve - pathology Sciatic Nerve - physiopathology Sciatic Neuropathy - pathology Sciatic Neuropathy - physiopathology Sciatic Neuropathy - therapy Statistics, Nonparametric Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Treatment Outcome |
| title | Effects of insulin-like growth factor―I and platelet-rich plasma on sciatic nerve crush injury in a rat model |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T07%3A55%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20insulin-like%20growth%20factor%E2%80%95I%20and%20platelet-rich%20plasma%20on%20sciatic%20nerve%20crush%20injury%20in%20a%20rat%20model&rft.jtitle=Journal%20of%20neurosurgery&rft.au=EMEL,%20Erhan&rft.date=2011-02-01&rft.volume=114&rft.issue=2&rft.spage=522&rft.epage=528&rft.pages=522-528&rft.issn=0022-3085&rft.eissn=1933-0693&rft.coden=JONSAC&rft_id=info:doi/10.3171/2010.9.jns091928&rft_dat=%3Cproquest_pubme%3E849009163%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=849009163&rft_id=info:pmid/21029038&rfr_iscdi=true |